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Laboratory diagnosis of enteroviral infections of the central nervous system by using a nested RT-polymerase chain reaction (PCR) assay.
Diagn Microbiol Infect Dis. 2003 Dec; 47(4):557-62.DM

Abstract

Enteroviruses are the most common pathogens identified in infants hospitalized for suspected aseptic meningitis. Rapid detection of enterovirus infection is essential in taking the decision for treatment with antiviral agents and applying infection control measures in hospitalized pediatric patients. The purpose of this study was to compare the results of conventional virus isolation with those of enteroviral RNA detection by reverse transcription (RT)-PCR method in identical specimens from cases of suspected aseptic meningitis. Cerebrospinal fluid (CSF) samples were collected for viral examination from 68 pediatric patients with suspected aseptic meningitis from 1999 to 2002. These samples were inoculated in HeLa, Hep-2 and RD cell culture. The viral RNA was investigated by in-house RT-PCR method. The isolated viruses were typed by neutralization test. 36 of the 68 specimens were detected to be enterovirus positive by culture method, while 43 of them yielded positive results when RT-PCR method is used. Discrepancies occurred between the two methods in 15 specimens. While 11 specimens were positive by RT-PCR, these are found to be culture-negative. The isolated viruses were typed as Echovirus 30 (n: 30), Group B coxsackievirus (n: 5) and one isolate could not be typed by neutralization. Because of higher sensitivity and rapidity of RT-PCR, it is superior (p = 0.016) to virus culture of CSF for the diagnosis of enterovirus meningitis. Although the clinical usefulness of viral culture from CSF is limited, the final laboratory identification needs cultural techniques.

Authors+Show Affiliations

Division of Virology, Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy and School of Medicine, 06018 Ankara, Turkey. cguney@gata.edu.trNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

14711475

Citation

Guney, Cakir, et al. "Laboratory Diagnosis of Enteroviral Infections of the Central Nervous System By Using a Nested RT-polymerase Chain Reaction (PCR) Assay." Diagnostic Microbiology and Infectious Disease, vol. 47, no. 4, 2003, pp. 557-62.
Guney C, Ozkaya E, Yapar M, et al. Laboratory diagnosis of enteroviral infections of the central nervous system by using a nested RT-polymerase chain reaction (PCR) assay. Diagn Microbiol Infect Dis. 2003;47(4):557-62.
Guney, C., Ozkaya, E., Yapar, M., Gumus, I., Kubar, A., & Doganci, L. (2003). Laboratory diagnosis of enteroviral infections of the central nervous system by using a nested RT-polymerase chain reaction (PCR) assay. Diagnostic Microbiology and Infectious Disease, 47(4), 557-62.
Guney C, et al. Laboratory Diagnosis of Enteroviral Infections of the Central Nervous System By Using a Nested RT-polymerase Chain Reaction (PCR) Assay. Diagn Microbiol Infect Dis. 2003;47(4):557-62. PubMed PMID: 14711475.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Laboratory diagnosis of enteroviral infections of the central nervous system by using a nested RT-polymerase chain reaction (PCR) assay. AU - Guney,Cakir, AU - Ozkaya,Etem, AU - Yapar,Mehmet, AU - Gumus,Ismail, AU - Kubar,Ayhan, AU - Doganci,Levent, PY - 2004/1/9/pubmed PY - 2004/2/20/medline PY - 2004/1/9/entrez SP - 557 EP - 62 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 47 IS - 4 N2 - Enteroviruses are the most common pathogens identified in infants hospitalized for suspected aseptic meningitis. Rapid detection of enterovirus infection is essential in taking the decision for treatment with antiviral agents and applying infection control measures in hospitalized pediatric patients. The purpose of this study was to compare the results of conventional virus isolation with those of enteroviral RNA detection by reverse transcription (RT)-PCR method in identical specimens from cases of suspected aseptic meningitis. Cerebrospinal fluid (CSF) samples were collected for viral examination from 68 pediatric patients with suspected aseptic meningitis from 1999 to 2002. These samples were inoculated in HeLa, Hep-2 and RD cell culture. The viral RNA was investigated by in-house RT-PCR method. The isolated viruses were typed by neutralization test. 36 of the 68 specimens were detected to be enterovirus positive by culture method, while 43 of them yielded positive results when RT-PCR method is used. Discrepancies occurred between the two methods in 15 specimens. While 11 specimens were positive by RT-PCR, these are found to be culture-negative. The isolated viruses were typed as Echovirus 30 (n: 30), Group B coxsackievirus (n: 5) and one isolate could not be typed by neutralization. Because of higher sensitivity and rapidity of RT-PCR, it is superior (p = 0.016) to virus culture of CSF for the diagnosis of enterovirus meningitis. Although the clinical usefulness of viral culture from CSF is limited, the final laboratory identification needs cultural techniques. SN - 0732-8893 UR - https://www.unboundmedicine.com/medline/citation/14711475/Laboratory_diagnosis_of_enteroviral_infections_of_the_central_nervous_system_by_using_a_nested_RT_polymerase_chain_reaction__PCR__assay_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732889303001482 DB - PRIME DP - Unbound Medicine ER -