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Basic and neutral amino acid transport in Aspergillus nidulans.
J Gen Microbiol. 1976 Jan; 92(1):89-96.JG

Abstract

Arginine and methionine transport by Aspergillus nidulans mycelium was investigated. A single uptake system is responsible for the transport of arginine, lysine and ornithine. Transport is energy-dependent and specific for these basic amino acids. The Km value for arginine is 1 X 10(-5) M, and Vmax is 2-8 nmol/mg dry wt/min; Km for lysine is 8 X 10(-6) M; Kt for lysine as inhibitor of arginine uptake is 12 muM, and Ki for ornithine is mM. On minimal medium, methionine is transported with a Km of 0-I mM and Vmax about I nmol/mg dry wt/min; transport is inhibited by azide. Neutral amnio acids such as serine, phenylalanine and leucine are probably transported by the same system, as indicated by their inhibition of methionine uptake and the existence of a mutant specifically impaired in their transport. The recessive mutant nap3, unable to transport neutral amino acids, was isolated as resistant to selenomethionine and p-fluorophenylanine. This mutant has unchanged transport of methionine by general and specific sulphur-regulated permeases.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

1466

Citation

Piotrowska, M, et al. "Basic and Neutral Amino Acid Transport in Aspergillus Nidulans." Journal of General Microbiology, vol. 92, no. 1, 1976, pp. 89-96.
Piotrowska M, Stepień PP, Bartnik E, et al. Basic and neutral amino acid transport in Aspergillus nidulans. J Gen Microbiol. 1976;92(1):89-96.
Piotrowska, M., Stepień, P. P., Bartnik, E., & Zakrzewska, E. (1976). Basic and neutral amino acid transport in Aspergillus nidulans. Journal of General Microbiology, 92(1), 89-96.
Piotrowska M, et al. Basic and Neutral Amino Acid Transport in Aspergillus Nidulans. J Gen Microbiol. 1976;92(1):89-96. PubMed PMID: 1466.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Basic and neutral amino acid transport in Aspergillus nidulans. AU - Piotrowska,M, AU - Stepień,P P, AU - Bartnik,E, AU - Zakrzewska,E, PY - 1976/1/1/pubmed PY - 1976/1/1/medline PY - 1976/1/1/entrez SP - 89 EP - 96 JF - Journal of general microbiology JO - J Gen Microbiol VL - 92 IS - 1 N2 - Arginine and methionine transport by Aspergillus nidulans mycelium was investigated. A single uptake system is responsible for the transport of arginine, lysine and ornithine. Transport is energy-dependent and specific for these basic amino acids. The Km value for arginine is 1 X 10(-5) M, and Vmax is 2-8 nmol/mg dry wt/min; Km for lysine is 8 X 10(-6) M; Kt for lysine as inhibitor of arginine uptake is 12 muM, and Ki for ornithine is mM. On minimal medium, methionine is transported with a Km of 0-I mM and Vmax about I nmol/mg dry wt/min; transport is inhibited by azide. Neutral amnio acids such as serine, phenylalanine and leucine are probably transported by the same system, as indicated by their inhibition of methionine uptake and the existence of a mutant specifically impaired in their transport. The recessive mutant nap3, unable to transport neutral amino acids, was isolated as resistant to selenomethionine and p-fluorophenylanine. This mutant has unchanged transport of methionine by general and specific sulphur-regulated permeases. SN - 0022-1287 UR - https://www.unboundmedicine.com/medline/citation/1466/Basic_and_neutral_amino_acid_transport_in_Aspergillus_nidulans_ DB - PRIME DP - Unbound Medicine ER -