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Cytochrome P450 aromatase in the testis of immature and mature pigs.
Reprod Biol. 2001 Jul; 1(1):51-9.RB

Abstract

Aromatization of androgens into estrogens is performed by a microsomal enzyme, the cytochrome P450 aromatase. A direct approach for identifying the cellular source of aromatase is the use of immunohistochemistry with a specific antibody that recognizes aromatase. The pig presents some unusual features with regard to the synthesis of testosterone and estrogens in the male gonads. In testes from prepubertal males, testosterone level measured radioimmunologically, was lower than in testes from adult pig, while estrogen secretion was relatively high and comparable to that of mature porcine gonads. Immunolocalization of aromatase in testes from both immature and mature pigs was confined to the Leydig cell cytoplasm. The intensity of immunohistochemical staining indicated the presence of unsynchronous Leydig cell population. Other somatic cells and germ cells were negative for aromatase. In control tissue sections, incubated in the absence of the primary antibody or in the presence of normal rabbit serum, no positive staining was observed. Western blot analysis revealed one major band of aromatase about 50-52 kDa in testes from both immature and mature pigs.

Authors+Show Affiliations

Laboratory of Animal Endocrinology and Tissue Culture, Department of Animal Physiology, Institute of Zoology, Jagiellonian University, ul. Ingardena 6, 30-060 Kraków, Poland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14666175

Citation

Fraczek, B, et al. "Cytochrome P450 Aromatase in the Testis of Immature and Mature Pigs." Reproductive Biology, vol. 1, no. 1, 2001, pp. 51-9.
Fraczek B, Kotula-Balak M, Wojtusiak A, et al. Cytochrome P450 aromatase in the testis of immature and mature pigs. Reprod Biol. 2001;1(1):51-9.
Fraczek, B., Kotula-Balak, M., Wojtusiak, A., Pierściński, A., & Bilińska, B. (2001). Cytochrome P450 aromatase in the testis of immature and mature pigs. Reproductive Biology, 1(1), 51-9.
Fraczek B, et al. Cytochrome P450 Aromatase in the Testis of Immature and Mature Pigs. Reprod Biol. 2001;1(1):51-9. PubMed PMID: 14666175.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytochrome P450 aromatase in the testis of immature and mature pigs. AU - Fraczek,B, AU - Kotula-Balak,M, AU - Wojtusiak,A, AU - Pierściński,A, AU - Bilińska,B, PY - 2003/12/11/pubmed PY - 2004/1/17/medline PY - 2003/12/11/entrez SP - 51 EP - 9 JF - Reproductive biology JO - Reprod Biol VL - 1 IS - 1 N2 - Aromatization of androgens into estrogens is performed by a microsomal enzyme, the cytochrome P450 aromatase. A direct approach for identifying the cellular source of aromatase is the use of immunohistochemistry with a specific antibody that recognizes aromatase. The pig presents some unusual features with regard to the synthesis of testosterone and estrogens in the male gonads. In testes from prepubertal males, testosterone level measured radioimmunologically, was lower than in testes from adult pig, while estrogen secretion was relatively high and comparable to that of mature porcine gonads. Immunolocalization of aromatase in testes from both immature and mature pigs was confined to the Leydig cell cytoplasm. The intensity of immunohistochemical staining indicated the presence of unsynchronous Leydig cell population. Other somatic cells and germ cells were negative for aromatase. In control tissue sections, incubated in the absence of the primary antibody or in the presence of normal rabbit serum, no positive staining was observed. Western blot analysis revealed one major band of aromatase about 50-52 kDa in testes from both immature and mature pigs. SN - 1642-431X UR - https://www.unboundmedicine.com/medline/citation/14666175/Cytochrome_P450_aromatase_in_the_testis_of_immature_and_mature_pigs_ DB - PRIME DP - Unbound Medicine ER -