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Effects of the interaction between the C677T 5,10-methylenetetrahydrofolate reductase polymorphism and serum B vitamins on homocysteine levels in pregnant women.
Eur J Clin Nutr. 2004 Jan; 58(1):10-6.EJ

Abstract

OBJECTIVE

The purpose of this study was to investigate the effect of the interaction between the C677T mutation in the 5,10-methylenetetrahydrofolate reductase (MTHFR) genotypes and serum levels of B vitamins on serum homocysteine levels in pregnant women.

DESIGN

A cross-sectional study.

SETTING

Ewha Womans University Hospital, Seoul, Korea.

SUBJECTS

A total of 177 normal pregnant women, 24.6+/-1.1 weeks of gestation, in a 6-month period during 2001-2002.

INTERVENTIONS

Serum vitamin B2, vitamin B6, and homocysteine analyses were conducted using high-performance liquid chromatography methods. Serum folate and vitamin B12 concentrations were determined using a radioimmunoassay kit. MTHFR gene mutation was investigated by the polymerase chain reaction of a genomic DNA fragment.

RESULTS

Serum homocysteine was higher in women with the T/T genotype than those with the C/T or C/C genotype of the MTHFR gene (P<0.05). Serum homocysteine was negatively correlated with serum folate in all MTHFR genotypes (P<0.001), and the correlation between the two serum levels was the strongest in the T/T genotype. Serum homocysteine was higher in the subjects with the T/T MTHFR genotype only when the serum folate was below the median level. Explanatory power of B vitamin status as predictors of serum homocysteine levels was more pronounced in the T/T genotypes (68.5%) compared with the C/T (37.9%) or C/C genotypes (20.6%).

CONCLUSIONS

Serum homocysteine levels in pregnant women varied significantly with MTHFR genotype and the serum B vitamin status. Higher serum folate, vitamin B2, and vitamin B12 concentrations may lessen the MTHFR genotypic effect on serum homocysteine levels.

Authors+Show Affiliations

Department of Food and Nutritional Sciences, Ewha Womans University, Seoul, Korea.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14679361

Citation

Kim, K N., et al. "Effects of the Interaction Between the C677T 5,10-methylenetetrahydrofolate Reductase Polymorphism and Serum B Vitamins On Homocysteine Levels in Pregnant Women." European Journal of Clinical Nutrition, vol. 58, no. 1, 2004, pp. 10-6.
Kim KN, Kim YJ, Chang N. Effects of the interaction between the C677T 5,10-methylenetetrahydrofolate reductase polymorphism and serum B vitamins on homocysteine levels in pregnant women. Eur J Clin Nutr. 2004;58(1):10-6.
Kim, K. N., Kim, Y. J., & Chang, N. (2004). Effects of the interaction between the C677T 5,10-methylenetetrahydrofolate reductase polymorphism and serum B vitamins on homocysteine levels in pregnant women. European Journal of Clinical Nutrition, 58(1), 10-6.
Kim KN, Kim YJ, Chang N. Effects of the Interaction Between the C677T 5,10-methylenetetrahydrofolate Reductase Polymorphism and Serum B Vitamins On Homocysteine Levels in Pregnant Women. Eur J Clin Nutr. 2004;58(1):10-6. PubMed PMID: 14679361.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of the interaction between the C677T 5,10-methylenetetrahydrofolate reductase polymorphism and serum B vitamins on homocysteine levels in pregnant women. AU - Kim,K N, AU - Kim,Y J, AU - Chang,N, PY - 2003/12/18/pubmed PY - 2004/6/4/medline PY - 2003/12/18/entrez SP - 10 EP - 6 JF - European journal of clinical nutrition JO - Eur J Clin Nutr VL - 58 IS - 1 N2 - OBJECTIVE: The purpose of this study was to investigate the effect of the interaction between the C677T mutation in the 5,10-methylenetetrahydrofolate reductase (MTHFR) genotypes and serum levels of B vitamins on serum homocysteine levels in pregnant women. DESIGN: A cross-sectional study. SETTING: Ewha Womans University Hospital, Seoul, Korea. SUBJECTS: A total of 177 normal pregnant women, 24.6+/-1.1 weeks of gestation, in a 6-month period during 2001-2002. INTERVENTIONS: Serum vitamin B2, vitamin B6, and homocysteine analyses were conducted using high-performance liquid chromatography methods. Serum folate and vitamin B12 concentrations were determined using a radioimmunoassay kit. MTHFR gene mutation was investigated by the polymerase chain reaction of a genomic DNA fragment. RESULTS: Serum homocysteine was higher in women with the T/T genotype than those with the C/T or C/C genotype of the MTHFR gene (P<0.05). Serum homocysteine was negatively correlated with serum folate in all MTHFR genotypes (P<0.001), and the correlation between the two serum levels was the strongest in the T/T genotype. Serum homocysteine was higher in the subjects with the T/T MTHFR genotype only when the serum folate was below the median level. Explanatory power of B vitamin status as predictors of serum homocysteine levels was more pronounced in the T/T genotypes (68.5%) compared with the C/T (37.9%) or C/C genotypes (20.6%). CONCLUSIONS: Serum homocysteine levels in pregnant women varied significantly with MTHFR genotype and the serum B vitamin status. Higher serum folate, vitamin B2, and vitamin B12 concentrations may lessen the MTHFR genotypic effect on serum homocysteine levels. SN - 0954-3007 UR - https://www.unboundmedicine.com/medline/citation/14679361/Effects_of_the_interaction_between_the_C677T_510_methylenetetrahydrofolate_reductase_polymorphism_and_serum_B_vitamins_on_homocysteine_levels_in_pregnant_women_ L2 - http://dx.doi.org/10.1038/sj.ejcn.1601729 DB - PRIME DP - Unbound Medicine ER -