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Dietary regulation of fat oxidative gene expression in different skeletal muscle fiber types.
Obes Res. 2003 Dec; 11(12):1471-9.OR

Abstract

OBJECTIVE

To determine the effect of a high-fat diet on the expression of genes important for fat oxidation, the protein abundance of the transcription factors peroxisome proliferator-activated receptor (PPAR) isoforms alpha and gamma, and selected enzyme activities in type I and II skeletal muscle.

RESEARCH METHODS AND PROCEDURES

Sprague-Dawley rats consumed either a high-fat (HF: 78% energy, n = 8) or high-carbohydrate (64% energy, n = 8) diet for 8 weeks while remaining sedentary.

RESULTS

The expression of genes important for fat oxidation tended to increase in both type I (soleus) and type II (extensor digitorum longus) fiber types after an HF dietary intervention. However, the expression of muscle type carnitine palmitoyltransferase I was not increased in extensor digitorum longus. Analysis of the gene expression of both peroxisome proliferator-activated receptor-gamma coactivator and fork-head transcription factor O1 demonstrated no alteration in response to the HF diet. Similarly, PPARalpha and PPARgamma protein levels were also not altered by the HF diet.

DISCUSSION

An HF diet increased the expression of an array of genes involved in lipid metabolism, with only subtle differences evident in the response within differing skeletal muscle fiber types. Despite changes in gene expression, there were no effects of diet on peroxisome proliferator-activated receptor-gamma coactivator and fork-head transcription factor O1 mRNA and the protein abundance of PPARalpha and PPARgamma.

Authors+Show Affiliations

School of Health Sciences, Deakin University, Burwood, Victoria, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14694211

Citation

McAinch, Andrew J., et al. "Dietary Regulation of Fat Oxidative Gene Expression in Different Skeletal Muscle Fiber Types." Obesity Research, vol. 11, no. 12, 2003, pp. 1471-9.
McAinch AJ, Lee JS, Bruce CR, et al. Dietary regulation of fat oxidative gene expression in different skeletal muscle fiber types. Obes Res. 2003;11(12):1471-9.
McAinch, A. J., Lee, J. S., Bruce, C. R., Tunstall, R. J., Hawley, J. A., & Cameron-Smith, D. (2003). Dietary regulation of fat oxidative gene expression in different skeletal muscle fiber types. Obesity Research, 11(12), 1471-9.
McAinch AJ, et al. Dietary Regulation of Fat Oxidative Gene Expression in Different Skeletal Muscle Fiber Types. Obes Res. 2003;11(12):1471-9. PubMed PMID: 14694211.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary regulation of fat oxidative gene expression in different skeletal muscle fiber types. AU - McAinch,Andrew J, AU - Lee,Jong-Sam, AU - Bruce,Clinton R, AU - Tunstall,Rebecca J, AU - Hawley,John A, AU - Cameron-Smith,David, PY - 2003/12/25/pubmed PY - 2004/4/17/medline PY - 2003/12/25/entrez SP - 1471 EP - 9 JF - Obesity research JO - Obes. Res. VL - 11 IS - 12 N2 - OBJECTIVE: To determine the effect of a high-fat diet on the expression of genes important for fat oxidation, the protein abundance of the transcription factors peroxisome proliferator-activated receptor (PPAR) isoforms alpha and gamma, and selected enzyme activities in type I and II skeletal muscle. RESEARCH METHODS AND PROCEDURES: Sprague-Dawley rats consumed either a high-fat (HF: 78% energy, n = 8) or high-carbohydrate (64% energy, n = 8) diet for 8 weeks while remaining sedentary. RESULTS: The expression of genes important for fat oxidation tended to increase in both type I (soleus) and type II (extensor digitorum longus) fiber types after an HF dietary intervention. However, the expression of muscle type carnitine palmitoyltransferase I was not increased in extensor digitorum longus. Analysis of the gene expression of both peroxisome proliferator-activated receptor-gamma coactivator and fork-head transcription factor O1 demonstrated no alteration in response to the HF diet. Similarly, PPARalpha and PPARgamma protein levels were also not altered by the HF diet. DISCUSSION: An HF diet increased the expression of an array of genes involved in lipid metabolism, with only subtle differences evident in the response within differing skeletal muscle fiber types. Despite changes in gene expression, there were no effects of diet on peroxisome proliferator-activated receptor-gamma coactivator and fork-head transcription factor O1 mRNA and the protein abundance of PPARalpha and PPARgamma. SN - 1071-7323 UR - https://www.unboundmedicine.com/medline/citation/14694211/Dietary_regulation_of_fat_oxidative_gene_expression_in_different_skeletal_muscle_fiber_types_ L2 - https://doi.org/10.1038/oby.2003.197 DB - PRIME DP - Unbound Medicine ER -