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Evaluation of Kluyveromyces marxianus FII 510700 grown on a lactose-based medium as a source of a natural bioemulsifier.
J Ind Microbiol Biotechnol. 2003 Dec; 30(12):715-20.JI

Abstract

Mannoprotein with emulsification properties was extracted from the cell walls of Kluyveromyces marxianus grown on a lactose-based medium by autoclaving cells in a citrate buffer at pH 7. The purified product was evaluated for chemical and physical stability to establish its potential use as a natural emulsifier in processed foods. The yield of purified bioemulsifier from this strain of K. marxianus was 4-7% of the original dry cell weight. The purified product, at a concentration of 12 g l(-1), formed emulsions that were stable for 3 months when subjected to a range of pH (3-11) and NaCl concentrations (2-50 g l(-1)). The composition of this mannoprotein was 90% carbohydrate (mannan) and 4-6% protein. These values are similar to mannoprotein extracted from cells of Saccharomyces cerevisiae, which is the traditional source. Consequently K. marxianus cultivated on a low-cost lactose-based medium such as whey, a lactose-rich clean waste of the dairy industry, could be developed as a source of bioemulsifier for use in the food industry.

Authors+Show Affiliations

Lukondeh T
School of Civil and Environmental Engineering, The University of New South Wales, 2052 Sydney, Australia. t.lukondeh@unsw.edu.au
Ashbolt NJ
No affiliation info available
Rogers PL
No affiliation info available

MeSH

Carbohydrate MetabolismCulture MediaEmulsifying AgentsFood IndustryHydrogen-Ion ConcentrationIndustrial MicrobiologyKluyveromycesLactoseMilk ProteinsMolecular WeightProteinsSodium ChlorideWhey Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14689315

Citation

Lukondeh, Tredwell, et al. "Evaluation of Kluyveromyces Marxianus FII 510700 Grown On a Lactose-based Medium as a Source of a Natural Bioemulsifier." Journal of Industrial Microbiology & Biotechnology, vol. 30, no. 12, 2003, pp. 715-20.
Lukondeh T, Ashbolt NJ, Rogers PL. Evaluation of Kluyveromyces marxianus FII 510700 grown on a lactose-based medium as a source of a natural bioemulsifier. J Ind Microbiol Biotechnol. 2003;30(12):715-20.
Lukondeh, T., Ashbolt, N. J., & Rogers, P. L. (2003). Evaluation of Kluyveromyces marxianus FII 510700 grown on a lactose-based medium as a source of a natural bioemulsifier. Journal of Industrial Microbiology & Biotechnology, 30(12), 715-20.
Lukondeh T, Ashbolt NJ, Rogers PL. Evaluation of Kluyveromyces Marxianus FII 510700 Grown On a Lactose-based Medium as a Source of a Natural Bioemulsifier. J Ind Microbiol Biotechnol. 2003;30(12):715-20. PubMed PMID: 14689315.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of Kluyveromyces marxianus FII 510700 grown on a lactose-based medium as a source of a natural bioemulsifier. AU - Lukondeh,Tredwell, AU - Ashbolt,Nicholas J, AU - Rogers,Peter L, Y1 - 2003/12/19/ PY - 2003/04/14/received PY - 2003/11/07/accepted PY - 2003/12/23/pubmed PY - 2004/4/7/medline PY - 2003/12/23/entrez SP - 715 EP - 20 JF - Journal of industrial microbiology & biotechnology JO - J Ind Microbiol Biotechnol VL - 30 IS - 12 N2 - Mannoprotein with emulsification properties was extracted from the cell walls of Kluyveromyces marxianus grown on a lactose-based medium by autoclaving cells in a citrate buffer at pH 7. The purified product was evaluated for chemical and physical stability to establish its potential use as a natural emulsifier in processed foods. The yield of purified bioemulsifier from this strain of K. marxianus was 4-7% of the original dry cell weight. The purified product, at a concentration of 12 g l(-1), formed emulsions that were stable for 3 months when subjected to a range of pH (3-11) and NaCl concentrations (2-50 g l(-1)). The composition of this mannoprotein was 90% carbohydrate (mannan) and 4-6% protein. These values are similar to mannoprotein extracted from cells of Saccharomyces cerevisiae, which is the traditional source. Consequently K. marxianus cultivated on a low-cost lactose-based medium such as whey, a lactose-rich clean waste of the dairy industry, could be developed as a source of bioemulsifier for use in the food industry. SN - 1367-5435 UR - https://www.unboundmedicine.com/medline/citation/14689315/Evaluation_of_Kluyveromyces_marxianus_FII_510700_grown_on_a_lactose_based_medium_as_a_source_of_a_natural_bioemulsifier_ DB - PRIME DP - Unbound Medicine ER -