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[Effect of Citrus aurantium extract on L-type calcium currents in ventricular myocytes of single guinea pigs].
Hunan Yi Ke Da Xue Xue Bao. 2003 Aug; 28(4):353-6.HY

Abstract

OBJECTIVE

To determine the effect of citrus aurantium extract (CAE) on L-type calcium currents (ICa-L) in ventricular myocytes of guinea pigs.

METHODS

Single myocytes were dissociated by enzymatic dissociation method. The whole-cell patch-clamp recording technique was used to record the change of calcium current after the administration of CAE.

RESULTS

1. CAE 4 x 10(-3), 2 x 10(-2), 4 x 10(-2), and 1 x 10(-1) g/ml increased the ICa-L by 15.89%, 29.01%, 52.03% and 59.76% (P < 0.05), respectively. 2. CAE 2 x 10(-1) and 4 x 10(-1) g/ml decreased the ICa-L by 16.61% and 30.87% (P < 0.05), respectively. CAE changed the ICa-L significantly, but did not change the shape of I-V curves.

CONCLUSIONS

CAE 4 x 10(-3)-1 x 10(-1) g/ml can increase the L-type calcium current in guinea pig ventricular myocytes significantly in a concentration-dependent manner, and promote the opening of calcium channel. CAE 2 x 10(-1)-4 x 10(-1) g/ml may inhibit the L-type calcium channels of ventricular myocytes, and depress the opening of calcium channel.

Authors+Show Affiliations

Fang F
Laboratory of Molecular Pharmacology, Xiangya School of Medicine, Central South University, Changsha, 410078, China.
Dong M
No affiliation info available
Zhu H
No affiliation info available

MeSH

AnimalsCalcium Channels, L-TypeCitrusDrugs, Chinese HerbalGuinea PigsMyocytes, CardiacPatch-Clamp Techniques

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

14653115

Citation

Fang, Fang, et al. "[Effect of Citrus Aurantium Extract On L-type Calcium Currents in Ventricular Myocytes of Single Guinea Pigs]." Hunan Yi Ke Da Xue Xue Bao = Hunan Yike Daxue Xuebao = Bulletin of Hunan Medical University, vol. 28, no. 4, 2003, pp. 353-6.
Fang F, Dong M, Zhu H. [Effect of Citrus aurantium extract on L-type calcium currents in ventricular myocytes of single guinea pigs]. Hunan Yi Ke Da Xue Xue Bao. 2003;28(4):353-6.
Fang, F., Dong, M., & Zhu, H. (2003). [Effect of Citrus aurantium extract on L-type calcium currents in ventricular myocytes of single guinea pigs]. Hunan Yi Ke Da Xue Xue Bao = Hunan Yike Daxue Xuebao = Bulletin of Hunan Medical University, 28(4), 353-6.
Fang F, Dong M, Zhu H. [Effect of Citrus Aurantium Extract On L-type Calcium Currents in Ventricular Myocytes of Single Guinea Pigs]. Hunan Yi Ke Da Xue Xue Bao. 2003;28(4):353-6. PubMed PMID: 14653115.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of Citrus aurantium extract on L-type calcium currents in ventricular myocytes of single guinea pigs]. AU - Fang,Fang, AU - Dong,Min, AU - Zhu,Hong, PY - 2003/12/5/pubmed PY - 2004/4/29/medline PY - 2003/12/5/entrez SP - 353 EP - 6 JF - Hunan yi ke da xue xue bao = Hunan yike daxue xuebao = Bulletin of Hunan Medical University JO - Hunan Yi Ke Da Xue Xue Bao VL - 28 IS - 4 N2 - OBJECTIVE: To determine the effect of citrus aurantium extract (CAE) on L-type calcium currents (ICa-L) in ventricular myocytes of guinea pigs. METHODS: Single myocytes were dissociated by enzymatic dissociation method. The whole-cell patch-clamp recording technique was used to record the change of calcium current after the administration of CAE. RESULTS: 1. CAE 4 x 10(-3), 2 x 10(-2), 4 x 10(-2), and 1 x 10(-1) g/ml increased the ICa-L by 15.89%, 29.01%, 52.03% and 59.76% (P < 0.05), respectively. 2. CAE 2 x 10(-1) and 4 x 10(-1) g/ml decreased the ICa-L by 16.61% and 30.87% (P < 0.05), respectively. CAE changed the ICa-L significantly, but did not change the shape of I-V curves. CONCLUSIONS: CAE 4 x 10(-3)-1 x 10(-1) g/ml can increase the L-type calcium current in guinea pig ventricular myocytes significantly in a concentration-dependent manner, and promote the opening of calcium channel. CAE 2 x 10(-1)-4 x 10(-1) g/ml may inhibit the L-type calcium channels of ventricular myocytes, and depress the opening of calcium channel. SN - 1000-5625 UR - https://www.unboundmedicine.com/medline/citation/14653115/[Effect_of_Citrus_aurantium_extract_on_L_type_calcium_currents_in_ventricular_myocytes_of_single_guinea_pigs]_ DB - PRIME DP - Unbound Medicine ER -