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Blastomyces dermatitidis lysate antigens: antibody detection in serial serum specimens from dogs with blastomycosis.
Mycopathologia. 2003; 156(4):289-94.M

Abstract

Yeast phase lysate antigens prepared from different isolates of Blastomyces dermatitidis (T-58, dog-Tennessee; T-27, polar bear-Tennessee; ERC-2, dog-Wisconsin; ER-3, woodpile-Wisconsin) were compared with respect to the detection of antibodies (indirect enzyme-linked immunosorbent assay-ELISA, peroxidase system) in 126 serial serum specimens (pre-treatment, 30 and 60 days post-treatment with itraconazole) from 42 dogs with diagnosed blastomycosis. Mean absorbance values observed with the four lysate antigens at the three treatment intervals ranged from the most reactive to the least reactive as follows: T-58 (0.270, 0.210, 0.136); T-27 (0.209, 0.156, 0.096); ER-3 (0.189, 0.144, 0.089) and ERC-2 (0.158, 0.129, 0.080). Even though variations in reactivity were evidenced, the lysates prepared from isolates from various geographical regions and sources were all efficacious as antigens for the immunodiagnosis of canine blastomycosis.

Authors+Show Affiliations

Chester EM
Department of Biological Sciences, Idaho State University, Pocatello, Idaho 83209, USA.
Axtell RC
No affiliation info available
Scalarone GM
No affiliation info available

MeSH

AnimalsAntibodies, FungalAntigens, FungalBlastomycesBlastomycosisDog DiseasesDogsEnzyme-Linked Immunosorbent Assay

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14682453

Citation

Chester, E M., et al. "Blastomyces Dermatitidis Lysate Antigens: Antibody Detection in Serial Serum Specimens From Dogs With Blastomycosis." Mycopathologia, vol. 156, no. 4, 2003, pp. 289-94.
Chester EM, Axtell RC, Scalarone GM. Blastomyces dermatitidis lysate antigens: antibody detection in serial serum specimens from dogs with blastomycosis. Mycopathologia. 2003;156(4):289-94.
Chester, E. M., Axtell, R. C., & Scalarone, G. M. (2003). Blastomyces dermatitidis lysate antigens: antibody detection in serial serum specimens from dogs with blastomycosis. Mycopathologia, 156(4), 289-94.
Chester EM, Axtell RC, Scalarone GM. Blastomyces Dermatitidis Lysate Antigens: Antibody Detection in Serial Serum Specimens From Dogs With Blastomycosis. Mycopathologia. 2003;156(4):289-94. PubMed PMID: 14682453.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Blastomyces dermatitidis lysate antigens: antibody detection in serial serum specimens from dogs with blastomycosis. AU - Chester,E M, AU - Axtell,R C, AU - Scalarone,G M, PY - 2003/12/20/pubmed PY - 2004/3/10/medline PY - 2003/12/20/entrez SP - 289 EP - 94 JF - Mycopathologia JO - Mycopathologia VL - 156 IS - 4 N2 - Yeast phase lysate antigens prepared from different isolates of Blastomyces dermatitidis (T-58, dog-Tennessee; T-27, polar bear-Tennessee; ERC-2, dog-Wisconsin; ER-3, woodpile-Wisconsin) were compared with respect to the detection of antibodies (indirect enzyme-linked immunosorbent assay-ELISA, peroxidase system) in 126 serial serum specimens (pre-treatment, 30 and 60 days post-treatment with itraconazole) from 42 dogs with diagnosed blastomycosis. Mean absorbance values observed with the four lysate antigens at the three treatment intervals ranged from the most reactive to the least reactive as follows: T-58 (0.270, 0.210, 0.136); T-27 (0.209, 0.156, 0.096); ER-3 (0.189, 0.144, 0.089) and ERC-2 (0.158, 0.129, 0.080). Even though variations in reactivity were evidenced, the lysates prepared from isolates from various geographical regions and sources were all efficacious as antigens for the immunodiagnosis of canine blastomycosis. SN - 0301-486X UR - https://www.unboundmedicine.com/medline/citation/14682453/Blastomyces_dermatitidis_lysate_antigens:_antibody_detection_in_serial_serum_specimens_from_dogs_with_blastomycosis_ DB - PRIME DP - Unbound Medicine ER -