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Partially phosphorylated Pho4 activates transcription of a subset of phosphate-responsive genes.
PLoS Biol. 2003 Nov; 1(2):E28.PB

Abstract

A cell's ability to generate different responses to different levels of stimulus is an important component of an adaptive environmental response. Transcriptional responses are frequently controlled by transcription factors regulated by phosphorylation. We demonstrate that differential phosphorylation of the budding yeast transcription factor Pho4 contributes to differential gene expression. When yeast cells are grown in high-phosphate growth medium, Pho4 is phosphorylated on four critical residues by the cyclin-CDK complex Pho80-Pho85 and is inactivated. When yeast cells are starved for phosphate, Pho4 is dephosphorylated and fully active. In intermediate-phosphate conditions, a form of Pho4 preferentially phosphorylated on one of the four sites accumulates and activates transcription of a subset of phosphate-responsive genes. This Pho4 phosphoform binds differentially to phosphate-responsive promoters and helps to trigger differential gene expression. Our results demonstrate that three transcriptional outputs can be generated by a pathway whose regulation is controlled by one kinase, Pho80-Pho85, and one transcription factor, Pho4. Differential phosphorylation of Pho4 by Pho80-Pho85 produces phosphorylated forms of Pho4 that differ in their ability to activate transcription, contributing to multiple outputs.

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Authors+Show Affiliations

Springer M
Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California, San Francisco, USA.
Wykoff DD
No affiliation info available
Miller N
No affiliation info available
O'Shea EK
No affiliation info available

MeSH

Blotting, NorthernBlotting, WesternChromatin ImmunoprecipitationCulture MediaCyclin-Dependent KinasesCyclinsDNA, ComplementaryDNA-Binding ProteinsGene Expression Regulation, FungalGenes, FungalMicroscopy, FluorescenceModels, BiologicalModels, GeneticMolecular Sequence DataOligonucleotide Array Sequence AnalysisPhosphatesPhosphopeptidesPhosphorylationPromoter Regions, GeneticProtein BindingRepressor ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscription FactorsTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

14624238

Citation

Springer, Michael, et al. "Partially Phosphorylated Pho4 Activates Transcription of a Subset of Phosphate-responsive Genes." PLoS Biology, vol. 1, no. 2, 2003, pp. E28.
Springer M, Wykoff DD, Miller N, et al. Partially phosphorylated Pho4 activates transcription of a subset of phosphate-responsive genes. PLoS Biol. 2003;1(2):E28.
Springer, M., Wykoff, D. D., Miller, N., & O'Shea, E. K. (2003). Partially phosphorylated Pho4 activates transcription of a subset of phosphate-responsive genes. PLoS Biology, 1(2), E28.
Springer M, et al. Partially Phosphorylated Pho4 Activates Transcription of a Subset of Phosphate-responsive Genes. PLoS Biol. 2003;1(2):E28. PubMed PMID: 14624238.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Partially phosphorylated Pho4 activates transcription of a subset of phosphate-responsive genes. AU - Springer,Michael, AU - Wykoff,Dennis D, AU - Miller,Nicole, AU - O'Shea,Erin K, Y1 - 2003/11/17/ PY - 2003/05/19/received PY - 2003/08/18/accepted PY - 2003/11/19/pubmed PY - 2006/1/27/medline PY - 2003/11/19/entrez SP - E28 EP - E28 JF - PLoS biology JO - PLoS Biol VL - 1 IS - 2 N2 - A cell's ability to generate different responses to different levels of stimulus is an important component of an adaptive environmental response. Transcriptional responses are frequently controlled by transcription factors regulated by phosphorylation. We demonstrate that differential phosphorylation of the budding yeast transcription factor Pho4 contributes to differential gene expression. When yeast cells are grown in high-phosphate growth medium, Pho4 is phosphorylated on four critical residues by the cyclin-CDK complex Pho80-Pho85 and is inactivated. When yeast cells are starved for phosphate, Pho4 is dephosphorylated and fully active. In intermediate-phosphate conditions, a form of Pho4 preferentially phosphorylated on one of the four sites accumulates and activates transcription of a subset of phosphate-responsive genes. This Pho4 phosphoform binds differentially to phosphate-responsive promoters and helps to trigger differential gene expression. Our results demonstrate that three transcriptional outputs can be generated by a pathway whose regulation is controlled by one kinase, Pho80-Pho85, and one transcription factor, Pho4. Differential phosphorylation of Pho4 by Pho80-Pho85 produces phosphorylated forms of Pho4 that differ in their ability to activate transcription, contributing to multiple outputs. SN - 1545-7885 UR - https://www.unboundmedicine.com/medline/citation/14624238/Partially_phosphorylated_Pho4_activates_transcription_of_a_subset_of_phosphate_responsive_genes_ DB - PRIME DP - Unbound Medicine ER -