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Microdomains of the C-type lectin DC-SIGN are portals for virus entry into dendritic cells.
J Cell Biol. 2004 Jan 05; 164(1):145-55.JC

Abstract

The C-type lectin dendritic cell (DC)-specific intercellular adhesion molecule grabbing non-integrin (DC-SIGN; CD209) facilitates binding and internalization of several viruses, including HIV-1, on DCs, but the underlying mechanism for being such an efficient phagocytic pathogen-recognition receptor is poorly understood. By high resolution electron microscopy, we demonstrate a direct relation between DC-SIGN function as viral receptor and its microlocalization on the plasma membrane. During development of human monocyte-derived DCs, DC-SIGN becomes organized in well-defined microdomains, with an average diameter of 200 nm. Biochemical experiments and confocal microscopy indicate that DC-SIGN microdomains reside within lipid rafts. Finally, we show that the organization of DC-SIGN in microdomains on the plasma membrane is important for binding and internalization of virus particles, suggesting that these multimolecular assemblies of DC-SIGN act as a docking site for pathogens like HIV-1 to invade the host.

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Authors+Show Affiliations

Cambi A
Dept. of Tumor Immunology, Nijmegen Center for Molecular Life Sciences, University Medical Center Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, Netherlands.
de Lange F
No affiliation info available
van Maarseveen NM
No affiliation info available
Nijhuis M
No affiliation info available
Joosten B
No affiliation info available
van Dijk EM
No affiliation info available
de Bakker BI
No affiliation info available
Fransen JA
No affiliation info available
Bovee-Geurts PH
No affiliation info available
van Leeuwen FN
No affiliation info available
Van Hulst NF
No affiliation info available
Figdor CG
No affiliation info available

MeSH

Cell Adhesion MoleculesCell MembraneCells, CulturedDendritic CellsEndocytosisHIV InfectionsHIV-1HumansImmunohistochemistryLectins, C-TypeMembrane MicrodomainsMicroscopy, ElectronMonocytesProtein Structure, TertiaryRNA Virus InfectionsReceptors, Cell SurfaceReceptors, Virus

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14709546

Citation

Cambi, Alessandra, et al. "Microdomains of the C-type Lectin DC-SIGN Are Portals for Virus Entry Into Dendritic Cells." The Journal of Cell Biology, vol. 164, no. 1, 2004, pp. 145-55.
Cambi A, de Lange F, van Maarseveen NM, et al. Microdomains of the C-type lectin DC-SIGN are portals for virus entry into dendritic cells. J Cell Biol. 2004;164(1):145-55.
Cambi, A., de Lange, F., van Maarseveen, N. M., Nijhuis, M., Joosten, B., van Dijk, E. M., de Bakker, B. I., Fransen, J. A., Bovee-Geurts, P. H., van Leeuwen, F. N., Van Hulst, N. F., & Figdor, C. G. (2004). Microdomains of the C-type lectin DC-SIGN are portals for virus entry into dendritic cells. The Journal of Cell Biology, 164(1), 145-55.
Cambi A, et al. Microdomains of the C-type Lectin DC-SIGN Are Portals for Virus Entry Into Dendritic Cells. J Cell Biol. 2004 Jan 5;164(1):145-55. PubMed PMID: 14709546.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microdomains of the C-type lectin DC-SIGN are portals for virus entry into dendritic cells. AU - Cambi,Alessandra, AU - de Lange,Frank, AU - van Maarseveen,Noortje M, AU - Nijhuis,Monique, AU - Joosten,Ben, AU - van Dijk,Erik M H P, AU - de Bakker,Bärbel I, AU - Fransen,Jack A M, AU - Bovee-Geurts,Petra H M, AU - van Leeuwen,Frank N, AU - Van Hulst,Niek F, AU - Figdor,Carl G, PY - 2004/1/8/pubmed PY - 2004/9/3/medline PY - 2004/1/8/entrez SP - 145 EP - 55 JF - The Journal of cell biology JO - J Cell Biol VL - 164 IS - 1 N2 - The C-type lectin dendritic cell (DC)-specific intercellular adhesion molecule grabbing non-integrin (DC-SIGN; CD209) facilitates binding and internalization of several viruses, including HIV-1, on DCs, but the underlying mechanism for being such an efficient phagocytic pathogen-recognition receptor is poorly understood. By high resolution electron microscopy, we demonstrate a direct relation between DC-SIGN function as viral receptor and its microlocalization on the plasma membrane. During development of human monocyte-derived DCs, DC-SIGN becomes organized in well-defined microdomains, with an average diameter of 200 nm. Biochemical experiments and confocal microscopy indicate that DC-SIGN microdomains reside within lipid rafts. Finally, we show that the organization of DC-SIGN in microdomains on the plasma membrane is important for binding and internalization of virus particles, suggesting that these multimolecular assemblies of DC-SIGN act as a docking site for pathogens like HIV-1 to invade the host. SN - 0021-9525 UR - https://www.unboundmedicine.com/medline/citation/14709546/Microdomains_of_the_C_type_lectin_DC_SIGN_are_portals_for_virus_entry_into_dendritic_cells_ DB - PRIME DP - Unbound Medicine ER -