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Down-regulation of survivin expression reversed multidrug resistance in adriamycin-resistant HL-60/ADR cell line.
Acta Pharmacol Sin. 2003 Dec; 24(12):1235-40.AP

Abstract

AIM

To investigate the ability of an antisense RNA eukaryotic expression plasmid pcDNA3.1/survivin in down-regulating the expression level of survivin mRNA and survivin protein and reversed multidrug resistance (MDR) in adriamycin-resistant HL-60/ADR cell line.

METHODS

The expression of survivin mRNA was measured by RT-PCR and the expression of survivin protein was measured by Western blot. Caspase-3 activity was determined by Phar Mingen colorimetric assay kit. Apoptosis was assessed by flow cytometry. The chemosensitivity of HL-60/ADR cells to adriamycin (ADR) was measured by MTT assay.

RESULTS

pcDNA3.1/survivin down-regulated the expression level of survivin mRNA and survivin protein obviously, and induced apoptosis of HL-60/ADR cells in a time-dependent manner during 12-48 h. After transient transfection with pcDNA3.1/survivin for 48 h, survivin mRNA decreased by 67 %, survivin protein decreased by 57 %, caspase-3 activity increased 4.37 times, and the apoptosis rate increased by 4.41 % compared with control. Compared with ADR alone, pcDNA3.1/survivin significantly reversed MDR in HL-60/ADR cells, the chemosensitivity of HL-60/ADR cells to ADR was increased to 5.36 folds.

CONCLUSION

pcDNA3.1/survivin down-regulated the expression level of survivin mRNA and survivin protein obviously, the threshold of apoptosis was decreased and MDR was reversed.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14653950

Citation

Wang, Lei, et al. "Down-regulation of Survivin Expression Reversed Multidrug Resistance in Adriamycin-resistant HL-60/ADR Cell Line." Acta Pharmacologica Sinica, vol. 24, no. 12, 2003, pp. 1235-40.
Wang L, Zhang GM, Feng ZH. Down-regulation of survivin expression reversed multidrug resistance in adriamycin-resistant HL-60/ADR cell line. Acta Pharmacol Sin. 2003;24(12):1235-40.
Wang, L., Zhang, G. M., & Feng, Z. H. (2003). Down-regulation of survivin expression reversed multidrug resistance in adriamycin-resistant HL-60/ADR cell line. Acta Pharmacologica Sinica, 24(12), 1235-40.
Wang L, Zhang GM, Feng ZH. Down-regulation of Survivin Expression Reversed Multidrug Resistance in Adriamycin-resistant HL-60/ADR Cell Line. Acta Pharmacol Sin. 2003;24(12):1235-40. PubMed PMID: 14653950.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Down-regulation of survivin expression reversed multidrug resistance in adriamycin-resistant HL-60/ADR cell line. AU - Wang,Lei, AU - Zhang,Gui-Mei, AU - Feng,Zuo-Hua, PY - 2003/12/5/pubmed PY - 2004/8/7/medline PY - 2003/12/5/entrez SP - 1235 EP - 40 JF - Acta pharmacologica Sinica JO - Acta Pharmacol Sin VL - 24 IS - 12 N2 - AIM: To investigate the ability of an antisense RNA eukaryotic expression plasmid pcDNA3.1/survivin in down-regulating the expression level of survivin mRNA and survivin protein and reversed multidrug resistance (MDR) in adriamycin-resistant HL-60/ADR cell line. METHODS: The expression of survivin mRNA was measured by RT-PCR and the expression of survivin protein was measured by Western blot. Caspase-3 activity was determined by Phar Mingen colorimetric assay kit. Apoptosis was assessed by flow cytometry. The chemosensitivity of HL-60/ADR cells to adriamycin (ADR) was measured by MTT assay. RESULTS: pcDNA3.1/survivin down-regulated the expression level of survivin mRNA and survivin protein obviously, and induced apoptosis of HL-60/ADR cells in a time-dependent manner during 12-48 h. After transient transfection with pcDNA3.1/survivin for 48 h, survivin mRNA decreased by 67 %, survivin protein decreased by 57 %, caspase-3 activity increased 4.37 times, and the apoptosis rate increased by 4.41 % compared with control. Compared with ADR alone, pcDNA3.1/survivin significantly reversed MDR in HL-60/ADR cells, the chemosensitivity of HL-60/ADR cells to ADR was increased to 5.36 folds. CONCLUSION: pcDNA3.1/survivin down-regulated the expression level of survivin mRNA and survivin protein obviously, the threshold of apoptosis was decreased and MDR was reversed. SN - 1671-4083 UR - https://www.unboundmedicine.com/medline/citation/14653950/Down_regulation_of_survivin_expression_reversed_multidrug_resistance_in_adriamycin_resistant_HL_60/ADR_cell_line_ L2 - http://www.chinaphar.com/1671-4083/24/1235.pdf DB - PRIME DP - Unbound Medicine ER -