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[A study on the role of vascular endothelial growth factor in emphysema of rat caused by smog exposure].
Zhonghua Jie He He Hu Xi Za Zhi. 2003 Nov; 26(11):671-4.ZJ

Abstract

OBJECTIVE

To explore the role of vascular endothelial growth factor (VEGF) in emphysema of rat caused by smog exposure.

METHODS

Thirty-six 12-week male rats were randomly divided into 2 groups: a smog exposure group (S group), and a normal control group (N group). S group rats were randomly subdivided into 3 groups: S(1) group, S(2) group, S(3) group, which were exposed to smog for 2 weeks, 4 weeks, 8 weeks, respectively; N group rats were randomly subdivided into 3 groups: N(1) group, N(2) group, N(3) group, which were raised in normal oxygen condition for 0 week, 4 weeks, 8 weeks, respectively. The expressions of VEGF mRNA and VEGF protein and kinase-insert domain containing receptor (KDR) protein were determined by reverse transcription-polymerase chain reaction (RT-PCR) and modified SABC immunohistochemistry assay separately. The pathological change in smog exposure rat lung was determined by HE staining. MLI and MAN were determined as an index of emphysema. Variance analysis, nonparametric analysis and correlate analysis were conducted in SPSS 10.0.

RESULTS

(1) There were airway inflammation in S group rat lungs, and an early-emphysema-like change in S(3) group rat lungs: MAN in S(3) group was significantly decreased compared with N(3) group, MLI in S(3) group was significantly increased compared with N(3) group (P < 0.05). (2) VEGF189 mRNA in lung and VEGF protein in alveolar and bronchial epithelium of rats in S(1) group were significantly lower than those in N(1) group (P < 0.01); VEGF189 mRNA, VEGF165 mRNA and VEGF121 mRNA of lung tissue and VEGF protein in alveolar and bronchial epithelium of rats in S(2) group were significantly lower than those in N(2) group (P < 0.01), too; VEGF protein in alveolar and bronchial epithelium and KDR expression in pulmonary vascular endothelium of rats in S(3) group was lower than that in N(3) group (P < 0.01, P < 0.01, P < 0.05), but VEGF mRNA have no difference (P > 0.05). (3) VEGF protein expression in alveolar epithelium and bronchial epithelium had a positive correlation with MAN (r = 0.43, r = 0.37, P < 0.05) and an inverse correlation with MLI (r = -0.42, r = -0.37, P < 0.05).

CONCLUSIONS

Smog exposure decrease the expression of VEGF and KDR in rat lung. VEGF might involve in the pathology of emphysema caused by smog exposure.

Authors+Show Affiliations

Guangzhou Institute of Respiratory Diseases, First Affiliated Hospital, Guangzhou Medical College, Guangzhou 510120, China.No affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

14703440

Citation

Chen, Jin-long, and Pi-xin Ran. "[A Study On the Role of Vascular Endothelial Growth Factor in Emphysema of Rat Caused By Smog Exposure]." Zhonghua Jie He He Hu Xi Za Zhi = Zhonghua Jiehe He Huxi Zazhi = Chinese Journal of Tuberculosis and Respiratory Diseases, vol. 26, no. 11, 2003, pp. 671-4.
Chen JL, Ran PX. [A study on the role of vascular endothelial growth factor in emphysema of rat caused by smog exposure]. Zhonghua Jie He He Hu Xi Za Zhi. 2003;26(11):671-4.
Chen, J. L., & Ran, P. X. (2003). [A study on the role of vascular endothelial growth factor in emphysema of rat caused by smog exposure]. Zhonghua Jie He He Hu Xi Za Zhi = Zhonghua Jiehe He Huxi Zazhi = Chinese Journal of Tuberculosis and Respiratory Diseases, 26(11), 671-4.
Chen JL, Ran PX. [A Study On the Role of Vascular Endothelial Growth Factor in Emphysema of Rat Caused By Smog Exposure]. Zhonghua Jie He He Hu Xi Za Zhi. 2003;26(11):671-4. PubMed PMID: 14703440.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [A study on the role of vascular endothelial growth factor in emphysema of rat caused by smog exposure]. AU - Chen,Jin-long, AU - Ran,Pi-xin, PY - 2004/1/3/pubmed PY - 2004/5/28/medline PY - 2004/1/3/entrez SP - 671 EP - 4 JF - Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases JO - Zhonghua Jie He He Hu Xi Za Zhi VL - 26 IS - 11 N2 - OBJECTIVE: To explore the role of vascular endothelial growth factor (VEGF) in emphysema of rat caused by smog exposure. METHODS: Thirty-six 12-week male rats were randomly divided into 2 groups: a smog exposure group (S group), and a normal control group (N group). S group rats were randomly subdivided into 3 groups: S(1) group, S(2) group, S(3) group, which were exposed to smog for 2 weeks, 4 weeks, 8 weeks, respectively; N group rats were randomly subdivided into 3 groups: N(1) group, N(2) group, N(3) group, which were raised in normal oxygen condition for 0 week, 4 weeks, 8 weeks, respectively. The expressions of VEGF mRNA and VEGF protein and kinase-insert domain containing receptor (KDR) protein were determined by reverse transcription-polymerase chain reaction (RT-PCR) and modified SABC immunohistochemistry assay separately. The pathological change in smog exposure rat lung was determined by HE staining. MLI and MAN were determined as an index of emphysema. Variance analysis, nonparametric analysis and correlate analysis were conducted in SPSS 10.0. RESULTS: (1) There were airway inflammation in S group rat lungs, and an early-emphysema-like change in S(3) group rat lungs: MAN in S(3) group was significantly decreased compared with N(3) group, MLI in S(3) group was significantly increased compared with N(3) group (P < 0.05). (2) VEGF189 mRNA in lung and VEGF protein in alveolar and bronchial epithelium of rats in S(1) group were significantly lower than those in N(1) group (P < 0.01); VEGF189 mRNA, VEGF165 mRNA and VEGF121 mRNA of lung tissue and VEGF protein in alveolar and bronchial epithelium of rats in S(2) group were significantly lower than those in N(2) group (P < 0.01), too; VEGF protein in alveolar and bronchial epithelium and KDR expression in pulmonary vascular endothelium of rats in S(3) group was lower than that in N(3) group (P < 0.01, P < 0.01, P < 0.05), but VEGF mRNA have no difference (P > 0.05). (3) VEGF protein expression in alveolar epithelium and bronchial epithelium had a positive correlation with MAN (r = 0.43, r = 0.37, P < 0.05) and an inverse correlation with MLI (r = -0.42, r = -0.37, P < 0.05). CONCLUSIONS: Smog exposure decrease the expression of VEGF and KDR in rat lung. VEGF might involve in the pathology of emphysema caused by smog exposure. SN - 1001-0939 UR - https://www.unboundmedicine.com/medline/citation/14703440/[A_study_on_the_role_of_vascular_endothelial_growth_factor_in_emphysema_of_rat_caused_by_smog_exposure]_ DB - PRIME DP - Unbound Medicine ER -