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Antioxidant and anticancer activity of extract from Betula platyphylla var. japonica.
Life Sci. 2004 Jan 09; 74(8):1013-26.LS

Abstract

The antioxidant and anticancer properties of a medicinal plant, Betula platyphylla var. japonica were investigated. The total methanol extract of B. platyphylla var. japonica had protective effects against hydrogen peroxide (H2O2) in the Chinese hamster lung fibroblast (V79-4) cell line and induced apoptotic cell death in human promyelocytic leukemia (HL-60) cells, a cancer cell line. B. platyphylla var. japonica extract significantly increased cell viability against H2O2. The extract also showed high 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (IC50 2.4 microg/ml) and lipid peroxidation inhibitory activity (IC50 below 4.0 microg/ml). Furthermore, B. platyphylla var. japonica extract reduced the number of V79-4 cells arrested in G2/M in response to H2O2 treatment and increased the activities of several cellular antioxidant enzymes, including superoxide dismutase, catalase and glutathione peroxidase. Treatment with B. platyphylla var. japonica extract induced cytotoxicity and apoptosis in HL-60 cells, as shown by nucleosomal DNA fragmentation, increases in the subdiploid cell population, and fluorescence microscopy. B. platyphylla var. japonica extract gradually increased the expression of pro-apoptotic Bax and led to the activation of caspase-3 and cleavage of PARP. These findings suggest that B. platyphylla var. japonica exhibits potential antioxidant and anticancer properties.

Authors+Show Affiliations

Department of Biochemistry, College of Dentistry, Kyung Hee University, 1 Hoeki-Dong, Dongdaemoon-Ku, Seoul 130-701, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14672757

Citation

Ju, Eun Mi, et al. "Antioxidant and Anticancer Activity of Extract From Betula Platyphylla Var. Japonica." Life Sciences, vol. 74, no. 8, 2004, pp. 1013-26.
Ju EM, Lee SE, Hwang HJ, et al. Antioxidant and anticancer activity of extract from Betula platyphylla var. japonica. Life Sci. 2004;74(8):1013-26.
Ju, E. M., Lee, S. E., Hwang, H. J., & Kim, J. H. (2004). Antioxidant and anticancer activity of extract from Betula platyphylla var. japonica. Life Sciences, 74(8), 1013-26.
Ju EM, et al. Antioxidant and Anticancer Activity of Extract From Betula Platyphylla Var. Japonica. Life Sci. 2004 Jan 9;74(8):1013-26. PubMed PMID: 14672757.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant and anticancer activity of extract from Betula platyphylla var. japonica. AU - Ju,Eun Mi, AU - Lee,Si Eun, AU - Hwang,Hyun Jin, AU - Kim,Jeong Hee, PY - 2003/12/16/pubmed PY - 2004/2/19/medline PY - 2003/12/16/entrez SP - 1013 EP - 26 JF - Life sciences JO - Life Sci VL - 74 IS - 8 N2 - The antioxidant and anticancer properties of a medicinal plant, Betula platyphylla var. japonica were investigated. The total methanol extract of B. platyphylla var. japonica had protective effects against hydrogen peroxide (H2O2) in the Chinese hamster lung fibroblast (V79-4) cell line and induced apoptotic cell death in human promyelocytic leukemia (HL-60) cells, a cancer cell line. B. platyphylla var. japonica extract significantly increased cell viability against H2O2. The extract also showed high 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (IC50 2.4 microg/ml) and lipid peroxidation inhibitory activity (IC50 below 4.0 microg/ml). Furthermore, B. platyphylla var. japonica extract reduced the number of V79-4 cells arrested in G2/M in response to H2O2 treatment and increased the activities of several cellular antioxidant enzymes, including superoxide dismutase, catalase and glutathione peroxidase. Treatment with B. platyphylla var. japonica extract induced cytotoxicity and apoptosis in HL-60 cells, as shown by nucleosomal DNA fragmentation, increases in the subdiploid cell population, and fluorescence microscopy. B. platyphylla var. japonica extract gradually increased the expression of pro-apoptotic Bax and led to the activation of caspase-3 and cleavage of PARP. These findings suggest that B. platyphylla var. japonica exhibits potential antioxidant and anticancer properties. SN - 0024-3205 UR - https://www.unboundmedicine.com/medline/citation/14672757/Antioxidant_and_anticancer_activity_of_extract_from_Betula_platyphylla_var__japonica_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024320503009743 DB - PRIME DP - Unbound Medicine ER -