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Over-expression of a barley aquaporin increased the shoot/root ratio and raised salt sensitivity in transgenic rice plants.
Plant Cell Physiol. 2003 Dec; 44(12):1378-83.PC

Abstract

Barley HvPIP2;1 is a plasma membrane aquaporin and its expression was down-regulated after salt stress in barley [Katsuhara et al. (2002) Plant Cell Physiol. 43: 885]. We produced and analyzed transgenic rice plants over-expressing barley HvPIP2;1 in the present study. Over-expression of HvPIP2;1 increased (1) radial hydraulic conductivity of roots (Lp(r)) to 140%, and (2) the mass ratio of shoot to root up to 150%. In these transgenic rice plants under salt stress of 100 mM NaCl, growth reduction was greater than in non-transgenic plants. A decrease in shoot water content (from 79% to 61%) and reduction of root mass or shoot mass (both less than 40% of non-stressed plants) were observed in transgenic plants under salt stress for 2 weeks. These results indicated that over-expression of HvPIP2;1 makes rice plants sensitive to 100 mM NaCl. The possible involvement of aquaporins in salt tolerance is discussed.

Authors+Show Affiliations

Katsuhara M
Research Institute for Bioresources, Okayama University, Kurashiki, 710-0046 Japan. kmaki@rib.okayama-u.ac.jp
Koshio K
No affiliation info available
Shibasaka M
No affiliation info available
Hayashi Y
No affiliation info available
Hayakawa T
No affiliation info available
Kasamo K
No affiliation info available

MeSH

Adaptation, PhysiologicalAquaporinsGene Expression Regulation, PlantHordeumMolecular Sequence DataOryzaOsmotic PressurePlant ProteinsPlant RootsPlant ShootsPlants, Genetically ModifiedSodium Chloride

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14701933

Citation

Katsuhara, Maki, et al. "Over-expression of a Barley Aquaporin Increased the Shoot/root Ratio and Raised Salt Sensitivity in Transgenic Rice Plants." Plant & Cell Physiology, vol. 44, no. 12, 2003, pp. 1378-83.
Katsuhara M, Koshio K, Shibasaka M, et al. Over-expression of a barley aquaporin increased the shoot/root ratio and raised salt sensitivity in transgenic rice plants. Plant Cell Physiol. 2003;44(12):1378-83.
Katsuhara, M., Koshio, K., Shibasaka, M., Hayashi, Y., Hayakawa, T., & Kasamo, K. (2003). Over-expression of a barley aquaporin increased the shoot/root ratio and raised salt sensitivity in transgenic rice plants. Plant & Cell Physiology, 44(12), 1378-83.
Katsuhara M, et al. Over-expression of a Barley Aquaporin Increased the Shoot/root Ratio and Raised Salt Sensitivity in Transgenic Rice Plants. Plant Cell Physiol. 2003;44(12):1378-83. PubMed PMID: 14701933.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Over-expression of a barley aquaporin increased the shoot/root ratio and raised salt sensitivity in transgenic rice plants. AU - Katsuhara,Maki, AU - Koshio,Kazuki, AU - Shibasaka,Mineo, AU - Hayashi,Yasuyuki, AU - Hayakawa,Takahiko, AU - Kasamo,Kunihiro, PY - 2004/1/1/pubmed PY - 2004/3/27/medline PY - 2004/1/1/entrez SP - 1378 EP - 83 JF - Plant & cell physiology JO - Plant Cell Physiol VL - 44 IS - 12 N2 - Barley HvPIP2;1 is a plasma membrane aquaporin and its expression was down-regulated after salt stress in barley [Katsuhara et al. (2002) Plant Cell Physiol. 43: 885]. We produced and analyzed transgenic rice plants over-expressing barley HvPIP2;1 in the present study. Over-expression of HvPIP2;1 increased (1) radial hydraulic conductivity of roots (Lp(r)) to 140%, and (2) the mass ratio of shoot to root up to 150%. In these transgenic rice plants under salt stress of 100 mM NaCl, growth reduction was greater than in non-transgenic plants. A decrease in shoot water content (from 79% to 61%) and reduction of root mass or shoot mass (both less than 40% of non-stressed plants) were observed in transgenic plants under salt stress for 2 weeks. These results indicated that over-expression of HvPIP2;1 makes rice plants sensitive to 100 mM NaCl. The possible involvement of aquaporins in salt tolerance is discussed. SN - 0032-0781 UR - https://www.unboundmedicine.com/medline/citation/14701933/Over_expression_of_a_barley_aquaporin_increased_the_shoot/root_ratio_and_raised_salt_sensitivity_in_transgenic_rice_plants_ DB - PRIME DP - Unbound Medicine ER -