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Identification of biochemical markers linked to neonicotinoid cross resistance in Bemisia tabaci (Hemiptera: Aleyrodidae).
Arch Insect Biochem Physiol. 2003 Dec; 54(4):165-76.AI

Abstract

The whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), is a serious pest in many cropping systems world-wide and occurs in different biotypes. The most widespread one is the B-type, whereas the Q-biotype is nowadays still mostly restricted to Southern Spain. Neonicotinoid cross-resistance is known at a high level in Q-types from Spain and individual samples collected in Italy and Germany. Now we detected for the first time high neonicotinoid cross-resistance in a B-type from Israel. Target site resistance to imidacloprid using [(3)H]imidacloprid in nicotinic acetylcholine receptor (nAChR) binding assays could not be detected in any of these highly resistant strains. The impact of metabolizing enzymes such as esterases, glutathione S-transferases, and cytochrome P450-dependent monooxygenases in neonicotinoid resistance was studied biochemically with artificial substrates. Monooxygenase activity was increased 2-3-fold in moderately resistant strains (RF approximately 30) and even 5-6-fold in highly resistant strains (RF approximately 1,000). Only monooxygenase activity correlated with imidacloprid, thiamethoxam and acetamiprid resistance and, therefore, monooxygenases seem to be the only enzyme system responsible for neonicotinoid resistance in B. tabaci Q- and B-types. The oxidative degradation of imidacloprid in resistant Q-type strains could be confirmed by metabolism studies of [(14)C]imidacloprid in vivo. Five-hydroxy-imidacloprid could be detected as the only main metabolite. The insecticidal activity and binding affinity to nAChR of this compound was 10 times lower than imidacloprid itself in B. tabaci.

Authors+Show Affiliations

Rauch N
Bayer CropScience AG, Research, Global Biology Insecticides, Monheim, Germany.
Nauen R
No affiliation info available

MeSH

AnimalsBiological AssayBiomarkersCarbon RadioisotopesCarboxylic Ester HydrolasesCoumarinsCytochrome P-450 Enzyme SystemGlutathione TransferaseHemipteraImidazolesInsecticide ResistanceInsecticidesLethal Dose 50NeonicotinoidsNitro CompoundsOxazinesPlantsPyridinesRadioligand AssayReceptors, NicotinicThiamethoxamThiazolesTritium

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14635178

Citation

Rauch, Natascha, and Ralf Nauen. "Identification of Biochemical Markers Linked to Neonicotinoid Cross Resistance in Bemisia Tabaci (Hemiptera: Aleyrodidae)." Archives of Insect Biochemistry and Physiology, vol. 54, no. 4, 2003, pp. 165-76.
Rauch N, Nauen R. Identification of biochemical markers linked to neonicotinoid cross resistance in Bemisia tabaci (Hemiptera: Aleyrodidae). Arch Insect Biochem Physiol. 2003;54(4):165-76.
Rauch, N., & Nauen, R. (2003). Identification of biochemical markers linked to neonicotinoid cross resistance in Bemisia tabaci (Hemiptera: Aleyrodidae). Archives of Insect Biochemistry and Physiology, 54(4), 165-76.
Rauch N, Nauen R. Identification of Biochemical Markers Linked to Neonicotinoid Cross Resistance in Bemisia Tabaci (Hemiptera: Aleyrodidae). Arch Insect Biochem Physiol. 2003;54(4):165-76. PubMed PMID: 14635178.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of biochemical markers linked to neonicotinoid cross resistance in Bemisia tabaci (Hemiptera: Aleyrodidae). AU - Rauch,Natascha, AU - Nauen,Ralf, PY - 2003/11/25/pubmed PY - 2004/1/15/medline PY - 2003/11/25/entrez SP - 165 EP - 76 JF - Archives of insect biochemistry and physiology JO - Arch Insect Biochem Physiol VL - 54 IS - 4 N2 - The whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), is a serious pest in many cropping systems world-wide and occurs in different biotypes. The most widespread one is the B-type, whereas the Q-biotype is nowadays still mostly restricted to Southern Spain. Neonicotinoid cross-resistance is known at a high level in Q-types from Spain and individual samples collected in Italy and Germany. Now we detected for the first time high neonicotinoid cross-resistance in a B-type from Israel. Target site resistance to imidacloprid using [(3)H]imidacloprid in nicotinic acetylcholine receptor (nAChR) binding assays could not be detected in any of these highly resistant strains. The impact of metabolizing enzymes such as esterases, glutathione S-transferases, and cytochrome P450-dependent monooxygenases in neonicotinoid resistance was studied biochemically with artificial substrates. Monooxygenase activity was increased 2-3-fold in moderately resistant strains (RF approximately 30) and even 5-6-fold in highly resistant strains (RF approximately 1,000). Only monooxygenase activity correlated with imidacloprid, thiamethoxam and acetamiprid resistance and, therefore, monooxygenases seem to be the only enzyme system responsible for neonicotinoid resistance in B. tabaci Q- and B-types. The oxidative degradation of imidacloprid in resistant Q-type strains could be confirmed by metabolism studies of [(14)C]imidacloprid in vivo. Five-hydroxy-imidacloprid could be detected as the only main metabolite. The insecticidal activity and binding affinity to nAChR of this compound was 10 times lower than imidacloprid itself in B. tabaci. SN - 0739-4462 UR - https://www.unboundmedicine.com/medline/citation/14635178/Identification_of_biochemical_markers_linked_to_neonicotinoid_cross_resistance_in_Bemisia_tabaci__Hemiptera:_Aleyrodidae__ DB - PRIME DP - Unbound Medicine ER -