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SC35 and heterogeneous nuclear ribonucleoprotein A/B proteins bind to a juxtaposed exonic splicing enhancer/exonic splicing silencer element to regulate HIV-1 tat exon 2 splicing.
J Biol Chem. 2004 Mar 12; 279(11):10077-84.JB

Abstract

Splicing of the human immunodeficiency virus, type 1, primary transcript is highly regulated. Maintaining the proper equilibrium among spliced, unspliced, and partially spliced isoforms is essential for the replication of the virus. Here we characterize a complex cis-acting element located in tat exon 2 that is required for the splicing regulation of the upstream intron. An exonic splicing enhancer (ESE) and an exonic splicing silencer (ESS) are both located within the regulatory element. Heterogeneous nuclear ribonucleoprotein (hnRNP) A/B proteins bind the ESS to repress splicing, whereas the SR protein SC35 binds the ESE to activate it. We show that the SC35 and the hnRNP A1 binding sites overlap within the juxtaposed ESE/ESS. We propose that hnRNP A1 binding to the ESS inhibits splicing of the upstream intron by directly masking the SC35 binding site.

Authors+Show Affiliations

Department of Molecular, Cellular and Developmental Biology and Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz 95064, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

14703516

Citation

Zahler, Alan M., et al. "SC35 and Heterogeneous Nuclear Ribonucleoprotein A/B Proteins Bind to a Juxtaposed Exonic Splicing Enhancer/exonic Splicing Silencer Element to Regulate HIV-1 Tat Exon 2 Splicing." The Journal of Biological Chemistry, vol. 279, no. 11, 2004, pp. 10077-84.
Zahler AM, Damgaard CK, Kjems J, et al. SC35 and heterogeneous nuclear ribonucleoprotein A/B proteins bind to a juxtaposed exonic splicing enhancer/exonic splicing silencer element to regulate HIV-1 tat exon 2 splicing. J Biol Chem. 2004;279(11):10077-84.
Zahler, A. M., Damgaard, C. K., Kjems, J., & Caputi, M. (2004). SC35 and heterogeneous nuclear ribonucleoprotein A/B proteins bind to a juxtaposed exonic splicing enhancer/exonic splicing silencer element to regulate HIV-1 tat exon 2 splicing. The Journal of Biological Chemistry, 279(11), 10077-84.
Zahler AM, et al. SC35 and Heterogeneous Nuclear Ribonucleoprotein A/B Proteins Bind to a Juxtaposed Exonic Splicing Enhancer/exonic Splicing Silencer Element to Regulate HIV-1 Tat Exon 2 Splicing. J Biol Chem. 2004 Mar 12;279(11):10077-84. PubMed PMID: 14703516.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SC35 and heterogeneous nuclear ribonucleoprotein A/B proteins bind to a juxtaposed exonic splicing enhancer/exonic splicing silencer element to regulate HIV-1 tat exon 2 splicing. AU - Zahler,Alan M, AU - Damgaard,Christian K, AU - Kjems,Jorgen, AU - Caputi,Massimo, Y1 - 2003/12/31/ PY - 2004/1/3/pubmed PY - 2004/5/20/medline PY - 2004/1/3/entrez SP - 10077 EP - 84 JF - The Journal of biological chemistry JO - J Biol Chem VL - 279 IS - 11 N2 - Splicing of the human immunodeficiency virus, type 1, primary transcript is highly regulated. Maintaining the proper equilibrium among spliced, unspliced, and partially spliced isoforms is essential for the replication of the virus. Here we characterize a complex cis-acting element located in tat exon 2 that is required for the splicing regulation of the upstream intron. An exonic splicing enhancer (ESE) and an exonic splicing silencer (ESS) are both located within the regulatory element. Heterogeneous nuclear ribonucleoprotein (hnRNP) A/B proteins bind the ESS to repress splicing, whereas the SR protein SC35 binds the ESE to activate it. We show that the SC35 and the hnRNP A1 binding sites overlap within the juxtaposed ESE/ESS. We propose that hnRNP A1 binding to the ESS inhibits splicing of the upstream intron by directly masking the SC35 binding site. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/14703516/SC35_and_heterogeneous_nuclear_ribonucleoprotein_A/B_proteins_bind_to_a_juxtaposed_exonic_splicing_enhancer/exonic_splicing_silencer_element_to_regulate_HIV_1_tat_exon_2_splicing_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9258(17)47658-7 DB - PRIME DP - Unbound Medicine ER -