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Charge transport in DNA duplex/quadruplex conjugates.
Biochemistry 2003; 42(48):14159-65B

Abstract

DNA conjugates containing adjacent duplex and guanine quadruplex assemblies have been designed to explore charge transport into quadruplex architectures. The quadruplex assemblies have been characterized structurally using circular dichroism and by assaying for chemical protection. Using an intercalating rhodium photooxidant, noncovalently bound or tethered to the duplex end, oxidizing radicals are found to be trapped in the folded quadruplex. Damage is observed almost exclusively at the external tetrads of the quadruplex. Little damage of the center tetrad is observed, due most likely to lowered efficiency of radical trapping within the quadruplex core. This pattern of damage is distinct from that observed for repetitive G sequences within duplex DNA. The data indicate, furthermore, that in the conjugates examined, the guanine quadruplex provides a more effective trap than a 5'-GG-3' guanine doublet within duplex DNA. Within these assemblies, sufficient base-base overlap must exist at the duplex/quadruplex junction to allow for charge migration. This funneling of damage to the quadruplex, as well as the unique pattern of damage within the quadruplex, requires consideration with respect to the analysis of oxidative DNA damage within the cell.

Authors+Show Affiliations

Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

14640683

Citation

Delaney, Sarah, and Jacqueline K. Barton. "Charge Transport in DNA Duplex/quadruplex Conjugates." Biochemistry, vol. 42, no. 48, 2003, pp. 14159-65.
Delaney S, Barton JK. Charge transport in DNA duplex/quadruplex conjugates. Biochemistry. 2003;42(48):14159-65.
Delaney, S., & Barton, J. K. (2003). Charge transport in DNA duplex/quadruplex conjugates. Biochemistry, 42(48), pp. 14159-65.
Delaney S, Barton JK. Charge Transport in DNA Duplex/quadruplex Conjugates. Biochemistry. 2003 Dec 9;42(48):14159-65. PubMed PMID: 14640683.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Charge transport in DNA duplex/quadruplex conjugates. AU - Delaney,Sarah, AU - Barton,Jacqueline K, PY - 2003/12/4/pubmed PY - 2004/3/18/medline PY - 2003/12/4/entrez SP - 14159 EP - 65 JF - Biochemistry JO - Biochemistry VL - 42 IS - 48 N2 - DNA conjugates containing adjacent duplex and guanine quadruplex assemblies have been designed to explore charge transport into quadruplex architectures. The quadruplex assemblies have been characterized structurally using circular dichroism and by assaying for chemical protection. Using an intercalating rhodium photooxidant, noncovalently bound or tethered to the duplex end, oxidizing radicals are found to be trapped in the folded quadruplex. Damage is observed almost exclusively at the external tetrads of the quadruplex. Little damage of the center tetrad is observed, due most likely to lowered efficiency of radical trapping within the quadruplex core. This pattern of damage is distinct from that observed for repetitive G sequences within duplex DNA. The data indicate, furthermore, that in the conjugates examined, the guanine quadruplex provides a more effective trap than a 5'-GG-3' guanine doublet within duplex DNA. Within these assemblies, sufficient base-base overlap must exist at the duplex/quadruplex junction to allow for charge migration. This funneling of damage to the quadruplex, as well as the unique pattern of damage within the quadruplex, requires consideration with respect to the analysis of oxidative DNA damage within the cell. SN - 0006-2960 UR - https://www.unboundmedicine.com/medline/citation/14640683/Charge_transport_in_DNA_duplex/quadruplex_conjugates_ L2 - https://dx.doi.org/10.1021/bi0351965 DB - PRIME DP - Unbound Medicine ER -