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Determination of gabapentin in human plasma by capillary electrophoresis with laser-induced fluorescence detection and acetonitrile stacking technique.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jan 25; 799(2):265-70.JC

Abstract

A sensitive analytical method for gabapentin [1-(aminomethyl) cyclohexaneacetic acid] (GBP) in human plasma based on capillary electrophoretic separation and laser-induced fluorescence (LIF) detection has been developed. 6-Carboxyfluorescein succinimidyl ester (CFSE) was used for precolumn derivatization of the non-fluorescent drug in plasma. Optimal separation and detection were obtained with an electrophoretic buffer of 50mM sodium borate (pH 9.5) and an air-cooled argon-ion laser (excitation at 488 nm, emission at 520 nm). A calibration curve ranging from 0.3 to 150 microM was shown to be linear. The concentration limit of detection (LOD) in plasma was 60 nM. We also demonstrate how the detection limit can be enhanced by using acetonitrile stacking technique. With stacking, the limit of detection for gabapentin in plasma was 4.8 nM. A calibration curve ranging from 0.03 to 15 microM was shown to be linear. Both the within-day and day-to-day reproducibility and accuracy were </=10.8 and 6.0%, respectively.

Authors+Show Affiliations

Department of Applied Chemistry, Chaoyang University of Technology, Wufeng,Taichung County 413, Taiwan. ychang@mail.cyut.edu.twNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14670745

Citation

Chang, Sarah Y., and Feng-Yu Wang. "Determination of Gabapentin in Human Plasma By Capillary Electrophoresis With Laser-induced Fluorescence Detection and Acetonitrile Stacking Technique." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 799, no. 2, 2004, pp. 265-70.
Chang SY, Wang FY. Determination of gabapentin in human plasma by capillary electrophoresis with laser-induced fluorescence detection and acetonitrile stacking technique. J Chromatogr B Analyt Technol Biomed Life Sci. 2004;799(2):265-70.
Chang, S. Y., & Wang, F. Y. (2004). Determination of gabapentin in human plasma by capillary electrophoresis with laser-induced fluorescence detection and acetonitrile stacking technique. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 799(2), 265-70.
Chang SY, Wang FY. Determination of Gabapentin in Human Plasma By Capillary Electrophoresis With Laser-induced Fluorescence Detection and Acetonitrile Stacking Technique. J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jan 25;799(2):265-70. PubMed PMID: 14670745.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of gabapentin in human plasma by capillary electrophoresis with laser-induced fluorescence detection and acetonitrile stacking technique. AU - Chang,Sarah Y, AU - Wang,Feng-Yu, PY - 2003/12/13/pubmed PY - 2004/4/16/medline PY - 2003/12/13/entrez SP - 265 EP - 70 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 799 IS - 2 N2 - A sensitive analytical method for gabapentin [1-(aminomethyl) cyclohexaneacetic acid] (GBP) in human plasma based on capillary electrophoretic separation and laser-induced fluorescence (LIF) detection has been developed. 6-Carboxyfluorescein succinimidyl ester (CFSE) was used for precolumn derivatization of the non-fluorescent drug in plasma. Optimal separation and detection were obtained with an electrophoretic buffer of 50mM sodium borate (pH 9.5) and an air-cooled argon-ion laser (excitation at 488 nm, emission at 520 nm). A calibration curve ranging from 0.3 to 150 microM was shown to be linear. The concentration limit of detection (LOD) in plasma was 60 nM. We also demonstrate how the detection limit can be enhanced by using acetonitrile stacking technique. With stacking, the limit of detection for gabapentin in plasma was 4.8 nM. A calibration curve ranging from 0.03 to 15 microM was shown to be linear. Both the within-day and day-to-day reproducibility and accuracy were </=10.8 and 6.0%, respectively. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/14670745/Determination_of_gabapentin_in_human_plasma_by_capillary_electrophoresis_with_laser_induced_fluorescence_detection_and_acetonitrile_stacking_technique_ DB - PRIME DP - Unbound Medicine ER -