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Demonstration of a role for insulin in the regulation of leptin in lactating dairy cows.
J Dairy Sci 2003; 86(11):3508-15JD

Abstract

In lactating dairy cows, the onset of negative net energy balance (EB) at parturition causes a reduction in plasma leptin and is also associated with increased concentration of growth hormone (GH) and decreased concentration of insulin. These observations raise the possibility that opposite changes in plasma insulin and GH are partly responsible for reduced plasma leptin. To test this hypothesis, we first examined the effects of undernutrition without the confounding influence of parturition by using late lactating dairy cows fed 120% of their nutrient requirements or restricted to 33% of maintenance requirements. Plasma leptin was reduced within 24 h of feed restriction, and was associated with increased plasma GH and decreased plasma insulin. Complete food deprivation for 48 h caused similar changes in the plasma concentration of leptin. To determine if an elevation in GH is responsible for the fall in plasma leptin, dairy cows were treated with excipient or bovine somatotropin in early lactation or in late lactation. Growth hormone treatment had no significant effect on plasma leptin irrespective of stage of lactation. Finally, the effects of insulin were studied by performing euglycemic hyperinsulinemic clamps in mid-lactating dairy cows. After 96 h of hyperinsulinemia, plasma leptin was increased significantly. These data indicate that insulin regulates plasma leptin in lactating dairy cows. They also suggest that, in undernourished lactating dairy cows, reduced plasma insulin could account for a portion of the decline in plasma leptin but that elevated plasma GH is unlikely to have a major effect.

Authors+Show Affiliations

Department of Animal Science, Cornell University, Ithaca, NY 14853, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

14672180

Citation

Block, S S., et al. "Demonstration of a Role for Insulin in the Regulation of Leptin in Lactating Dairy Cows." Journal of Dairy Science, vol. 86, no. 11, 2003, pp. 3508-15.
Block SS, Rhoads RP, Bauman DE, et al. Demonstration of a role for insulin in the regulation of leptin in lactating dairy cows. J Dairy Sci. 2003;86(11):3508-15.
Block, S. S., Rhoads, R. P., Bauman, D. E., Ehrhardt, R. A., McGuire, M. A., Crooker, B. A., ... Boisclair, Y. R. (2003). Demonstration of a role for insulin in the regulation of leptin in lactating dairy cows. Journal of Dairy Science, 86(11), pp. 3508-15.
Block SS, et al. Demonstration of a Role for Insulin in the Regulation of Leptin in Lactating Dairy Cows. J Dairy Sci. 2003;86(11):3508-15. PubMed PMID: 14672180.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Demonstration of a role for insulin in the regulation of leptin in lactating dairy cows. AU - Block,S S, AU - Rhoads,R P, AU - Bauman,D E, AU - Ehrhardt,R A, AU - McGuire,M A, AU - Crooker,B A, AU - Griinari,J M, AU - Mackle,T R, AU - Weber,W J, AU - Van Amburgh,M E, AU - Boisclair,Y R, PY - 2003/12/16/pubmed PY - 2004/3/17/medline PY - 2003/12/16/entrez SP - 3508 EP - 15 JF - Journal of dairy science JO - J. Dairy Sci. VL - 86 IS - 11 N2 - In lactating dairy cows, the onset of negative net energy balance (EB) at parturition causes a reduction in plasma leptin and is also associated with increased concentration of growth hormone (GH) and decreased concentration of insulin. These observations raise the possibility that opposite changes in plasma insulin and GH are partly responsible for reduced plasma leptin. To test this hypothesis, we first examined the effects of undernutrition without the confounding influence of parturition by using late lactating dairy cows fed 120% of their nutrient requirements or restricted to 33% of maintenance requirements. Plasma leptin was reduced within 24 h of feed restriction, and was associated with increased plasma GH and decreased plasma insulin. Complete food deprivation for 48 h caused similar changes in the plasma concentration of leptin. To determine if an elevation in GH is responsible for the fall in plasma leptin, dairy cows were treated with excipient or bovine somatotropin in early lactation or in late lactation. Growth hormone treatment had no significant effect on plasma leptin irrespective of stage of lactation. Finally, the effects of insulin were studied by performing euglycemic hyperinsulinemic clamps in mid-lactating dairy cows. After 96 h of hyperinsulinemia, plasma leptin was increased significantly. These data indicate that insulin regulates plasma leptin in lactating dairy cows. They also suggest that, in undernourished lactating dairy cows, reduced plasma insulin could account for a portion of the decline in plasma leptin but that elevated plasma GH is unlikely to have a major effect. SN - 0022-0302 UR - https://www.unboundmedicine.com/medline/citation/14672180/Demonstration_of_a_role_for_insulin_in_the_regulation_of_leptin_in_lactating_dairy_cows_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-0302(03)73955-1 DB - PRIME DP - Unbound Medicine ER -