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Production of interleukin 3 and granulocyte-macrophage colony-stimulating factor from stimulated blood mononuclear cells in patients with aplastic anemia.
Exp Hematol. 1992 Oct; 20(9):1125-8.EH

Abstract

Blood cells from patients with aplastic anemia (AA) were evaluated for the ability to produce interleukin 3 (IL-3) and granulocyte-macrophage colony-stimulating factor (GM-CSF) on stimulation with phytohemagglutinin (PHA) or antilymphocyte globulin (ALG) by the use of an IL-3-dependent cell-line, TF-1, and the GM-CSF-IRMA kit. The IL-3 levels in patients with active AA were significantly lower, both in PHA-stimulated conditioned medium (CM) and in ALG-CM, than those of normal healthy donors (HD; p < 0.01). The degree of reduced IL-3 production in AA patients correlated well with the severity of neutropenia; the level of IL-3 returned to normal after successful treatment with ALG plus methylprednisolone (ALG therapy). On the other hand, GM-CSF production in AA patients varied widely and was only significant in remission patients in PHA-CM; in this case production was higher than that in active AA patients (p < 0.05) or in HD (p < 0.01). Sensitivity to PHA or ALG stimulation was evaluated by the ratio of IL-3 concentrations in ALG-CM versus PHA-CM (ALG/PHA index). The index varied widely from < 0.1 to > 10 in AA patients, contrasting to the clustered values in HD. Seven of the eight patients who had an ALG/PHA index of > 1.0 showed a good clinical response to ALG therapy. However, 12 of the 14 patients who had a lower index (< 1.0) failed to respond. The ALG/PHA index might have an ability to predict the response to ALG therapy.

Authors+Show Affiliations

Department of Pediatrics, University of Tokushima, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1468545

Citation

Kawano, Y, et al. "Production of Interleukin 3 and Granulocyte-macrophage Colony-stimulating Factor From Stimulated Blood Mononuclear Cells in Patients With Aplastic Anemia." Experimental Hematology, vol. 20, no. 9, 1992, pp. 1125-8.
Kawano Y, Takaue Y, Hirao A, et al. Production of interleukin 3 and granulocyte-macrophage colony-stimulating factor from stimulated blood mononuclear cells in patients with aplastic anemia. Exp Hematol. 1992;20(9):1125-8.
Kawano, Y., Takaue, Y., Hirao, A., Watanabe, T., Abe, T., Shimizu, T., Sato, J., Saito, S., Kitamura, T., & Takaku, F. (1992). Production of interleukin 3 and granulocyte-macrophage colony-stimulating factor from stimulated blood mononuclear cells in patients with aplastic anemia. Experimental Hematology, 20(9), 1125-8.
Kawano Y, et al. Production of Interleukin 3 and Granulocyte-macrophage Colony-stimulating Factor From Stimulated Blood Mononuclear Cells in Patients With Aplastic Anemia. Exp Hematol. 1992;20(9):1125-8. PubMed PMID: 1468545.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Production of interleukin 3 and granulocyte-macrophage colony-stimulating factor from stimulated blood mononuclear cells in patients with aplastic anemia. A1 - Kawano,Y, AU - Takaue,Y, AU - Hirao,A, AU - Watanabe,T, AU - Abe,T, AU - Shimizu,T, AU - Sato,J, AU - Saito,S, AU - Kitamura,T, AU - Takaku,F, PY - 1992/10/1/pubmed PY - 1992/10/1/medline PY - 1992/10/1/entrez SP - 1125 EP - 8 JF - Experimental hematology JO - Exp Hematol VL - 20 IS - 9 N2 - Blood cells from patients with aplastic anemia (AA) were evaluated for the ability to produce interleukin 3 (IL-3) and granulocyte-macrophage colony-stimulating factor (GM-CSF) on stimulation with phytohemagglutinin (PHA) or antilymphocyte globulin (ALG) by the use of an IL-3-dependent cell-line, TF-1, and the GM-CSF-IRMA kit. The IL-3 levels in patients with active AA were significantly lower, both in PHA-stimulated conditioned medium (CM) and in ALG-CM, than those of normal healthy donors (HD; p < 0.01). The degree of reduced IL-3 production in AA patients correlated well with the severity of neutropenia; the level of IL-3 returned to normal after successful treatment with ALG plus methylprednisolone (ALG therapy). On the other hand, GM-CSF production in AA patients varied widely and was only significant in remission patients in PHA-CM; in this case production was higher than that in active AA patients (p < 0.05) or in HD (p < 0.01). Sensitivity to PHA or ALG stimulation was evaluated by the ratio of IL-3 concentrations in ALG-CM versus PHA-CM (ALG/PHA index). The index varied widely from < 0.1 to > 10 in AA patients, contrasting to the clustered values in HD. Seven of the eight patients who had an ALG/PHA index of > 1.0 showed a good clinical response to ALG therapy. However, 12 of the 14 patients who had a lower index (< 1.0) failed to respond. The ALG/PHA index might have an ability to predict the response to ALG therapy. SN - 0301-472X UR - https://www.unboundmedicine.com/medline/citation/1468545/Production_of_interleukin_3_and_granulocyte_macrophage_colony_stimulating_factor_from_stimulated_blood_mononuclear_cells_in_patients_with_aplastic_anemia_ L2 - https://www.diseaseinfosearch.org/result/556 DB - PRIME DP - Unbound Medicine ER -