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Monolithic silica-based capillary reversed-phase liquid chromatography/electrospray mass spectrometry for plant metabolomics.
Anal Chem. 2003 Dec 01; 75(23):6737-40.AC

Abstract

Application of C18 monolithic silica capillary columns in HPLC coupled to ion trap mass spectrometry detection was studied for probing the metabolome of the model plant Arabidopsis thaliana. It could be shown that the use of a long capillary column is an easy and effective approach to reduce ionization suppression by enhanced chromatographic resolution. Several hundred peaks could be detected using a 90-cm capillary column for LC separation and a noise reduction and automatic peak alignment software, which outperformed manual inspection or commercially available mass spectral deconvolution software.

Authors+Show Affiliations

Tolstikov VV
Department Lothar Willmitzer, Max Planck Institute of Molecular Plant Physiology, 14424 Potsdam, Germany.
Lommen A
No affiliation info available
Nakanishi K
No affiliation info available
Tanaka N
No affiliation info available
Fiehn O
No affiliation info available

MeSH

ArabidopsisChromatography, High Pressure LiquidElectrophoresis, CapillaryPlant ExtractsSilicon DioxideSoftwareSpectrometry, Mass, Electrospray IonizationSpectrum Analysis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14640754

Citation

Tolstikov, Vladimir V., et al. "Monolithic Silica-based Capillary Reversed-phase Liquid Chromatography/electrospray Mass Spectrometry for Plant Metabolomics." Analytical Chemistry, vol. 75, no. 23, 2003, pp. 6737-40.
Tolstikov VV, Lommen A, Nakanishi K, et al. Monolithic silica-based capillary reversed-phase liquid chromatography/electrospray mass spectrometry for plant metabolomics. Anal Chem. 2003;75(23):6737-40.
Tolstikov, V. V., Lommen, A., Nakanishi, K., Tanaka, N., & Fiehn, O. (2003). Monolithic silica-based capillary reversed-phase liquid chromatography/electrospray mass spectrometry for plant metabolomics. Analytical Chemistry, 75(23), 6737-40.
Tolstikov VV, et al. Monolithic Silica-based Capillary Reversed-phase Liquid Chromatography/electrospray Mass Spectrometry for Plant Metabolomics. Anal Chem. 2003 Dec 1;75(23):6737-40. PubMed PMID: 14640754.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Monolithic silica-based capillary reversed-phase liquid chromatography/electrospray mass spectrometry for plant metabolomics. AU - Tolstikov,Vladimir V, AU - Lommen,Arjen, AU - Nakanishi,Kazuki, AU - Tanaka,Nobuo, AU - Fiehn,Oliver, PY - 2003/12/3/pubmed PY - 2004/10/20/medline PY - 2003/12/3/entrez SP - 6737 EP - 40 JF - Analytical chemistry JO - Anal Chem VL - 75 IS - 23 N2 - Application of C18 monolithic silica capillary columns in HPLC coupled to ion trap mass spectrometry detection was studied for probing the metabolome of the model plant Arabidopsis thaliana. It could be shown that the use of a long capillary column is an easy and effective approach to reduce ionization suppression by enhanced chromatographic resolution. Several hundred peaks could be detected using a 90-cm capillary column for LC separation and a noise reduction and automatic peak alignment software, which outperformed manual inspection or commercially available mass spectral deconvolution software. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/14640754/Monolithic_silica_based_capillary_reversed_phase_liquid_chromatography/electrospray_mass_spectrometry_for_plant_metabolomics_ DB - PRIME DP - Unbound Medicine ER -
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