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Vesicular glutamate transporter 2 is expressed in different nerve fibre populations that selectively contact pulmonary neuroepithelial bodies.
Histochem Cell Biol 2004; 121(1):1-12HC

Abstract

Pulmonary neuroepithelial body (NEB) receptors in rats receive at least four different nerve fibre populations. In addition to a spinal sensory innervation that contacts NEBs at their basal side, extensive vagal nodose sensory terminals and separate nitrergic and cholinergic nerve endings protrude between NEB cells. In the present study, antibodies against the vesicular glutamate transporter 2 (VGLUT2), a transmembrane protein responsible for loading glutamate into synaptic vesicles, were used to investigate whether some of the nerve terminals contacting NEBs in rat lungs might use glutamate as a neurotransmitter. VGLUT2 immunoreactivity (IR) was detected in extensive intraepithelial arborising nerve terminals that appeared to contact most of the NEBs. Multiple immunostaining showed VGLUT2 IR in the vagal nodose and spinal sensory nerve terminals contacting NEBs, and in another, most likely sensory, intraepithelial nerve fibre population, the origin and further characteristics of which remain to be elucidated. At least part of the VGLUT2-immunoreactive nerve fibres that contact NEBs were shown to be myelinated. The expression of VGLUT2 indicates that glutamate is stored and released as a neurotransmitter in terminals of several pulmonary (sensory) nerve fibre populations that selectively relate to the complex NEB receptors. The present study strongly suggests an involvement of glutamatergic mechanisms in the peripheral transduction of sensory stimuli from the lungs, via the release of glutamate from nerve terminals, thereby modulating the activity of NEB receptor cells or the excitability of afferent nerves.

Authors+Show Affiliations

Laboratory of Cell Biology and Histology, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14677070

Citation

Brouns, Inge, et al. "Vesicular Glutamate Transporter 2 Is Expressed in Different Nerve Fibre Populations That Selectively Contact Pulmonary Neuroepithelial Bodies." Histochemistry and Cell Biology, vol. 121, no. 1, 2004, pp. 1-12.
Brouns I, Pintelon I, Van Genechten J, et al. Vesicular glutamate transporter 2 is expressed in different nerve fibre populations that selectively contact pulmonary neuroepithelial bodies. Histochem Cell Biol. 2004;121(1):1-12.
Brouns, I., Pintelon, I., Van Genechten, J., De Proost, I., Timmermans, J. P., & Adriaensen, D. (2004). Vesicular glutamate transporter 2 is expressed in different nerve fibre populations that selectively contact pulmonary neuroepithelial bodies. Histochemistry and Cell Biology, 121(1), pp. 1-12.
Brouns I, et al. Vesicular Glutamate Transporter 2 Is Expressed in Different Nerve Fibre Populations That Selectively Contact Pulmonary Neuroepithelial Bodies. Histochem Cell Biol. 2004;121(1):1-12. PubMed PMID: 14677070.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vesicular glutamate transporter 2 is expressed in different nerve fibre populations that selectively contact pulmonary neuroepithelial bodies. AU - Brouns,Inge, AU - Pintelon,Isabel, AU - Van Genechten,Jeroen, AU - De Proost,Ian, AU - Timmermans,Jean-Pierre, AU - Adriaensen,Dirk, Y1 - 2003/12/16/ PY - 2003/11/19/accepted PY - 2003/12/17/pubmed PY - 2004/9/15/medline PY - 2003/12/17/entrez SP - 1 EP - 12 JF - Histochemistry and cell biology JO - Histochem. Cell Biol. VL - 121 IS - 1 N2 - Pulmonary neuroepithelial body (NEB) receptors in rats receive at least four different nerve fibre populations. In addition to a spinal sensory innervation that contacts NEBs at their basal side, extensive vagal nodose sensory terminals and separate nitrergic and cholinergic nerve endings protrude between NEB cells. In the present study, antibodies against the vesicular glutamate transporter 2 (VGLUT2), a transmembrane protein responsible for loading glutamate into synaptic vesicles, were used to investigate whether some of the nerve terminals contacting NEBs in rat lungs might use glutamate as a neurotransmitter. VGLUT2 immunoreactivity (IR) was detected in extensive intraepithelial arborising nerve terminals that appeared to contact most of the NEBs. Multiple immunostaining showed VGLUT2 IR in the vagal nodose and spinal sensory nerve terminals contacting NEBs, and in another, most likely sensory, intraepithelial nerve fibre population, the origin and further characteristics of which remain to be elucidated. At least part of the VGLUT2-immunoreactive nerve fibres that contact NEBs were shown to be myelinated. The expression of VGLUT2 indicates that glutamate is stored and released as a neurotransmitter in terminals of several pulmonary (sensory) nerve fibre populations that selectively relate to the complex NEB receptors. The present study strongly suggests an involvement of glutamatergic mechanisms in the peripheral transduction of sensory stimuli from the lungs, via the release of glutamate from nerve terminals, thereby modulating the activity of NEB receptor cells or the excitability of afferent nerves. SN - 0948-6143 UR - https://www.unboundmedicine.com/medline/citation/14677070/Vesicular_glutamate_transporter_2_is_expressed_in_different_nerve_fibre_populations_that_selectively_contact_pulmonary_neuroepithelial_bodies_ L2 - https://dx.doi.org/10.1007/s00418-003-0609-1 DB - PRIME DP - Unbound Medicine ER -