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Sensitization of U937 cells to heat shock by oxalomalate, a competitive inhibitor of NADP+-dependent isocitrate dehydrogenase.
Free Radic Res. 2003 Oct; 37(10):1099-105.FR

Abstract

Heat shock may increase oxidative stress due to increased production of reactive oxygen species (ROS) and/or the promotion of cellular oxidation events. Cytosolic NADP+-dependent isocitrate dehydrogenase (ICDH) in U937 cells produces NADPH, an essential reducing equivalent for the antioxidant system. The protective role of ICDH against heat shock in U937 cells was investigated in control and cells treated with oxlalomalate, a competitive inhibitor of ICDH. Upon exposure to heat shock, the viability was lower and the protein oxidation, lipid peroxidation and oxidative DNA damage were higher in oxalomalate-treated cells as compared to control cells. We also observed the significant increase in the endogenous production of ROS, as measured by the oxidation of 2'7'-dichlorodihydrofluorescin in U937 cells treated with oxalomalate. These results suggest that ICDH plays an important role as an antioxidant defense enzyme in cellular defense against heat shock through the removal of ROS.

Authors+Show Affiliations

Department of Biochemistry, College of Natural Sciences, Kyungpook National University, Taegu, South Korea.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14703799

Citation

Choi, In Youl, and Jeen-Woo Park. "Sensitization of U937 Cells to Heat Shock By Oxalomalate, a Competitive Inhibitor of NADP+-dependent Isocitrate Dehydrogenase." Free Radical Research, vol. 37, no. 10, 2003, pp. 1099-105.
Choi IY, Park JW. Sensitization of U937 cells to heat shock by oxalomalate, a competitive inhibitor of NADP+-dependent isocitrate dehydrogenase. Free Radic Res. 2003;37(10):1099-105.
Choi, I. Y., & Park, J. W. (2003). Sensitization of U937 cells to heat shock by oxalomalate, a competitive inhibitor of NADP+-dependent isocitrate dehydrogenase. Free Radical Research, 37(10), 1099-105.
Choi IY, Park JW. Sensitization of U937 Cells to Heat Shock By Oxalomalate, a Competitive Inhibitor of NADP+-dependent Isocitrate Dehydrogenase. Free Radic Res. 2003;37(10):1099-105. PubMed PMID: 14703799.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sensitization of U937 cells to heat shock by oxalomalate, a competitive inhibitor of NADP+-dependent isocitrate dehydrogenase. AU - Choi,In Youl, AU - Park,Jeen-Woo, PY - 2004/1/6/pubmed PY - 2004/6/3/medline PY - 2004/1/6/entrez SP - 1099 EP - 105 JF - Free radical research JO - Free Radic Res VL - 37 IS - 10 N2 - Heat shock may increase oxidative stress due to increased production of reactive oxygen species (ROS) and/or the promotion of cellular oxidation events. Cytosolic NADP+-dependent isocitrate dehydrogenase (ICDH) in U937 cells produces NADPH, an essential reducing equivalent for the antioxidant system. The protective role of ICDH against heat shock in U937 cells was investigated in control and cells treated with oxlalomalate, a competitive inhibitor of ICDH. Upon exposure to heat shock, the viability was lower and the protein oxidation, lipid peroxidation and oxidative DNA damage were higher in oxalomalate-treated cells as compared to control cells. We also observed the significant increase in the endogenous production of ROS, as measured by the oxidation of 2'7'-dichlorodihydrofluorescin in U937 cells treated with oxalomalate. These results suggest that ICDH plays an important role as an antioxidant defense enzyme in cellular defense against heat shock through the removal of ROS. SN - 1071-5762 UR - https://www.unboundmedicine.com/medline/citation/14703799/Sensitization_of_U937_cells_to_heat_shock_by_oxalomalate_a_competitive_inhibitor_of_NADP+_dependent_isocitrate_dehydrogenase_ L2 - https://www.tandfonline.com/doi/full/10.1080/10715760310001601047 DB - PRIME DP - Unbound Medicine ER -