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Improved HPLC determination of phenolic compounds in cv. golden delicious apples using a monolithic column.
J Agric Food Chem. 2004 Jan 14; 52(1):3-7.JA

Abstract

A rapid HPLC-DAD determination of phenols in apple using an RP monolithic column is reported. Because of the hydrodynamic advantages offered by this kind of column and the use of acidified acetonitrile as eluent, assays of apple extracts can be performed in <21 min. Assays of pulp and peel extracts were carried out without the need for time-consuming sample pretreatment except filtration. Several flavanols, hydroxycinnamic acids, dihydrochalcones, and six quercetin glycosides were identified and quantified. A seventh quercetin derivative, two chalcone-related compounds, and three hydroxycinnamic derivatives were also found. Peels proved to be richer in phenols than pulps, the former being composed mainly of (-)-epicatechin, procyanidin B2, chlorogenic acid, phloridzin, hyperin, and avicularin. In pulps, where the chlorogenic acid was the principal phenolic compound, quercetin glycosides were found in very low amounts.

Authors+Show Affiliations

Chinnici F
Dipartimento di Scienze degli Alimenti, Università di Bologna, 40127 Bologna, Italy. chinnici@agrsci.unibo.it
Gaiani A
No affiliation info available
Natali N
No affiliation info available
Riponi C
No affiliation info available
Galassi S
No affiliation info available

MeSH

AcetonitrilesBiflavonoidsCatechinChromatography, High Pressure LiquidFlavonolsFruitMalusPhenolsProanthocyanidins

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14709005

Citation

Chinnici, Fabio, et al. "Improved HPLC Determination of Phenolic Compounds in Cv. Golden Delicious Apples Using a Monolithic Column." Journal of Agricultural and Food Chemistry, vol. 52, no. 1, 2004, pp. 3-7.
Chinnici F, Gaiani A, Natali N, et al. Improved HPLC determination of phenolic compounds in cv. golden delicious apples using a monolithic column. J Agric Food Chem. 2004;52(1):3-7.
Chinnici, F., Gaiani, A., Natali, N., Riponi, C., & Galassi, S. (2004). Improved HPLC determination of phenolic compounds in cv. golden delicious apples using a monolithic column. Journal of Agricultural and Food Chemistry, 52(1), 3-7.
Chinnici F, et al. Improved HPLC Determination of Phenolic Compounds in Cv. Golden Delicious Apples Using a Monolithic Column. J Agric Food Chem. 2004 Jan 14;52(1):3-7. PubMed PMID: 14709005.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improved HPLC determination of phenolic compounds in cv. golden delicious apples using a monolithic column. AU - Chinnici,Fabio, AU - Gaiani,Anna, AU - Natali,Nadia, AU - Riponi,Claudio, AU - Galassi,Sergio, PY - 2004/1/8/pubmed PY - 2004/2/18/medline PY - 2004/1/8/entrez SP - 3 EP - 7 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 52 IS - 1 N2 - A rapid HPLC-DAD determination of phenols in apple using an RP monolithic column is reported. Because of the hydrodynamic advantages offered by this kind of column and the use of acidified acetonitrile as eluent, assays of apple extracts can be performed in <21 min. Assays of pulp and peel extracts were carried out without the need for time-consuming sample pretreatment except filtration. Several flavanols, hydroxycinnamic acids, dihydrochalcones, and six quercetin glycosides were identified and quantified. A seventh quercetin derivative, two chalcone-related compounds, and three hydroxycinnamic derivatives were also found. Peels proved to be richer in phenols than pulps, the former being composed mainly of (-)-epicatechin, procyanidin B2, chlorogenic acid, phloridzin, hyperin, and avicularin. In pulps, where the chlorogenic acid was the principal phenolic compound, quercetin glycosides were found in very low amounts. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/14709005/Improved_HPLC_determination_of_phenolic_compounds_in_cv__golden_delicious_apples_using_a_monolithic_column_ DB - PRIME DP - Unbound Medicine ER -