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Generation of a germ cell nuclear factor conditional allele in mice.
Genesis. 2003 Dec; 37(4):172-9.G

Abstract

Two recombination steps in embryonic stem (ES) cells were adopted to generate a floxed Germ Cell Nuclear Factor (GCNF) allele. First, a targeting vector containing a loxP site upstream of exon 4, encoding the DNA binding domain (DBD), and a floxed NeoTK double selection cassette downstream of exon 4 was integrated into the GCNF locus by homologous recombination. Second, a Cre-expressing vector was transiently introduced to remove the floxed NeoTK cassette via site-specific recombination. Heterogeneous ES cell populations were found in a single colony after Cre transfection and were separated using an ES cell re-pick step. Floxed GCNF mice were generated and had normal GCNF expression in the adult gonads. Using the Msx2Cre transgenic mice, the floxed GCNF can be completely deleted in the female germline. Taken together, the floxed GCNF mice were successfully generated and female germline deletion of the floxed GCNF allele was achieved using Msx2Cre mice.

Authors+Show Affiliations

Department of Molecular and Cellular Biology, Baylor College of Medicine, BCMM-M733, Houston, Texas 77030, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

14666510

Citation

Lan, Zi-Jian, et al. "Generation of a Germ Cell Nuclear Factor Conditional Allele in Mice." Genesis (New York, N.Y. : 2000), vol. 37, no. 4, 2003, pp. 172-9.
Lan ZJ, Xu X, Cooney AJ. Generation of a germ cell nuclear factor conditional allele in mice. Genesis. 2003;37(4):172-9.
Lan, Z. J., Xu, X., & Cooney, A. J. (2003). Generation of a germ cell nuclear factor conditional allele in mice. Genesis (New York, N.Y. : 2000), 37(4), 172-9.
Lan ZJ, Xu X, Cooney AJ. Generation of a Germ Cell Nuclear Factor Conditional Allele in Mice. Genesis. 2003;37(4):172-9. PubMed PMID: 14666510.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Generation of a germ cell nuclear factor conditional allele in mice. AU - Lan,Zi-Jian, AU - Xu,Xueping, AU - Cooney,Austin J, PY - 2003/12/11/pubmed PY - 2004/8/26/medline PY - 2003/12/11/entrez SP - 172 EP - 9 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 37 IS - 4 N2 - Two recombination steps in embryonic stem (ES) cells were adopted to generate a floxed Germ Cell Nuclear Factor (GCNF) allele. First, a targeting vector containing a loxP site upstream of exon 4, encoding the DNA binding domain (DBD), and a floxed NeoTK double selection cassette downstream of exon 4 was integrated into the GCNF locus by homologous recombination. Second, a Cre-expressing vector was transiently introduced to remove the floxed NeoTK cassette via site-specific recombination. Heterogeneous ES cell populations were found in a single colony after Cre transfection and were separated using an ES cell re-pick step. Floxed GCNF mice were generated and had normal GCNF expression in the adult gonads. Using the Msx2Cre transgenic mice, the floxed GCNF can be completely deleted in the female germline. Taken together, the floxed GCNF mice were successfully generated and female germline deletion of the floxed GCNF allele was achieved using Msx2Cre mice. SN - 1526-954X UR - https://www.unboundmedicine.com/medline/citation/14666510/Generation_of_a_germ_cell_nuclear_factor_conditional_allele_in_mice_ L2 - https://doi.org/10.1002/gene.10239 DB - PRIME DP - Unbound Medicine ER -