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B chromosomes of the Podisma sapporensis Shir. (Orthoptera, Acrididae) analysed by chromosome microdissection and FISH.
Folia Biol (Krakow). 2003; 51(1-2):1-11.FB

Abstract

The analysis of the distribution of repetitive DNA of the B chromosomes of Podisma sapporensis in the A and B chromosomes of the natural populations and in A chromosomes of three other species of the Podismini grasshoppers were made. DNA-libraries of the B chromosome and the euchromatic segment of the A chromosome of P. sapporensis were generated by meiotic chromosome microdissection followed by degenerated oligonucleotide primed polymerase chain reaction (DOP-PCR). Paints based on these DNA-libraries were used for FISH analysis to detect localization of homologous sequences in A and B chromosomes of P. sapporensis from different natural populations. On the basis of the FISH analysis the authors suggest that evolution of the B chromosomes in Podisma sapporensis was associated mainly with the insertions of "alien DNA sequences" into ancestral A chromosome and their further amplification. The number of initial sites of amplifications differed in the different Bs, the distance between these sites also varying. Karyotype evolution in P. sapporensis was associated partly with the insertion of "alien DNA sequences" into pericentromeric chromosomal regions. Insertion into the small short arms of the acrocentric chromosomes followed, with the DNA amplification leading to the formation of the additional C-heterochromatic arms or euchromatic-like regions of different size.

Authors+Show Affiliations

Novosibirsk State University, 630090 Novosibirsk, Russia. bugrov@fen.nsu.ruNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14686642

Citation

Bugrov, Alexander G., et al. "B Chromosomes of the Podisma Sapporensis Shir. (Orthoptera, Acrididae) Analysed By Chromosome Microdissection and FISH." Folia Biologica, vol. 51, no. 1-2, 2003, pp. 1-11.
Bugrov AG, Karamysheva TV, Pyatkova MS, et al. B chromosomes of the Podisma sapporensis Shir. (Orthoptera, Acrididae) analysed by chromosome microdissection and FISH. Folia Biol (Krakow). 2003;51(1-2):1-11.
Bugrov, A. G., Karamysheva, T. V., Pyatkova, M. S., Rubtsov, D. N., Andreenkova, O. V., Warchałowska-Sliwa, E., & Rubtsov, N. B. (2003). B chromosomes of the Podisma sapporensis Shir. (Orthoptera, Acrididae) analysed by chromosome microdissection and FISH. Folia Biologica, 51(1-2), 1-11.
Bugrov AG, et al. B Chromosomes of the Podisma Sapporensis Shir. (Orthoptera, Acrididae) Analysed By Chromosome Microdissection and FISH. Folia Biol (Krakow). 2003;51(1-2):1-11. PubMed PMID: 14686642.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - B chromosomes of the Podisma sapporensis Shir. (Orthoptera, Acrididae) analysed by chromosome microdissection and FISH. AU - Bugrov,Alexander G, AU - Karamysheva,Tatiana V, AU - Pyatkova,Maria S, AU - Rubtsov,Denis N, AU - Andreenkova,Olga V, AU - Warchałowska-Sliwa,Elzbieta, AU - Rubtsov,Nikolai B, PY - 2003/12/23/pubmed PY - 2004/1/7/medline PY - 2003/12/23/entrez SP - 1 EP - 11 JF - Folia biologica JO - Folia Biol. (Krakow) VL - 51 IS - 1-2 N2 - The analysis of the distribution of repetitive DNA of the B chromosomes of Podisma sapporensis in the A and B chromosomes of the natural populations and in A chromosomes of three other species of the Podismini grasshoppers were made. DNA-libraries of the B chromosome and the euchromatic segment of the A chromosome of P. sapporensis were generated by meiotic chromosome microdissection followed by degenerated oligonucleotide primed polymerase chain reaction (DOP-PCR). Paints based on these DNA-libraries were used for FISH analysis to detect localization of homologous sequences in A and B chromosomes of P. sapporensis from different natural populations. On the basis of the FISH analysis the authors suggest that evolution of the B chromosomes in Podisma sapporensis was associated mainly with the insertions of "alien DNA sequences" into ancestral A chromosome and their further amplification. The number of initial sites of amplifications differed in the different Bs, the distance between these sites also varying. Karyotype evolution in P. sapporensis was associated partly with the insertion of "alien DNA sequences" into pericentromeric chromosomal regions. Insertion into the small short arms of the acrocentric chromosomes followed, with the DNA amplification leading to the formation of the additional C-heterochromatic arms or euchromatic-like regions of different size. SN - 0015-5497 UR - https://www.unboundmedicine.com/medline/citation/14686642/B_chromosomes_of_the_Podisma_sapporensis_Shir___Orthoptera_Acrididae__analysed_by_chromosome_microdissection_and_FISH_ DB - PRIME DP - Unbound Medicine ER -