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A functional anti-human 4-1BB ligand monoclonal antibody that enhances proliferation of monocytes by reverse signaling of 4-1BBL.
Hybrid Hybridomics. 2003 Oct; 22(5):333-8.HH

Abstract

4-1BB Ligand (4-1BBL), a transmembrane molecule, member of the tumor necrosis factor ligand superfamily, is an important costimulatory molecule in the immune response. In this study a functional anti-human 4-1BBL MAb 1F1 was obtained and the specificity of this MAb was verified by flow cytometry and Western blotting. This MAb effectively recognized the 4-1BBL molecule expressed on a series of malignant cell lines as well as on DC and monocytes and it inhibited the proliferation of T lymphocytes, costimulated by soluble 4-1BBL and agonist anti-human CD3 MAb. Furthermore, we demonstrated that MAb 1F1 induced an impressive proliferation of monocytes from peripheral blood by triggering the reverse signal through 4-1BBL. This functional anti-human 4-1BBL MAb provides a valuable tool for further study of biological functions as well as signal transduction of 4-1BBL/4-1BB.

Links

clones/clone libraries

Authors+Show Affiliations

Ju SW
Biotechnology Institute, Soochow University, Suzhou, China.
Ju SG
No affiliation info available
Wang FM
No affiliation info available
Gu ZJ
No affiliation info available
Qiu YH
No affiliation info available
Yu GH
No affiliation info available
Ma HB
No affiliation info available
Zhang XG
No affiliation info available

MeSH

4-1BB LigandAnimalsAntibodies, MonoclonalAntigens, CDCell LineHumansImmunoglobulin GMiceMice, Inbred BALB CMonocytesReceptors, Nerve Growth FactorReceptors, Tumor Necrosis FactorT-LymphocytesTumor Cells, CulturedTumor Necrosis Factor Receptor Superfamily, Member 9Tumor Necrosis Factor-alpha

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14678652

Citation

Ju, Song-Wen, et al. "A Functional Anti-human 4-1BB Ligand Monoclonal Antibody That Enhances Proliferation of Monocytes By Reverse Signaling of 4-1BBL." Hybridoma and Hybridomics, vol. 22, no. 5, 2003, pp. 333-8.
Ju SW, Ju SG, Wang FM, et al. A functional anti-human 4-1BB ligand monoclonal antibody that enhances proliferation of monocytes by reverse signaling of 4-1BBL. Hybrid Hybridomics. 2003;22(5):333-8.
Ju, S. W., Ju, S. G., Wang, F. M., Gu, Z. J., Qiu, Y. H., Yu, G. H., Ma, H. B., & Zhang, X. G. (2003). A functional anti-human 4-1BB ligand monoclonal antibody that enhances proliferation of monocytes by reverse signaling of 4-1BBL. Hybridoma and Hybridomics, 22(5), 333-8.
Ju SW, et al. A Functional Anti-human 4-1BB Ligand Monoclonal Antibody That Enhances Proliferation of Monocytes By Reverse Signaling of 4-1BBL. Hybrid Hybridomics. 2003;22(5):333-8. PubMed PMID: 14678652.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A functional anti-human 4-1BB ligand monoclonal antibody that enhances proliferation of monocytes by reverse signaling of 4-1BBL. AU - Ju,Song-Wen, AU - Ju,Song-Guang, AU - Wang,Feng-Ming, AU - Gu,Zong-Jiang, AU - Qiu,Yu-Hua, AU - Yu,Ge-Hua, AU - Ma,Hong-Bing, AU - Zhang,Xue-Guang, PY - 2003/12/18/pubmed PY - 2004/7/16/medline PY - 2003/12/18/entrez SP - 333 EP - 8 JF - Hybridoma and hybridomics JO - Hybrid Hybridomics VL - 22 IS - 5 N2 - 4-1BB Ligand (4-1BBL), a transmembrane molecule, member of the tumor necrosis factor ligand superfamily, is an important costimulatory molecule in the immune response. In this study a functional anti-human 4-1BBL MAb 1F1 was obtained and the specificity of this MAb was verified by flow cytometry and Western blotting. This MAb effectively recognized the 4-1BBL molecule expressed on a series of malignant cell lines as well as on DC and monocytes and it inhibited the proliferation of T lymphocytes, costimulated by soluble 4-1BBL and agonist anti-human CD3 MAb. Furthermore, we demonstrated that MAb 1F1 induced an impressive proliferation of monocytes from peripheral blood by triggering the reverse signal through 4-1BBL. This functional anti-human 4-1BBL MAb provides a valuable tool for further study of biological functions as well as signal transduction of 4-1BBL/4-1BB. SN - 1536-8599 UR - https://www.unboundmedicine.com/medline/citation/14678652/A_functional_anti_human_4_1BB_ligand_monoclonal_antibody_that_enhances_proliferation_of_monocytes_by_reverse_signaling_of_4_1BBL_ L2 - https://antibodies.cancer.gov/detail/CPTC-TNFRSF9-1 DB - PRIME DP - Unbound Medicine ER -