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Aging increases neuronal nitric oxide release and superoxide anion generation in mesenteric arteries from spontaneously hypertensive rats.
J Vasc Res. 2003 Nov-Dec; 40(6):509-19.JV

Abstract

We hypothesized that neuronal NO release as well as its bioavailability and vasomotor response could be affected when aging and hypertension are present simultaneously. The neuronal nitric oxide (NO) release, its metabolism and vasomotor response induced by electrical field stimulation was analyzed in isolated segments of endothelium-denuded mesenteric arteries from young and old spontaneously hypertensive rats (SHR). The nitric oxide synthase (NOS) inhibitor N(G)-nitro-arginine-methyl ester (L-NAME) and NOS inhibitor 7-nitroindazole both strengthened electrical field stimulation-elicited contractions more in arteries from young than aged SHR rats. Superoxide dismutase (SOD) potentiated the L-NAME effect in segments from old rats, whereas catalase decreased the contractions induced by electrical field stimulation and noradrenaline but did not modify the L-NAME effect. In noradrenaline-precontracted segments, sodium nitroprusside induced a similar relaxation in arteries from both experimental groups. This relaxation was increased by SOD in old SHR. 8Br cGMP induced greater relaxation in segments from old than from young SHR. Electrical field stimulation induced a tritium release in arteries preincubated with [(3)H]-noradrenaline, that was similar in both young and old SHR rats. Electrical field stimulation induced NO(2)(-) formation, which was greater in segments from old than young SHR rats. Basal cGMP levels and those stimulated with sodium nitroprusside were similar in segments from both groups. Superoxide anion production was greater from old than young SHR rats. Peroxynitrite production induced by electrical field stimulation was only detected in segments from old SHR. The results obtained in mesenteric arteries from old SHR showed increased neuronal NO release and superoxide anion production with respect to those observed in arteries from young SHR rats. This induced decreased NO bioavailability through peroxynitrite formation. The final effect is to decrease the involvement of neuronal NO in electrical field stimulation-induced vasomotor response in arteries from old SHR rats.

Authors+Show Affiliations

Departamentos de Fisiología, Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14646371

Citation

Ferrer, Mercedes, et al. "Aging Increases Neuronal Nitric Oxide Release and Superoxide Anion Generation in Mesenteric Arteries From Spontaneously Hypertensive Rats." Journal of Vascular Research, vol. 40, no. 6, 2003, pp. 509-19.
Ferrer M, Sánchez M, Minoves N, et al. Aging increases neuronal nitric oxide release and superoxide anion generation in mesenteric arteries from spontaneously hypertensive rats. J Vasc Res. 2003;40(6):509-19.
Ferrer, M., Sánchez, M., Minoves, N., Salaices, M., & Balfagón, G. (2003). Aging increases neuronal nitric oxide release and superoxide anion generation in mesenteric arteries from spontaneously hypertensive rats. Journal of Vascular Research, 40(6), 509-19.
Ferrer M, et al. Aging Increases Neuronal Nitric Oxide Release and Superoxide Anion Generation in Mesenteric Arteries From Spontaneously Hypertensive Rats. J Vasc Res. 2003 Nov-Dec;40(6):509-19. PubMed PMID: 14646371.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aging increases neuronal nitric oxide release and superoxide anion generation in mesenteric arteries from spontaneously hypertensive rats. AU - Ferrer,Mercedes, AU - Sánchez,Margarita, AU - Minoves,Nuria, AU - Salaices,Mercedes, AU - Balfagón,Gloria, Y1 - 2003/11/27/ PY - 2002/11/15/received PY - 2003/09/04/accepted PY - 2003/12/3/pubmed PY - 2004/2/27/medline PY - 2003/12/3/entrez SP - 509 EP - 19 JF - Journal of vascular research JO - J Vasc Res VL - 40 IS - 6 N2 - We hypothesized that neuronal NO release as well as its bioavailability and vasomotor response could be affected when aging and hypertension are present simultaneously. The neuronal nitric oxide (NO) release, its metabolism and vasomotor response induced by electrical field stimulation was analyzed in isolated segments of endothelium-denuded mesenteric arteries from young and old spontaneously hypertensive rats (SHR). The nitric oxide synthase (NOS) inhibitor N(G)-nitro-arginine-methyl ester (L-NAME) and NOS inhibitor 7-nitroindazole both strengthened electrical field stimulation-elicited contractions more in arteries from young than aged SHR rats. Superoxide dismutase (SOD) potentiated the L-NAME effect in segments from old rats, whereas catalase decreased the contractions induced by electrical field stimulation and noradrenaline but did not modify the L-NAME effect. In noradrenaline-precontracted segments, sodium nitroprusside induced a similar relaxation in arteries from both experimental groups. This relaxation was increased by SOD in old SHR. 8Br cGMP induced greater relaxation in segments from old than from young SHR. Electrical field stimulation induced a tritium release in arteries preincubated with [(3)H]-noradrenaline, that was similar in both young and old SHR rats. Electrical field stimulation induced NO(2)(-) formation, which was greater in segments from old than young SHR rats. Basal cGMP levels and those stimulated with sodium nitroprusside were similar in segments from both groups. Superoxide anion production was greater from old than young SHR rats. Peroxynitrite production induced by electrical field stimulation was only detected in segments from old SHR. The results obtained in mesenteric arteries from old SHR showed increased neuronal NO release and superoxide anion production with respect to those observed in arteries from young SHR rats. This induced decreased NO bioavailability through peroxynitrite formation. The final effect is to decrease the involvement of neuronal NO in electrical field stimulation-induced vasomotor response in arteries from old SHR rats. SN - 1018-1172 UR - https://www.unboundmedicine.com/medline/citation/14646371/Aging_increases_neuronal_nitric_oxide_release_and_superoxide_anion_generation_in_mesenteric_arteries_from_spontaneously_hypertensive_rats_ L2 - https://www.karger.com?DOI=10.1159/000075183 DB - PRIME DP - Unbound Medicine ER -