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Sperm supply and egg fertilization in the ostrich (Struthio camelus).
Reprod Domest Anim 2003; 38(6):429-35RD

Abstract

We used egg break-out and spermatozoa trapped in the perivitelline layer of eggs to test the hypothesis that sperm supply and egg fertilization rate are high in the ostrich. Egg fertilization status was determined at break-out by the appearance of the germinal disc (GD) and then the perivitelline layer overlying the GD region was collected to count sperm (SpermOPVL) under fluorescence following staining with 4',6'-diamidino-2-phenyindole (DAPI). The study was carried out on commercial ostrich farms over two laying seasons. In the first year, 229 eggs from nine randomly chosen ostrich pens comprising pairs, trios (two females, one male) and larger groups were collected for 1 week of every month of laying. Eggs contained 253 +/- 18 SpermOPVL/mm2 of the GD (mean +/- SEM; range 0-1330). Egg fertilization rate averaged 89.4 +/- 3.4% and varied from 78.6 to 98.2% between pens. Month had no effect on sperm supply or egg fertilization status. Eggs from paired birds (sex ratio 1 : 1) had less sperm in the GD than the eggs from pens with a higher sex ratio. In the second year, 150 eggs from seven pens, each containing only one male and either one, two or three females, were studied for 2 weeks at the beginning (winter), middle (spring) and end (summer) of laying. Eggs contained 364 +/- 45 SpermOPVL/mm2 of the GD (range 0-2880). Season had no effect on sperm supply or egg fertilization. The number of SpermOPVL varied between pens, assumed to be due to variation between individual males. The number of SpermOPVL increased as the sex ratio increased only when very high-ranking males were excluded from the analysis. Egg fertilization rate was 94.4 +/- 3.1% but varied from 64.0 to 100% between pens. Egg fertilization was not affected by season or sex ratio. Low fertilization rates were observed in two pens and appeared related to the lack of synchrony between timing of laying and sperm production in the first, and lack of mating in the second pen. We conclude that ostrich flocks generally have high rates of egg fertilization and any infertility is associated with lack of sperm supply.

Authors+Show Affiliations

School of Animal Biology, Faculty of Natural and Agricultural Sciences The University of Western Australia, Crawley, Australia.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14629663

Citation

Malecki, I A., and G B. Martin. "Sperm Supply and Egg Fertilization in the Ostrich (Struthio Camelus)." Reproduction in Domestic Animals = Zuchthygiene, vol. 38, no. 6, 2003, pp. 429-35.
Malecki IA, Martin GB. Sperm supply and egg fertilization in the ostrich (Struthio camelus). Reprod Domest Anim. 2003;38(6):429-35.
Malecki, I. A., & Martin, G. B. (2003). Sperm supply and egg fertilization in the ostrich (Struthio camelus). Reproduction in Domestic Animals = Zuchthygiene, 38(6), pp. 429-35.
Malecki IA, Martin GB. Sperm Supply and Egg Fertilization in the Ostrich (Struthio Camelus). Reprod Domest Anim. 2003;38(6):429-35. PubMed PMID: 14629663.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sperm supply and egg fertilization in the ostrich (Struthio camelus). AU - Malecki,I A, AU - Martin,G B, PY - 2003/11/25/pubmed PY - 2004/1/30/medline PY - 2003/11/25/entrez SP - 429 EP - 35 JF - Reproduction in domestic animals = Zuchthygiene JO - Reprod. Domest. Anim. VL - 38 IS - 6 N2 - We used egg break-out and spermatozoa trapped in the perivitelline layer of eggs to test the hypothesis that sperm supply and egg fertilization rate are high in the ostrich. Egg fertilization status was determined at break-out by the appearance of the germinal disc (GD) and then the perivitelline layer overlying the GD region was collected to count sperm (SpermOPVL) under fluorescence following staining with 4',6'-diamidino-2-phenyindole (DAPI). The study was carried out on commercial ostrich farms over two laying seasons. In the first year, 229 eggs from nine randomly chosen ostrich pens comprising pairs, trios (two females, one male) and larger groups were collected for 1 week of every month of laying. Eggs contained 253 +/- 18 SpermOPVL/mm2 of the GD (mean +/- SEM; range 0-1330). Egg fertilization rate averaged 89.4 +/- 3.4% and varied from 78.6 to 98.2% between pens. Month had no effect on sperm supply or egg fertilization status. Eggs from paired birds (sex ratio 1 : 1) had less sperm in the GD than the eggs from pens with a higher sex ratio. In the second year, 150 eggs from seven pens, each containing only one male and either one, two or three females, were studied for 2 weeks at the beginning (winter), middle (spring) and end (summer) of laying. Eggs contained 364 +/- 45 SpermOPVL/mm2 of the GD (range 0-2880). Season had no effect on sperm supply or egg fertilization. The number of SpermOPVL varied between pens, assumed to be due to variation between individual males. The number of SpermOPVL increased as the sex ratio increased only when very high-ranking males were excluded from the analysis. Egg fertilization rate was 94.4 +/- 3.1% but varied from 64.0 to 100% between pens. Egg fertilization was not affected by season or sex ratio. Low fertilization rates were observed in two pens and appeared related to the lack of synchrony between timing of laying and sperm production in the first, and lack of mating in the second pen. We conclude that ostrich flocks generally have high rates of egg fertilization and any infertility is associated with lack of sperm supply. SN - 0936-6768 UR - https://www.unboundmedicine.com/medline/citation/14629663/Sperm_supply_and_egg_fertilization_in_the_ostrich__Struthio_camelus__ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0936-6768&date=2003&volume=38&issue=6&spage=429 DB - PRIME DP - Unbound Medicine ER -