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Gamma-irradiation enhances apoptosis induced by cannabidiol, a non-psychotropic cannabinoid, in cultured HL-60 myeloblastic leukemia cells.
Leuk Lymphoma. 2003 Oct; 44(10):1767-73.LL

Abstract

Two non-psychotropic cannabinoids, cannabidiol (CBD) and cannabidiol-dimethylheptyl (CBD-DMH), induced apoptosis in a human acute myeloid leukemia (AML) HL-60 cell line. Apoptosis was determined by staining with bisBenzimide and propidium iodide. A dose dependent increase of apoptosis was noted, reaching 61 and 43% with 8 microg/ml CBD and 15 microg/ml CBD-DMH, respectively, after a 24 h treatment. Prior exposure of the cells to gamma-irradiation (800 cGy) markedly enhanced apoptosis, reaching values of 93 and 95%, respectively. Human monocytes from normal individuals were resistant to either cannabinoids or gamma-irradiation. Caspase-3 activation was observed after the cannabinoid treatment, and may represent a mechanism for the apoptosis. Our data suggest a possible new approach to treatment of AML.

Authors+Show Affiliations

Gallily R
The Lautenberg Center for General and Tumor Immunology, The Hebrew University Medical Faculty, Ein Kerem Campus, Jerusalem 1120, Israel. rgallily@md.huji.ac.il
Even-Chena T
No affiliation info available
Katzavian G
No affiliation info available
Lehmann D
No affiliation info available
Dagan A
No affiliation info available
Mechoulam R
No affiliation info available

MeSH

ApoptosisCannabidiolCaspase 3CaspasesEnzyme ActivationGamma RaysHL-60 CellsHumansMonocytes

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14692532

Citation

Gallily, Ruth, et al. "Gamma-irradiation Enhances Apoptosis Induced By Cannabidiol, a Non-psychotropic Cannabinoid, in Cultured HL-60 Myeloblastic Leukemia Cells." Leukemia & Lymphoma, vol. 44, no. 10, 2003, pp. 1767-73.
Gallily R, Even-Chena T, Katzavian G, et al. Gamma-irradiation enhances apoptosis induced by cannabidiol, a non-psychotropic cannabinoid, in cultured HL-60 myeloblastic leukemia cells. Leuk Lymphoma. 2003;44(10):1767-73.
Gallily, R., Even-Chena, T., Katzavian, G., Lehmann, D., Dagan, A., & Mechoulam, R. (2003). Gamma-irradiation enhances apoptosis induced by cannabidiol, a non-psychotropic cannabinoid, in cultured HL-60 myeloblastic leukemia cells. Leukemia & Lymphoma, 44(10), 1767-73.
Gallily R, et al. Gamma-irradiation Enhances Apoptosis Induced By Cannabidiol, a Non-psychotropic Cannabinoid, in Cultured HL-60 Myeloblastic Leukemia Cells. Leuk Lymphoma. 2003;44(10):1767-73. PubMed PMID: 14692532.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gamma-irradiation enhances apoptosis induced by cannabidiol, a non-psychotropic cannabinoid, in cultured HL-60 myeloblastic leukemia cells. AU - Gallily,Ruth, AU - Even-Chena,Tal, AU - Katzavian,Galia, AU - Lehmann,Dan, AU - Dagan,Arie, AU - Mechoulam,Raphael, PY - 2003/12/25/pubmed PY - 2004/6/23/medline PY - 2003/12/25/entrez SP - 1767 EP - 73 JF - Leukemia & lymphoma JO - Leuk Lymphoma VL - 44 IS - 10 N2 - Two non-psychotropic cannabinoids, cannabidiol (CBD) and cannabidiol-dimethylheptyl (CBD-DMH), induced apoptosis in a human acute myeloid leukemia (AML) HL-60 cell line. Apoptosis was determined by staining with bisBenzimide and propidium iodide. A dose dependent increase of apoptosis was noted, reaching 61 and 43% with 8 microg/ml CBD and 15 microg/ml CBD-DMH, respectively, after a 24 h treatment. Prior exposure of the cells to gamma-irradiation (800 cGy) markedly enhanced apoptosis, reaching values of 93 and 95%, respectively. Human monocytes from normal individuals were resistant to either cannabinoids or gamma-irradiation. Caspase-3 activation was observed after the cannabinoid treatment, and may represent a mechanism for the apoptosis. Our data suggest a possible new approach to treatment of AML. SN - 1042-8194 UR - https://www.unboundmedicine.com/medline/citation/14692532/Gamma_irradiation_enhances_apoptosis_induced_by_cannabidiol_a_non_psychotropic_cannabinoid_in_cultured_HL_60_myeloblastic_leukemia_cells_ DB - PRIME DP - Unbound Medicine ER -