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Quantitative analysis of herpes simplex virus genome in tears from patients with herpetic keratitis.
Cornea 2003; 22(7 Suppl):S55-60C

Abstract

PURPOSE

Herpetic keratitis is manifested in various corneal disorders, for example, dendritic keratitis, persistent epithelial defect, disciform keratitis, and endotheliitis. In this paper, we report on the quantity of herpes simplex virus (HSV) genome in tears from patients with various types of herpetic keratitis in an attempt to understand the role of HSV in these conditions.

METHODS

We collected tear samples from both eyes of 56 consecutive patients with herpetic keratitis who visited Kinki University Hospital between June 2000 and May 2002. All patients had unilateral herpetic keratitis: epithelial keratitis in 27 eyes; persistent epithelial defect in 6; active disciform stromal keratitis in 14; silent stromal keratitis in 6; and endotheliitis in 3. We measured levels of HSV genome in these tear samples using a real-time polymerase chain reaction (PCR) method.

RESULTS

In epithelial keratitis, HSV DNA was detected in all 27 samples from affected eyes (6.4 +/- 4.4 x 10(5) copies/sample). In persistent epithelial defect, HSV DNA was detected in all 6 samples from affected eyes (8.5 +/- 3.3 x 10(4) copies/sample). In active disciform stromal keratitis, HSV DNA was detected in 8 of the 14 affected eyes (1.4 +/- 1.1 x 10(5) copies/sample including zero values in negative samples). HSV DNA was not detected in samples from unaffected eyes or eyes affected by silent stromal keratitis or endotheliitis.

CONCLUSION

Real-time PCR is a useful method for quantifying HSV DNA in tear samples from patients with herpetic keratitis. Using this method, we demonstrate that HSV reproduction occurs in persistent epithelial defect and disciform stromal keratitis.

Authors+Show Affiliations

Department of Ophthalmology, Kinki University School of Medicine, Osaka-Sayama City, Osaka, Japan. fukuda@ganka.med.kindai.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14703708

Citation

Fukuda, Masahiko, et al. "Quantitative Analysis of Herpes Simplex Virus Genome in Tears From Patients With Herpetic Keratitis." Cornea, vol. 22, no. 7 Suppl, 2003, pp. S55-60.
Fukuda M, Deai T, Hibino T, et al. Quantitative analysis of herpes simplex virus genome in tears from patients with herpetic keratitis. Cornea. 2003;22(7 Suppl):S55-60.
Fukuda, M., Deai, T., Hibino, T., Higaki, S., Hayashi, K., & Shimomura, Y. (2003). Quantitative analysis of herpes simplex virus genome in tears from patients with herpetic keratitis. Cornea, 22(7 Suppl), pp. S55-60.
Fukuda M, et al. Quantitative Analysis of Herpes Simplex Virus Genome in Tears From Patients With Herpetic Keratitis. Cornea. 2003;22(7 Suppl):S55-60. PubMed PMID: 14703708.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative analysis of herpes simplex virus genome in tears from patients with herpetic keratitis. AU - Fukuda,Masahiko, AU - Deai,Tatsunori, AU - Hibino,Tsuyoshi, AU - Higaki,Shiro, AU - Hayashi,Kozaburo, AU - Shimomura,Yoshikazu, PY - 2004/1/6/pubmed PY - 2004/2/12/medline PY - 2004/1/6/entrez SP - S55 EP - 60 JF - Cornea JO - Cornea VL - 22 IS - 7 Suppl N2 - PURPOSE: Herpetic keratitis is manifested in various corneal disorders, for example, dendritic keratitis, persistent epithelial defect, disciform keratitis, and endotheliitis. In this paper, we report on the quantity of herpes simplex virus (HSV) genome in tears from patients with various types of herpetic keratitis in an attempt to understand the role of HSV in these conditions. METHODS: We collected tear samples from both eyes of 56 consecutive patients with herpetic keratitis who visited Kinki University Hospital between June 2000 and May 2002. All patients had unilateral herpetic keratitis: epithelial keratitis in 27 eyes; persistent epithelial defect in 6; active disciform stromal keratitis in 14; silent stromal keratitis in 6; and endotheliitis in 3. We measured levels of HSV genome in these tear samples using a real-time polymerase chain reaction (PCR) method. RESULTS: In epithelial keratitis, HSV DNA was detected in all 27 samples from affected eyes (6.4 +/- 4.4 x 10(5) copies/sample). In persistent epithelial defect, HSV DNA was detected in all 6 samples from affected eyes (8.5 +/- 3.3 x 10(4) copies/sample). In active disciform stromal keratitis, HSV DNA was detected in 8 of the 14 affected eyes (1.4 +/- 1.1 x 10(5) copies/sample including zero values in negative samples). HSV DNA was not detected in samples from unaffected eyes or eyes affected by silent stromal keratitis or endotheliitis. CONCLUSION: Real-time PCR is a useful method for quantifying HSV DNA in tear samples from patients with herpetic keratitis. Using this method, we demonstrate that HSV reproduction occurs in persistent epithelial defect and disciform stromal keratitis. SN - 0277-3740 UR - https://www.unboundmedicine.com/medline/citation/14703708/Quantitative_analysis_of_herpes_simplex_virus_genome_in_tears_from_patients_with_herpetic_keratitis_ L2 - http://Insights.ovid.com/pubmed?pmid=14703708 DB - PRIME DP - Unbound Medicine ER -