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Effect of NF-kappaB, survivin, Bcl-2 and Caspase3 on apoptosis of gastric cancer cells induced by tumor necrosis factor related apoptosis inducing ligand.
World J Gastroenterol. 2004 Jan; 10(1):22-5.WJ

Abstract

AIM

To study the effect of NF-kappaB, survivin, Bcl-2 and Caspase3 on tumor necrosis factors related apoptosis inducing ligand (TRAIL) induced apoptosis of gastric cancer cells.

METHODS

Gastric cancer cells of SGC-7901, MKN28, MKN45 and AGS lines were cultured in PRMI-1640 medium and the apoptosis rates of the cells of 4 lines were observed after treatment of tumor necrosis factors related apoptosis inducing ligand (TRAIL) with a flow cytometer. The expression of NF-kappaB, survivin, Bcl-2 and Caspase3 in gastric cancer cells of 4 lines was analyzed with Western blot.

RESULTS

After the gastric cancer cells were exposed to TRAIL 300 ng/ml for 24 hours, the apoptosis rate was 36.05%, 20.27%, 16.50% and 11.80% in MKN28, MKN45, AGS and SGC-7901cells respectively. Western blot revealed that the expressions of NF-kappaB and survivin were lower in MKN28 cells than in MKN45, AGS and SGC-7901 cells. In contrast, the expression of Caspase3 was higher in MKN28 cells than in MKN45, AGS and SGC-7901 cells.

CONCLUSION

There is a selectivity of TRAIL potency to induce apoptosis in gastric cancer cells of different cell lines. The anticancer potency of TRAIL is associated with the decreased expression of NF-kappaB and survivin and increased expression of Caspase3 of gastric cancer cells.

Authors+Show Affiliations

Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14695762

Citation

Yang, Liu-Qin, et al. "Effect of NF-kappaB, Survivin, Bcl-2 and Caspase3 On Apoptosis of Gastric Cancer Cells Induced By Tumor Necrosis Factor Related Apoptosis Inducing Ligand." World Journal of Gastroenterology, vol. 10, no. 1, 2004, pp. 22-5.
Yang LQ, Fang DC, Wang RQ, et al. Effect of NF-kappaB, survivin, Bcl-2 and Caspase3 on apoptosis of gastric cancer cells induced by tumor necrosis factor related apoptosis inducing ligand. World J Gastroenterol. 2004;10(1):22-5.
Yang, L. Q., Fang, D. C., Wang, R. Q., & Yang, S. M. (2004). Effect of NF-kappaB, survivin, Bcl-2 and Caspase3 on apoptosis of gastric cancer cells induced by tumor necrosis factor related apoptosis inducing ligand. World Journal of Gastroenterology, 10(1), 22-5.
Yang LQ, et al. Effect of NF-kappaB, Survivin, Bcl-2 and Caspase3 On Apoptosis of Gastric Cancer Cells Induced By Tumor Necrosis Factor Related Apoptosis Inducing Ligand. World J Gastroenterol. 2004;10(1):22-5. PubMed PMID: 14695762.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of NF-kappaB, survivin, Bcl-2 and Caspase3 on apoptosis of gastric cancer cells induced by tumor necrosis factor related apoptosis inducing ligand. AU - Yang,Liu-Qin, AU - Fang,Dian-Chun, AU - Wang,Rong-Quan, AU - Yang,Shi-Ming, PY - 2003/12/27/pubmed PY - 2004/2/12/medline PY - 2003/12/27/entrez SP - 22 EP - 5 JF - World journal of gastroenterology JO - World J Gastroenterol VL - 10 IS - 1 N2 - AIM: To study the effect of NF-kappaB, survivin, Bcl-2 and Caspase3 on tumor necrosis factors related apoptosis inducing ligand (TRAIL) induced apoptosis of gastric cancer cells. METHODS: Gastric cancer cells of SGC-7901, MKN28, MKN45 and AGS lines were cultured in PRMI-1640 medium and the apoptosis rates of the cells of 4 lines were observed after treatment of tumor necrosis factors related apoptosis inducing ligand (TRAIL) with a flow cytometer. The expression of NF-kappaB, survivin, Bcl-2 and Caspase3 in gastric cancer cells of 4 lines was analyzed with Western blot. RESULTS: After the gastric cancer cells were exposed to TRAIL 300 ng/ml for 24 hours, the apoptosis rate was 36.05%, 20.27%, 16.50% and 11.80% in MKN28, MKN45, AGS and SGC-7901cells respectively. Western blot revealed that the expressions of NF-kappaB and survivin were lower in MKN28 cells than in MKN45, AGS and SGC-7901 cells. In contrast, the expression of Caspase3 was higher in MKN28 cells than in MKN45, AGS and SGC-7901 cells. CONCLUSION: There is a selectivity of TRAIL potency to induce apoptosis in gastric cancer cells of different cell lines. The anticancer potency of TRAIL is associated with the decreased expression of NF-kappaB and survivin and increased expression of Caspase3 of gastric cancer cells. SN - 1007-9327 UR - https://www.unboundmedicine.com/medline/citation/14695762/Effect_of_NF_kappaB_survivin_Bcl_2_and_Caspase3_on_apoptosis_of_gastric_cancer_cells_induced_by_tumor_necrosis_factor_related_apoptosis_inducing_ligand_ L2 - https://www.wjgnet.com/1007-9327/full/v10/i1/22.htm DB - PRIME DP - Unbound Medicine ER -