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Analysis of ochratoxin a in coffee by solid-phase cleanup and narrow-bore liquid chromatography-fluorescence detector-mass spectrometry.
J Agric Food Chem. 2003 Dec 17; 51(26):7564-7.JA

Abstract

A method for the analysis of ochratoxin A (OTA) in green and roasted coffee has been developed. OTA was extracted from coffee with 1% NaHCO(3), and the extract was filtered and purified by solid-phase cleanup using a polymeric column that exhibits reversed-phase and anion-exchange functionalities. OTA was analyzed on a narrow-bore reversed-phase C(18) HPLC column with acetonitrile/water (0.1% formic acid) (40:60) as mobile phase and quantified with a fluorescence detector. The presence of OTA in coffee was confirmed by single-quadruple mass spectrometry using an electrospray ionization source. The method has been validated, obtaining a recovery of 82.5% and a detection limit of 0.1 ng/g. It has been applied to 20 coffee samples from various countries and different manufacturers with no detection of OTA.

Authors+Show Affiliations

Ventura M
Analytical Chemistry Department, Institut Químic de Sarrià, Ramon Llull University, Via Augusta 390, 08017 Barcelona, Spain. mvenben@iqs.es
Vallejos C
No affiliation info available
Anaya IA
No affiliation info available
Broto-Puig F
No affiliation info available
Agut M
No affiliation info available
Comellas L
No affiliation info available

MeSH

Chromatography, High Pressure LiquidCoffeaCoffeeHot TemperatureMycotoxinsOchratoxinsPlant ExtractsQuality ControlSeedsSpectrometry, Mass, Electrospray Ionization

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14664508

Citation

Ventura, Meritxell, et al. "Analysis of Ochratoxin a in Coffee By Solid-phase Cleanup and Narrow-bore Liquid Chromatography-fluorescence Detector-mass Spectrometry." Journal of Agricultural and Food Chemistry, vol. 51, no. 26, 2003, pp. 7564-7.
Ventura M, Vallejos C, Anaya IA, et al. Analysis of ochratoxin a in coffee by solid-phase cleanup and narrow-bore liquid chromatography-fluorescence detector-mass spectrometry. J Agric Food Chem. 2003;51(26):7564-7.
Ventura, M., Vallejos, C., Anaya, I. A., Broto-Puig, F., Agut, M., & Comellas, L. (2003). Analysis of ochratoxin a in coffee by solid-phase cleanup and narrow-bore liquid chromatography-fluorescence detector-mass spectrometry. Journal of Agricultural and Food Chemistry, 51(26), 7564-7.
Ventura M, et al. Analysis of Ochratoxin a in Coffee By Solid-phase Cleanup and Narrow-bore Liquid Chromatography-fluorescence Detector-mass Spectrometry. J Agric Food Chem. 2003 Dec 17;51(26):7564-7. PubMed PMID: 14664508.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of ochratoxin a in coffee by solid-phase cleanup and narrow-bore liquid chromatography-fluorescence detector-mass spectrometry. AU - Ventura,Meritxell, AU - Vallejos,Carlos, AU - Anaya,Iván Atilio, AU - Broto-Puig,Francesc, AU - Agut,Montserrat, AU - Comellas,Lluís, PY - 2003/12/11/pubmed PY - 2004/1/22/medline PY - 2003/12/11/entrez SP - 7564 EP - 7 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 51 IS - 26 N2 - A method for the analysis of ochratoxin A (OTA) in green and roasted coffee has been developed. OTA was extracted from coffee with 1% NaHCO(3), and the extract was filtered and purified by solid-phase cleanup using a polymeric column that exhibits reversed-phase and anion-exchange functionalities. OTA was analyzed on a narrow-bore reversed-phase C(18) HPLC column with acetonitrile/water (0.1% formic acid) (40:60) as mobile phase and quantified with a fluorescence detector. The presence of OTA in coffee was confirmed by single-quadruple mass spectrometry using an electrospray ionization source. The method has been validated, obtaining a recovery of 82.5% and a detection limit of 0.1 ng/g. It has been applied to 20 coffee samples from various countries and different manufacturers with no detection of OTA. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/14664508/Analysis_of_ochratoxin_a_in_coffee_by_solid_phase_cleanup_and_narrow_bore_liquid_chromatography_fluorescence_detector_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -