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Proanthocyanidin composition in the seed coat of lentils (Lens culinaris L.).
J Agric Food Chem. 2003 Dec 31; 51(27):7999-8004.JA

Abstract

Lentils (Lens culinaris L.) are a popular food in many countries. However, little is known about their phenolic composition. Because polyphenols in lentils are located essentially in their seed coat, the objective of this work was to study the composition of proanthocyanidins, the major group of polyphenols, in this part of the tissue. The use of C(18) Sep-Pak cartridges permitted the fractionation of lentil seed coat extract into monomer, oligomer, and polymer proanthocyanidin fractions. Subsequent thiolysis of oligomer and polymer fractions followed by HPLC analysis allowed the mean degree of polymerization (mDP) and the structural composition of proanthocyanidins to be determined. A fractionation of lentil seed coat extracts on a polyamide column followed by HPLC and HPLC-DAD-MS analyses was used to identify the individual proanthocyanidins. The results showed that the major monomeric flavan-3-ol was (+) catechin-3-glucose, with lesser amounts of (+)-catechin and (-)-epicatechin. In the oligomer fraction, various dimer, trimer, and tetramer proanthocyanidins constituted of catechin, gallocatechin, and catechin gallate units were identified, and several procyanidins and prodelphinidins from pentamers to nonamers constitute the polymer fraction. The most abundant proanthocyanidins in the seed coat of lentils are the polymers (65-75%), with a mDP of 7-9, followed by the oligomers (20-30%), with a mDP of 4-5.

Authors+Show Affiliations

Instituto de Fermentaciones Industriales, Juan de la Cierva 3, 28006 Madrid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14690386

Citation

Dueñas, M, et al. "Proanthocyanidin Composition in the Seed Coat of Lentils (Lens Culinaris L.)." Journal of Agricultural and Food Chemistry, vol. 51, no. 27, 2003, pp. 7999-8004.
Dueñas M, Sun B, Hernández T, et al. Proanthocyanidin composition in the seed coat of lentils (Lens culinaris L.). J Agric Food Chem. 2003;51(27):7999-8004.
Dueñas, M., Sun, B., Hernández, T., Estrella, I., & Spranger, M. I. (2003). Proanthocyanidin composition in the seed coat of lentils (Lens culinaris L.). Journal of Agricultural and Food Chemistry, 51(27), 7999-8004.
Dueñas M, et al. Proanthocyanidin Composition in the Seed Coat of Lentils (Lens Culinaris L.). J Agric Food Chem. 2003 Dec 31;51(27):7999-8004. PubMed PMID: 14690386.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proanthocyanidin composition in the seed coat of lentils (Lens culinaris L.). AU - Dueñas,M, AU - Sun,Baoshan, AU - Hernández,Teresa, AU - Estrella,Isabel, AU - Spranger,M Isabel, PY - 2003/12/24/pubmed PY - 2004/3/6/medline PY - 2003/12/24/entrez SP - 7999 EP - 8004 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 51 IS - 27 N2 - Lentils (Lens culinaris L.) are a popular food in many countries. However, little is known about their phenolic composition. Because polyphenols in lentils are located essentially in their seed coat, the objective of this work was to study the composition of proanthocyanidins, the major group of polyphenols, in this part of the tissue. The use of C(18) Sep-Pak cartridges permitted the fractionation of lentil seed coat extract into monomer, oligomer, and polymer proanthocyanidin fractions. Subsequent thiolysis of oligomer and polymer fractions followed by HPLC analysis allowed the mean degree of polymerization (mDP) and the structural composition of proanthocyanidins to be determined. A fractionation of lentil seed coat extracts on a polyamide column followed by HPLC and HPLC-DAD-MS analyses was used to identify the individual proanthocyanidins. The results showed that the major monomeric flavan-3-ol was (+) catechin-3-glucose, with lesser amounts of (+)-catechin and (-)-epicatechin. In the oligomer fraction, various dimer, trimer, and tetramer proanthocyanidins constituted of catechin, gallocatechin, and catechin gallate units were identified, and several procyanidins and prodelphinidins from pentamers to nonamers constitute the polymer fraction. The most abundant proanthocyanidins in the seed coat of lentils are the polymers (65-75%), with a mDP of 7-9, followed by the oligomers (20-30%), with a mDP of 4-5. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/14690386/Proanthocyanidin_composition_in_the_seed_coat_of_lentils__Lens_culinaris_L___ L2 - https://doi.org/10.1021/jf0303215 DB - PRIME DP - Unbound Medicine ER -