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The level of glucose-6-phosphate dehydrogenase activity strongly influences xylose fermentation and inhibitor sensitivity in recombinant Saccharomyces cerevisiae strains.
Yeast. 2003 Nov; 20(15):1263-72.Y

Abstract

Disruption of the ZWF1 gene encoding glucose-6-phosphate dehydrogenase (G6PDH) has been shown to reduce the xylitol yield and the xylose consumption in the xylose-utilizing recombinant Saccharomyces cerevisiae strain TMB3255. In the present investigation we have studied the influence of different production levels of G6PDH on xylose fermentation. We used a synthetic promoter library and the copper-regulated CUP1 promoter to generate G6PDH-activities between 0% and 179% of the wild-type level. G6PDH-activities of 1% and 6% of the wild-type level resulted in 2.8- and 5.1-fold increase in specific xylose consumption, respectively, compared with the ZWF1-disrupted strain. Both strains exhibited decreased xylitol yields (0.13 and 0.19 g/g xylose) and enhanced ethanol yields (0.36 and 0.34 g/g xylose) compared with the control strain TMB3001 (0.29 g xylitol/g xylose, 0.31 g ethanol/g xylose). Cytoplasmic transhydrogenase (TH) from Azotobacter vinelandii has previously been shown to transfer NADPH and NAD(+) into NADP(+) and NADH, and TH-overproduction resulted in lower xylitol yield and enhanced glycerol yield during xylose utilization. Strains with low G6PDH-activity grew slower in a lignocellulose hydrolysate than the strain with wild-type G6PDH-activity, which suggested that the availability of intracellular NADPH correlated with tolerance towards lignocellulose-derived inhibitors. Low G6PDH-activity strains were also more sensitive to H(2)O(2) than the control strain TMB3001.

Authors+Show Affiliations

Jeppsson M
Department of Applied Microbiology, Lund University, PO Box 124, 221 00 Lund, Sweden.
Johansson B
No affiliation info available
Jensen PR
No affiliation info available
Hahn-Hägerdal B
No affiliation info available
Gorwa-Grauslund MF
No affiliation info available

MeSH

AcetatesBase SequenceCarrier ProteinsCelluloseDNA, FungalEthanolFermentationGene Expression Regulation, FungalGlucosephosphate DehydrogenaseGlycerolLigninMetallothioneinMolecular Sequence DataPolymerase Chain ReactionPromoter Regions, GeneticRecombination, GeneticSaccharomyces cerevisiaeTransformation, GeneticXylitolXylose

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14618564

Citation

Jeppsson, Marie, et al. "The Level of Glucose-6-phosphate Dehydrogenase Activity Strongly Influences Xylose Fermentation and Inhibitor Sensitivity in Recombinant Saccharomyces Cerevisiae Strains." Yeast (Chichester, England), vol. 20, no. 15, 2003, pp. 1263-72.
Jeppsson M, Johansson B, Jensen PR, et al. The level of glucose-6-phosphate dehydrogenase activity strongly influences xylose fermentation and inhibitor sensitivity in recombinant Saccharomyces cerevisiae strains. Yeast. 2003;20(15):1263-72.
Jeppsson, M., Johansson, B., Jensen, P. R., Hahn-Hägerdal, B., & Gorwa-Grauslund, M. F. (2003). The level of glucose-6-phosphate dehydrogenase activity strongly influences xylose fermentation and inhibitor sensitivity in recombinant Saccharomyces cerevisiae strains. Yeast (Chichester, England), 20(15), 1263-72.
Jeppsson M, et al. The Level of Glucose-6-phosphate Dehydrogenase Activity Strongly Influences Xylose Fermentation and Inhibitor Sensitivity in Recombinant Saccharomyces Cerevisiae Strains. Yeast. 2003;20(15):1263-72. PubMed PMID: 14618564.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The level of glucose-6-phosphate dehydrogenase activity strongly influences xylose fermentation and inhibitor sensitivity in recombinant Saccharomyces cerevisiae strains. AU - Jeppsson,Marie, AU - Johansson,Björn, AU - Jensen,Peter Ruhdal, AU - Hahn-Hägerdal,Bärbel, AU - Gorwa-Grauslund,Marie F, PY - 2003/11/18/pubmed PY - 2004/2/5/medline PY - 2003/11/18/entrez SP - 1263 EP - 72 JF - Yeast (Chichester, England) JO - Yeast VL - 20 IS - 15 N2 - Disruption of the ZWF1 gene encoding glucose-6-phosphate dehydrogenase (G6PDH) has been shown to reduce the xylitol yield and the xylose consumption in the xylose-utilizing recombinant Saccharomyces cerevisiae strain TMB3255. In the present investigation we have studied the influence of different production levels of G6PDH on xylose fermentation. We used a synthetic promoter library and the copper-regulated CUP1 promoter to generate G6PDH-activities between 0% and 179% of the wild-type level. G6PDH-activities of 1% and 6% of the wild-type level resulted in 2.8- and 5.1-fold increase in specific xylose consumption, respectively, compared with the ZWF1-disrupted strain. Both strains exhibited decreased xylitol yields (0.13 and 0.19 g/g xylose) and enhanced ethanol yields (0.36 and 0.34 g/g xylose) compared with the control strain TMB3001 (0.29 g xylitol/g xylose, 0.31 g ethanol/g xylose). Cytoplasmic transhydrogenase (TH) from Azotobacter vinelandii has previously been shown to transfer NADPH and NAD(+) into NADP(+) and NADH, and TH-overproduction resulted in lower xylitol yield and enhanced glycerol yield during xylose utilization. Strains with low G6PDH-activity grew slower in a lignocellulose hydrolysate than the strain with wild-type G6PDH-activity, which suggested that the availability of intracellular NADPH correlated with tolerance towards lignocellulose-derived inhibitors. Low G6PDH-activity strains were also more sensitive to H(2)O(2) than the control strain TMB3001. SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/14618564/The_level_of_glucose_6_phosphate_dehydrogenase_activity_strongly_influences_xylose_fermentation_and_inhibitor_sensitivity_in_recombinant_Saccharomyces_cerevisiae_strains_ DB - PRIME DP - Unbound Medicine ER -