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Functional complementation of E. coli secD and secG mutants by Helicobacter pylori homologues.
FEMS Microbiol Lett 2003; 229(1):57-63FM

Abstract

The Sec machinery is one mechanism used by bacteria to translocate proteins across their cytoplasmic membrane. Most of the Sec components have been identified within the important gastric pathogen, Helicobacter pylori, however their functionality has not yet been demonstrated. Here we report the existence of putative homologues to the Sec components yajC (HP1450) and yidC (HP1551), and demonstrate the ability of the H. pylori secD (HP1550) and secG (HP1255) homologues to facilitate inner membrane translocation of the maltose-binding protein MalE, by complementation of the respective secretion-deficient Escherichia coli mutants, thus providing evidence of their functionality.

Authors+Show Affiliations

Institute of Infection, Immunity, and Inflammation, University of Nottingham, Queen's Medical School, C-floor West block, Nottingham NG7 2UH, UK.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14659543

Citation

Fitchen, Nicola, et al. "Functional Complementation of E. Coli secD and secG Mutants By Helicobacter Pylori Homologues." FEMS Microbiology Letters, vol. 229, no. 1, 2003, pp. 57-63.
Fitchen N, Williams P, Hardie KR. Functional complementation of E. coli secD and secG mutants by Helicobacter pylori homologues. FEMS Microbiol Lett. 2003;229(1):57-63.
Fitchen, N., Williams, P., & Hardie, K. R. (2003). Functional complementation of E. coli secD and secG mutants by Helicobacter pylori homologues. FEMS Microbiology Letters, 229(1), pp. 57-63.
Fitchen N, Williams P, Hardie KR. Functional Complementation of E. Coli secD and secG Mutants By Helicobacter Pylori Homologues. FEMS Microbiol Lett. 2003 Dec 5;229(1):57-63. PubMed PMID: 14659543.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional complementation of E. coli secD and secG mutants by Helicobacter pylori homologues. AU - Fitchen,Nicola, AU - Williams,Paul, AU - Hardie,Kim R, PY - 2003/12/9/pubmed PY - 2004/2/24/medline PY - 2003/12/9/entrez SP - 57 EP - 63 JF - FEMS microbiology letters JO - FEMS Microbiol. Lett. VL - 229 IS - 1 N2 - The Sec machinery is one mechanism used by bacteria to translocate proteins across their cytoplasmic membrane. Most of the Sec components have been identified within the important gastric pathogen, Helicobacter pylori, however their functionality has not yet been demonstrated. Here we report the existence of putative homologues to the Sec components yajC (HP1450) and yidC (HP1551), and demonstrate the ability of the H. pylori secD (HP1550) and secG (HP1255) homologues to facilitate inner membrane translocation of the maltose-binding protein MalE, by complementation of the respective secretion-deficient Escherichia coli mutants, thus providing evidence of their functionality. SN - 0378-1097 UR - https://www.unboundmedicine.com/medline/citation/14659543/Functional_complementation_of_E__coli_secD_and_secG_mutants_by_Helicobacter_pylori_homologues_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1016/S0378-1097(03)00786-9 DB - PRIME DP - Unbound Medicine ER -