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Effects of water temperature and diets containing palm oil on fatty acid desaturation and oxidation in hepatocytes and intestinal enterocytes of rainbow trout (Oncorhynchus mykiss).

Abstract

Food grade fisheries have reached their sustainable limits while aquaculture production has increased to meet consumer demands. However, for growth in aquaculture to continue and utilise sustainable, feeding ingredients, alternatives to fish oil (FO), the predominant lipid component of fish diets, must be developed. Therefore, there is currently considerable interest in the regulation of fatty acid metabolism in fish in order to determine strategies for the best use of plant oils in diets for commercially important cultured fish species. Plant oils are characteristically rich in C18 polyunsaturated fatty acids (PUFA) but devoid of C20 and C22 highly unsaturated fatty acids (HUFA) found in FO. The fatty acyl desaturase enzyme activities involved in the biosynthesis of HUFA from PUFA are known to be under nutritional regulation and can be increased in fish fed diets rich in plant oils. However, fatty acid desaturase activity is also known to be modulated by water temperature in fish. The present study aimed to investigate the interaction between water temperature and diet in the regulation of fatty acid metabolism in rainbow trout. Trout, acclimatized to 7, 11 or 15 degrees C, were fed for 4 weeks on diets in which the FO was replaced in a graded manner by palm oil. At the end of the trial, fatty acyl desaturation/elongation and beta-oxidation activities were determined in isolated hepatocytes and intestinal enterocytes using [1-14C]18:3n-3 as substrate, and samples of liver were collected for analysis of lipid and fatty acid composition. The most obvious effect of temperature was that fatty acid desaturation/elongation and beta-oxidation were reduced in both hepatocytes and intestinal enterocytes from fish maintained at the highest water temperature (15 degrees C). There were differences between the two tissues with the highest desaturation/elongation and beta-oxidation activities tending to be in fish held at 11 degrees C in the case of hepatocytes, but 7 degrees C in enterocytes. Correlations between fatty acid metabolism and dietary palm oil were most clearly observed in desaturation/elongation activities in both hepatocytes and enterocytes at 11 degrees C. The highest beta-oxidation activities were generally observed in fish fed FO alone in both hepatocytes and enterocytes with palm oil having differential effects in the two cell types.

Authors+Show Affiliations

Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, Scotland, UK. d.r.tocher@stir.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14698910

Citation

Tocher, Douglas R., et al. "Effects of Water Temperature and Diets Containing Palm Oil On Fatty Acid Desaturation and Oxidation in Hepatocytes and Intestinal Enterocytes of Rainbow Trout (Oncorhynchus Mykiss)." Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, vol. 137, no. 1, 2004, pp. 49-63.
Tocher DR, Fonseca-Madrigal J, Dick JR, et al. Effects of water temperature and diets containing palm oil on fatty acid desaturation and oxidation in hepatocytes and intestinal enterocytes of rainbow trout (Oncorhynchus mykiss). Comp Biochem Physiol B, Biochem Mol Biol. 2004;137(1):49-63.
Tocher, D. R., Fonseca-Madrigal, J., Dick, J. R., Ng, W. K., Bell, J. G., & Campbell, P. J. (2004). Effects of water temperature and diets containing palm oil on fatty acid desaturation and oxidation in hepatocytes and intestinal enterocytes of rainbow trout (Oncorhynchus mykiss). Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 137(1), pp. 49-63.
Tocher DR, et al. Effects of Water Temperature and Diets Containing Palm Oil On Fatty Acid Desaturation and Oxidation in Hepatocytes and Intestinal Enterocytes of Rainbow Trout (Oncorhynchus Mykiss). Comp Biochem Physiol B, Biochem Mol Biol. 2004;137(1):49-63. PubMed PMID: 14698910.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of water temperature and diets containing palm oil on fatty acid desaturation and oxidation in hepatocytes and intestinal enterocytes of rainbow trout (Oncorhynchus mykiss). AU - Tocher,Douglas R, AU - Fonseca-Madrigal,Jorge, AU - Dick,James R, AU - Ng,Wing-Keong, AU - Bell,J Gordon, AU - Campbell,Patrick J, PY - 2003/12/31/pubmed PY - 2004/8/18/medline PY - 2003/12/31/entrez SP - 49 EP - 63 JF - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology JO - Comp. Biochem. Physiol. B, Biochem. Mol. Biol. VL - 137 IS - 1 N2 - Food grade fisheries have reached their sustainable limits while aquaculture production has increased to meet consumer demands. However, for growth in aquaculture to continue and utilise sustainable, feeding ingredients, alternatives to fish oil (FO), the predominant lipid component of fish diets, must be developed. Therefore, there is currently considerable interest in the regulation of fatty acid metabolism in fish in order to determine strategies for the best use of plant oils in diets for commercially important cultured fish species. Plant oils are characteristically rich in C18 polyunsaturated fatty acids (PUFA) but devoid of C20 and C22 highly unsaturated fatty acids (HUFA) found in FO. The fatty acyl desaturase enzyme activities involved in the biosynthesis of HUFA from PUFA are known to be under nutritional regulation and can be increased in fish fed diets rich in plant oils. However, fatty acid desaturase activity is also known to be modulated by water temperature in fish. The present study aimed to investigate the interaction between water temperature and diet in the regulation of fatty acid metabolism in rainbow trout. Trout, acclimatized to 7, 11 or 15 degrees C, were fed for 4 weeks on diets in which the FO was replaced in a graded manner by palm oil. At the end of the trial, fatty acyl desaturation/elongation and beta-oxidation activities were determined in isolated hepatocytes and intestinal enterocytes using [1-14C]18:3n-3 as substrate, and samples of liver were collected for analysis of lipid and fatty acid composition. The most obvious effect of temperature was that fatty acid desaturation/elongation and beta-oxidation were reduced in both hepatocytes and intestinal enterocytes from fish maintained at the highest water temperature (15 degrees C). There were differences between the two tissues with the highest desaturation/elongation and beta-oxidation activities tending to be in fish held at 11 degrees C in the case of hepatocytes, but 7 degrees C in enterocytes. Correlations between fatty acid metabolism and dietary palm oil were most clearly observed in desaturation/elongation activities in both hepatocytes and enterocytes at 11 degrees C. The highest beta-oxidation activities were generally observed in fish fed FO alone in both hepatocytes and enterocytes with palm oil having differential effects in the two cell types. SN - 1096-4959 UR - https://www.unboundmedicine.com/medline/citation/14698910/Effects_of_water_temperature_and_diets_containing_palm_oil_on_fatty_acid_desaturation_and_oxidation_in_hepatocytes_and_intestinal_enterocytes_of_rainbow_trout__Oncorhynchus_mykiss__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1096495903003130 DB - PRIME DP - Unbound Medicine ER -