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Production of multiple cytokines and induction of cachexia in athymic nude mice by a new anaplastic thyroid carcinoma cell line.
J Endocrinol 2003; 179(3):387-94JE

Abstract

An anaplastic thyroid cancer cell line, Thena, was recently established in our laboratory following radical thyroidectomy of a patient with anaplastic thyroid cancer. Microscopically, Thena cells were spindle-shaped or small round cells. Thena cells were reactive with cytokeratin AE1/AE3 antibodies, epithelial membrane antigen, interleukin (IL)-6, epithelial growth factor receptor, transforming growth factor (TGF)-alpha, vascular endothelial growth factor, and vimentin. Thena cells secreted high levels of IL-6, leukemia inhibitor factor (LIF), tumor necrosis factor (TNF)-alpha, and TGF-beta1 in the culture supernatants, as determined by enzyme-linked immunosorbent assay. When subcutaneously injected with Thena cells, athymic nude mice developed tumor masses in the skin within 2 weeks. Furthermore, Thena cells induced cachexia in these tumor-bearing mice. High levels of human IL-6, LIF and TGF-beta1 were detected in the mouse sera. To our knowledge, the Thena cell line is the first thyroid cancer cell line reported to induce cachexia in nude mice. This cachectic animal model is worthy of further study to explore the treatment of thyroid cancer-induced cachexia.

Authors+Show Affiliations

Department of Hematology/Oncology, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan. chwang@adm.cgmh.org.twNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14656208

Citation

Chang, J W-C, et al. "Production of Multiple Cytokines and Induction of Cachexia in Athymic Nude Mice By a New Anaplastic Thyroid Carcinoma Cell Line." The Journal of Endocrinology, vol. 179, no. 3, 2003, pp. 387-94.
Chang JW, Yeh KY, Shen YC, et al. Production of multiple cytokines and induction of cachexia in athymic nude mice by a new anaplastic thyroid carcinoma cell line. J Endocrinol. 2003;179(3):387-94.
Chang, J. W., Yeh, K. Y., Shen, Y. C., Hsieh, J. J., Chuang, C. K., Liao, S. K., ... Wang, C. H. (2003). Production of multiple cytokines and induction of cachexia in athymic nude mice by a new anaplastic thyroid carcinoma cell line. The Journal of Endocrinology, 179(3), pp. 387-94.
Chang JW, et al. Production of Multiple Cytokines and Induction of Cachexia in Athymic Nude Mice By a New Anaplastic Thyroid Carcinoma Cell Line. J Endocrinol. 2003;179(3):387-94. PubMed PMID: 14656208.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Production of multiple cytokines and induction of cachexia in athymic nude mice by a new anaplastic thyroid carcinoma cell line. AU - Chang,J W-C, AU - Yeh,K-Y, AU - Shen,Y-C, AU - Hsieh,J-J, AU - Chuang,C-K, AU - Liao,S-K, AU - Tsai,L-H, AU - Wang,C-H, PY - 2003/12/6/pubmed PY - 2004/2/26/medline PY - 2003/12/6/entrez SP - 387 EP - 94 JF - The Journal of endocrinology JO - J. Endocrinol. VL - 179 IS - 3 N2 - An anaplastic thyroid cancer cell line, Thena, was recently established in our laboratory following radical thyroidectomy of a patient with anaplastic thyroid cancer. Microscopically, Thena cells were spindle-shaped or small round cells. Thena cells were reactive with cytokeratin AE1/AE3 antibodies, epithelial membrane antigen, interleukin (IL)-6, epithelial growth factor receptor, transforming growth factor (TGF)-alpha, vascular endothelial growth factor, and vimentin. Thena cells secreted high levels of IL-6, leukemia inhibitor factor (LIF), tumor necrosis factor (TNF)-alpha, and TGF-beta1 in the culture supernatants, as determined by enzyme-linked immunosorbent assay. When subcutaneously injected with Thena cells, athymic nude mice developed tumor masses in the skin within 2 weeks. Furthermore, Thena cells induced cachexia in these tumor-bearing mice. High levels of human IL-6, LIF and TGF-beta1 were detected in the mouse sera. To our knowledge, the Thena cell line is the first thyroid cancer cell line reported to induce cachexia in nude mice. This cachectic animal model is worthy of further study to explore the treatment of thyroid cancer-induced cachexia. SN - 0022-0795 UR - https://www.unboundmedicine.com/medline/citation/14656208/Production_of_multiple_cytokines_and_induction_of_cachexia_in_athymic_nude_mice_by_a_new_anaplastic_thyroid_carcinoma_cell_line_ L2 - https://medlineplus.gov/thyroidcancer.html DB - PRIME DP - Unbound Medicine ER -