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[Modulation of matrix metalloproteinase by dexamethasone in airway remodeling in asthmatic rats].
Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Oct; 34(4):680-3.SD

Abstract

OBJECTIVE

To observe the effect of dexamethasone on airway remodeling and to explore the significance of the balance between matrix metalloproteinase and the tissue inhibitor of metalloproteinase.

METHODS

Thirty male Wistar rats were randomly divided into asthmatic group (n = 10), dexamethasone group (n = 10) and control group (n = 10). Lung tissues were sliced and stained with H. E. The parameters such as bronchial basement membrane perimeter(Pbm), total bronchial wall area(WAt), inner wall area(WAi) and smooth muscle area(WAm), which reflect the thickness of airway wall, were measured by image analysis system. The expression levels of MMP-2 and TIMP-1 mRNA in the lungs were assessed by semiquantitative reverse transcription-polymerase chain reaction.

RESULTS

1. WAt/Pbm, WAi/Pbm and WAm/Pbm in asthmatic group (25.3 +/- 2.1 microns 2/micron, 20.4 +/- 2.3 microns 2/micron, 4.2 +/- 2.0 microns 2/micron, respectively) were significantly higher than those in control group (20.8 +/- 1.3 microns 2/micron, 15.3 +/- 2.1 microns 2/micron, 3.1 +/- 1.1 microns 2/micron) (P < 0.01). The differences between those in control group and those in dexamethasone group (21.3 +/- 2.4 microns 2/micron, 14.2 +/- 2.5 microns 2/micron, 3.2 +/- 1.0 microns 2/micron) were not statistically significant (P > 0.05). 2. MMP-2 and TIMP-1mRNA levels in asthmatic group (0.68 +/- 0.14, 0.56 +/- 0.10) and dexamethasone group (0.37 +/- 0.11, 0.31 +/- 0.10) were significantly higher than those in control group (0.14 +/- 0.03, 0.11 +/- 0.05). The differences between asthmatic group and dexamethasone group were also significant (P < 0.01). 3. There was a significant positive correlation between MMP-2 and TIMP-1 mRNA in control group and dexamethasone group (r = 0.67, 0.58, P < 0.05), but not in asthmatic group (r = 0.24, P > 0.05).

CONCLUSION

Dexamethasone could prevent airway remodeling by downregulating the expression of MMP-2 and TIMP-1 and restoring the balance between MMP-2 and TIMP-1.

Authors+Show Affiliations

Department of Geriatrics, West China Hospital, Sichuan University, Chengdu 610041, China.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

14619580

Citation

Yuan, Yiming, et al. "[Modulation of Matrix Metalloproteinase By Dexamethasone in Airway Remodeling in Asthmatic Rats]." Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition, vol. 34, no. 4, 2003, pp. 680-3.
Yuan Y, Wang Z, Dong B. [Modulation of matrix metalloproteinase by dexamethasone in airway remodeling in asthmatic rats]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2003;34(4):680-3.
Yuan, Y., Wang, Z., & Dong, B. (2003). [Modulation of matrix metalloproteinase by dexamethasone in airway remodeling in asthmatic rats]. Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition, 34(4), 680-3.
Yuan Y, Wang Z, Dong B. [Modulation of Matrix Metalloproteinase By Dexamethasone in Airway Remodeling in Asthmatic Rats]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2003;34(4):680-3. PubMed PMID: 14619580.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Modulation of matrix metalloproteinase by dexamethasone in airway remodeling in asthmatic rats]. AU - Yuan,Yiming, AU - Wang,Zengli, AU - Dong,Birong, PY - 2003/11/19/pubmed PY - 2004/8/4/medline PY - 2003/11/19/entrez SP - 680 EP - 3 JF - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition JO - Sichuan Da Xue Xue Bao Yi Xue Ban VL - 34 IS - 4 N2 - OBJECTIVE: To observe the effect of dexamethasone on airway remodeling and to explore the significance of the balance between matrix metalloproteinase and the tissue inhibitor of metalloproteinase. METHODS: Thirty male Wistar rats were randomly divided into asthmatic group (n = 10), dexamethasone group (n = 10) and control group (n = 10). Lung tissues were sliced and stained with H. E. The parameters such as bronchial basement membrane perimeter(Pbm), total bronchial wall area(WAt), inner wall area(WAi) and smooth muscle area(WAm), which reflect the thickness of airway wall, were measured by image analysis system. The expression levels of MMP-2 and TIMP-1 mRNA in the lungs were assessed by semiquantitative reverse transcription-polymerase chain reaction. RESULTS: 1. WAt/Pbm, WAi/Pbm and WAm/Pbm in asthmatic group (25.3 +/- 2.1 microns 2/micron, 20.4 +/- 2.3 microns 2/micron, 4.2 +/- 2.0 microns 2/micron, respectively) were significantly higher than those in control group (20.8 +/- 1.3 microns 2/micron, 15.3 +/- 2.1 microns 2/micron, 3.1 +/- 1.1 microns 2/micron) (P < 0.01). The differences between those in control group and those in dexamethasone group (21.3 +/- 2.4 microns 2/micron, 14.2 +/- 2.5 microns 2/micron, 3.2 +/- 1.0 microns 2/micron) were not statistically significant (P > 0.05). 2. MMP-2 and TIMP-1mRNA levels in asthmatic group (0.68 +/- 0.14, 0.56 +/- 0.10) and dexamethasone group (0.37 +/- 0.11, 0.31 +/- 0.10) were significantly higher than those in control group (0.14 +/- 0.03, 0.11 +/- 0.05). The differences between asthmatic group and dexamethasone group were also significant (P < 0.01). 3. There was a significant positive correlation between MMP-2 and TIMP-1 mRNA in control group and dexamethasone group (r = 0.67, 0.58, P < 0.05), but not in asthmatic group (r = 0.24, P > 0.05). CONCLUSION: Dexamethasone could prevent airway remodeling by downregulating the expression of MMP-2 and TIMP-1 and restoring the balance between MMP-2 and TIMP-1. SN - 1672-173X UR - https://www.unboundmedicine.com/medline/citation/14619580/[Modulation_of_matrix_metalloproteinase_by_dexamethasone_in_airway_remodeling_in_asthmatic_rats]_ L2 - https://medlineplus.gov/asthma.html DB - PRIME DP - Unbound Medicine ER -