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A genetic linkage map of the Durum x Triticum dicoccoides backcross population based on SSRs and AFLP markers, and QTL analysis for milling traits.
Theor Appl Genet. 2004 Feb; 108(3):401-13.TA

Abstract

Durum wheat (Triticum turgidum L. var durum) is mainly produced and consumed in the Mediterranean region; it is used to produce several specific end-products; such as local pasta, couscous and burghul. To study the genetics of grain-milling quality traits, chromosomal locations, and interaction with the environment, a genetic linkage map of durum was constructed and the quantitative trait loci QTLs for the milling-related traits, test weight (TW) and thousand-kernel weight (TKW), were identified. The population constituted 114 recombinant inbred lines derived from the cross: Omrabi 5 /Triticum dicoccoides 600545// Omrabi 5. TW and TKW were analyzed over 18 environments (sites x years). Single-sequence-repeat markers (SSRs), Amplified-fragment-length-polymorphism markers (AFLPs), and seed storage proteins (SSPs) showed a high level of polymorphism (>60%). The map was constructed with 124 SSRs, 149 AFLPs and 6 SSPs; its length covered 2,288.8 cM (8.2 cM/marker). The map showed high synteny with previous wheat maps, and both SSRs and AFLPs mapped evenly across the genome, with more markers in the B genome. However, some rearrangements were observed. For TW, a high genotypic effect was detected and two QTLs with epistasic effect were identified on 7AS and 6BS, explaining 30% of the total variation. The TKW showed a significant transgressive inheritance and five QTLs were identified, explaining 32% of the total variation, out of which 25% was of a genetic nature, and showing QTLxE interaction. The major TKW-QTLs were around the centromere region of 6B. For both traits, Omrabi 5 alleles had a significant positive effect. This population will be used to determine other QTLs of interest, as its parents are likely to harbor different genes for diseases and drought tolerance.

Authors+Show Affiliations

Elouafi I
Durum Improvement Program, ICARDA, P.O. Box 5466, Aleppo, Syria.
Nachit MM
No affiliation info available

MeSH

AllelesChromosome MappingGliadinGlutensHybridization, GeneticMicrosatellite RepeatsPhenotypePolymorphism, GeneticPolymorphism, Restriction Fragment LengthQuantitative Trait LociTriticum

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14676946

Citation

Elouafi, I, and M M. Nachit. "A Genetic Linkage Map of the Durum X Triticum Dicoccoides Backcross Population Based On SSRs and AFLP Markers, and QTL Analysis for Milling Traits." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 108, no. 3, 2004, pp. 401-13.
Elouafi I, Nachit MM. A genetic linkage map of the Durum x Triticum dicoccoides backcross population based on SSRs and AFLP markers, and QTL analysis for milling traits. Theor Appl Genet. 2004;108(3):401-13.
Elouafi, I., & Nachit, M. M. (2004). A genetic linkage map of the Durum x Triticum dicoccoides backcross population based on SSRs and AFLP markers, and QTL analysis for milling traits. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 108(3), 401-13.
Elouafi I, Nachit MM. A Genetic Linkage Map of the Durum X Triticum Dicoccoides Backcross Population Based On SSRs and AFLP Markers, and QTL Analysis for Milling Traits. Theor Appl Genet. 2004;108(3):401-13. PubMed PMID: 14676946.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A genetic linkage map of the Durum x Triticum dicoccoides backcross population based on SSRs and AFLP markers, and QTL analysis for milling traits. AU - Elouafi,I, AU - Nachit,M M, Y1 - 2003/12/16/ PY - 2003/01/20/received PY - 2003/08/12/accepted PY - 2003/12/17/pubmed PY - 2004/4/9/medline PY - 2003/12/17/entrez SP - 401 EP - 13 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 108 IS - 3 N2 - Durum wheat (Triticum turgidum L. var durum) is mainly produced and consumed in the Mediterranean region; it is used to produce several specific end-products; such as local pasta, couscous and burghul. To study the genetics of grain-milling quality traits, chromosomal locations, and interaction with the environment, a genetic linkage map of durum was constructed and the quantitative trait loci QTLs for the milling-related traits, test weight (TW) and thousand-kernel weight (TKW), were identified. The population constituted 114 recombinant inbred lines derived from the cross: Omrabi 5 /Triticum dicoccoides 600545// Omrabi 5. TW and TKW were analyzed over 18 environments (sites x years). Single-sequence-repeat markers (SSRs), Amplified-fragment-length-polymorphism markers (AFLPs), and seed storage proteins (SSPs) showed a high level of polymorphism (>60%). The map was constructed with 124 SSRs, 149 AFLPs and 6 SSPs; its length covered 2,288.8 cM (8.2 cM/marker). The map showed high synteny with previous wheat maps, and both SSRs and AFLPs mapped evenly across the genome, with more markers in the B genome. However, some rearrangements were observed. For TW, a high genotypic effect was detected and two QTLs with epistasic effect were identified on 7AS and 6BS, explaining 30% of the total variation. The TKW showed a significant transgressive inheritance and five QTLs were identified, explaining 32% of the total variation, out of which 25% was of a genetic nature, and showing QTLxE interaction. The major TKW-QTLs were around the centromere region of 6B. For both traits, Omrabi 5 alleles had a significant positive effect. This population will be used to determine other QTLs of interest, as its parents are likely to harbor different genes for diseases and drought tolerance. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/14676946/A_genetic_linkage_map_of_the_Durum_x_Triticum_dicoccoides_backcross_population_based_on_SSRs_and_AFLP_markers_and_QTL_analysis_for_milling_traits_ DB - PRIME DP - Unbound Medicine ER -