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Loss of pre-B and IgM(+) B cells in the bone marrow after exposure to a mixture of herbicides.
J Toxicol Environ Health A. 2003 Dec 26; 66(24):2299-313.JT

Abstract

This study determined alterations to bone marrow B-cell populations after in vivo exposure to a mixture containing the herbicides 3,4-dichloropropionanilide (propanil) and 2,4-dichlorophenoxyacetic acid (2,4-D) and compared them to the effects of exposure to the individual herbicides. Propanil and 2,4-D are postemergent herbicides that are sold commercially as a mixture. The individual herbicides or the mixture containing propanil and 2,4-D were administered intraperitoneally to C57Bl/6 female mice at doses from 50 to 200 mg herbicide/kg body weight. The mixtures were given in a 1:1 ratio. Flow cytometric analysis was performed to quantitate bone marrow B-cell populations at 1, 2, 7, and 14d posttreatment. Mixture treatment decreased pre-B and immunoglobulin (Ig) M(+) B-cell populations at all doses by 2 d postexposure. The cell populations were still decreased at 7d posttreatment. In contrast, exposure to the individual herbicides only caused decreases in the pre-B and IgM(+) B-cell populations 7d after exposure to the high doses. Previous studies have demonstrated that corticosterone levels are increased by exposure to propanil. Therefore, the glucocorticoid hormone, corticosterone, was investigated as a possible mediator of cell loss in the bone marrow. Treatment with the glucocorticoid receptor antagonist, RU 486, however, did not prevent cell loss in the bone marrow of mice exposed to the mixture of propanil and 2,4-D. This study demonstrates that pre-B and IgM(+) B-cell populations are decreased after exposure to propanil, 2,4-D, or the mixture containing propanil and 2,4-D. Exposure to the mixture had greater toxic effects than the individual herbicides on bone marrow pre-B and IgM(+) B-cell populations, emphasizing the need to study mixture interactions.

Authors+Show Affiliations

Department of Microbiology, Immunology and Cell Biology, Robert C. Byrd Health Sciences Center, West Virginia University, Morgantown, WV 26506, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

14630522

Citation

de la Rosa, Patricia, et al. "Loss of pre-B and IgM(+) B Cells in the Bone Marrow After Exposure to a Mixture of Herbicides." Journal of Toxicology and Environmental Health. Part A, vol. 66, no. 24, 2003, pp. 2299-313.
de la Rosa P, Barnett J, Schafer R. Loss of pre-B and IgM(+) B cells in the bone marrow after exposure to a mixture of herbicides. J Toxicol Environ Health Part A. 2003;66(24):2299-313.
de la Rosa, P., Barnett, J., & Schafer, R. (2003). Loss of pre-B and IgM(+) B cells in the bone marrow after exposure to a mixture of herbicides. Journal of Toxicology and Environmental Health. Part A, 66(24), 2299-313.
de la Rosa P, Barnett J, Schafer R. Loss of pre-B and IgM(+) B Cells in the Bone Marrow After Exposure to a Mixture of Herbicides. J Toxicol Environ Health Part A. 2003 Dec 26;66(24):2299-313. PubMed PMID: 14630522.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Loss of pre-B and IgM(+) B cells in the bone marrow after exposure to a mixture of herbicides. AU - de la Rosa,Patricia, AU - Barnett,John, AU - Schafer,Rosana, PY - 2003/11/25/pubmed PY - 2004/1/7/medline PY - 2003/11/25/entrez SP - 2299 EP - 313 JF - Journal of toxicology and environmental health. Part A JO - J. Toxicol. Environ. Health Part A VL - 66 IS - 24 N2 - This study determined alterations to bone marrow B-cell populations after in vivo exposure to a mixture containing the herbicides 3,4-dichloropropionanilide (propanil) and 2,4-dichlorophenoxyacetic acid (2,4-D) and compared them to the effects of exposure to the individual herbicides. Propanil and 2,4-D are postemergent herbicides that are sold commercially as a mixture. The individual herbicides or the mixture containing propanil and 2,4-D were administered intraperitoneally to C57Bl/6 female mice at doses from 50 to 200 mg herbicide/kg body weight. The mixtures were given in a 1:1 ratio. Flow cytometric analysis was performed to quantitate bone marrow B-cell populations at 1, 2, 7, and 14d posttreatment. Mixture treatment decreased pre-B and immunoglobulin (Ig) M(+) B-cell populations at all doses by 2 d postexposure. The cell populations were still decreased at 7d posttreatment. In contrast, exposure to the individual herbicides only caused decreases in the pre-B and IgM(+) B-cell populations 7d after exposure to the high doses. Previous studies have demonstrated that corticosterone levels are increased by exposure to propanil. Therefore, the glucocorticoid hormone, corticosterone, was investigated as a possible mediator of cell loss in the bone marrow. Treatment with the glucocorticoid receptor antagonist, RU 486, however, did not prevent cell loss in the bone marrow of mice exposed to the mixture of propanil and 2,4-D. This study demonstrates that pre-B and IgM(+) B-cell populations are decreased after exposure to propanil, 2,4-D, or the mixture containing propanil and 2,4-D. Exposure to the mixture had greater toxic effects than the individual herbicides on bone marrow pre-B and IgM(+) B-cell populations, emphasizing the need to study mixture interactions. SN - 1528-7394 UR - https://www.unboundmedicine.com/medline/citation/14630522/Loss_of_pre_B_and_IgM_+__B_cells_in_the_bone_marrow_after_exposure_to_a_mixture_of_herbicides_ L2 - http://www.tandfonline.com/doi/full/10.1080/716100638 DB - PRIME DP - Unbound Medicine ER -