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Long-term effects of imidapril on calcium and potassium currents in rabbit left ventricular hypertrophic myocytes.
Chin Med J (Engl). 2003 Dec; 116(12):1795-8.CM

Abstract

OBJECTIVE

To investigate the long-term effects of imidapril (IMI) on action potential and calcium and potassium currents in rabbit left ventricular hypertrophic myocytes.

METHODS

Rabbits were randomly divided into three groups: IMI-treated, hypertrophic and sham-operated control groups. Cardiac hypertrophy was induced in hypertrophy group by partial ligation of the abdominal aorta. In the IMI-treated group, the rabbits were administered IMI (1.5 mg x kg(-1) x d(-1)) for 8 weeks after surgery. In the sham-operated control group, the animals underwent an abdominal laparotomy without further procedure. Whole-cell patch clamp technique was used to record ionic currents.

RESULTS

Membrane capacitance was larger in hypertrophic cells than in sham-operated cells or IMI-treated cells. Action potential duration was lengthened in hypertrophic cells and was remarkably shortened by IMI. The density of ICa, L was reduced from 12.8 +/- 0.7 pA/pF in the sham-operated cells, to 7.7 +/- 0.8 pA/pF in hypertrophic cells, while it resembled the control cells after IMI treatment (11.9 +/- 1.0 pA/pF). After IMI treatment, the density of I(Ks,tail) was enhanced from 2.5 +/- 0.1 pA/pF in hypertrophic cells to 4.7 +/- 0.6 pA/pF (n = 7, P < 0.01), which was similar to the sham-operated cells. The densities of Ito and IK1 were significantly increased in IMI-treated cells, from 3.8 +/- 0.4 pA/pF and 3.7 +/- 0.5 pA/pF in the hypertrophic cells to 6.4 +/- 0.8 pA/pF and 6.5 +/- 0.3 pA/pF, respectively, but the IKr densities were not different in the three groups.

CONCLUSION

IMI could reverse the increase in membrane capacitance in hypertrophic cells, shorten action potential duration, and increase the densities of ICa, L, IKs, Ito and IK1 in hypertrophic cells.

Authors+Show Affiliations

Department of Cardiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. liyang40@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14687462

Citation

Li, Yang, et al. "Long-term Effects of Imidapril On Calcium and Potassium Currents in Rabbit Left Ventricular Hypertrophic Myocytes." Chinese Medical Journal, vol. 116, no. 12, 2003, pp. 1795-8.
Li Y, Lu Z, Xiao J, et al. Long-term effects of imidapril on calcium and potassium currents in rabbit left ventricular hypertrophic myocytes. Chin Med J (Engl). 2003;116(12):1795-8.
Li, Y., Lu, Z., Xiao, J., Zhang, C., Ma, J., & Liu, N. (2003). Long-term effects of imidapril on calcium and potassium currents in rabbit left ventricular hypertrophic myocytes. Chinese Medical Journal, 116(12), 1795-8.
Li Y, et al. Long-term Effects of Imidapril On Calcium and Potassium Currents in Rabbit Left Ventricular Hypertrophic Myocytes. Chin Med J (Engl). 2003;116(12):1795-8. PubMed PMID: 14687462.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long-term effects of imidapril on calcium and potassium currents in rabbit left ventricular hypertrophic myocytes. AU - Li,Yang, AU - Lu,Zaiying, AU - Xiao,Jianmin, AU - Zhang,Cuntai, AU - Ma,Jie, AU - Liu,Nian, PY - 2003/12/23/pubmed PY - 2004/5/5/medline PY - 2003/12/23/entrez SP - 1795 EP - 8 JF - Chinese medical journal JO - Chin Med J (Engl) VL - 116 IS - 12 N2 - OBJECTIVE: To investigate the long-term effects of imidapril (IMI) on action potential and calcium and potassium currents in rabbit left ventricular hypertrophic myocytes. METHODS: Rabbits were randomly divided into three groups: IMI-treated, hypertrophic and sham-operated control groups. Cardiac hypertrophy was induced in hypertrophy group by partial ligation of the abdominal aorta. In the IMI-treated group, the rabbits were administered IMI (1.5 mg x kg(-1) x d(-1)) for 8 weeks after surgery. In the sham-operated control group, the animals underwent an abdominal laparotomy without further procedure. Whole-cell patch clamp technique was used to record ionic currents. RESULTS: Membrane capacitance was larger in hypertrophic cells than in sham-operated cells or IMI-treated cells. Action potential duration was lengthened in hypertrophic cells and was remarkably shortened by IMI. The density of ICa, L was reduced from 12.8 +/- 0.7 pA/pF in the sham-operated cells, to 7.7 +/- 0.8 pA/pF in hypertrophic cells, while it resembled the control cells after IMI treatment (11.9 +/- 1.0 pA/pF). After IMI treatment, the density of I(Ks,tail) was enhanced from 2.5 +/- 0.1 pA/pF in hypertrophic cells to 4.7 +/- 0.6 pA/pF (n = 7, P < 0.01), which was similar to the sham-operated cells. The densities of Ito and IK1 were significantly increased in IMI-treated cells, from 3.8 +/- 0.4 pA/pF and 3.7 +/- 0.5 pA/pF in the hypertrophic cells to 6.4 +/- 0.8 pA/pF and 6.5 +/- 0.3 pA/pF, respectively, but the IKr densities were not different in the three groups. CONCLUSION: IMI could reverse the increase in membrane capacitance in hypertrophic cells, shorten action potential duration, and increase the densities of ICa, L, IKs, Ito and IK1 in hypertrophic cells. SN - 0366-6999 UR - https://www.unboundmedicine.com/medline/citation/14687462/Long_term_effects_of_imidapril_on_calcium_and_potassium_currents_in_rabbit_left_ventricular_hypertrophic_myocytes_ DB - PRIME DP - Unbound Medicine ER -