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Recombinant lipid transfer protein Cor a 8 from hazelnut: a new tool for in vitro diagnosis of potentially severe hazelnut allergy.
J Allergy Clin Immunol. 2004 Jan; 113(1):141-7.JA

Abstract

BACKGROUND

Cor a 1.04 has been identified as the major hazelnut allergen in 65 European patients with positive double-blind, placebo-controlled food challenge results to hazelnut. Recently, the 11S globulin Cor a 9 was shown to be a pollen-independent hazelnut allergen in the United States, whereas preliminary data suggest the lipid transfer protein (LTP) as an important birch pollen-unrelated hazelnut allergen in Europe.

OBJECTIVE

We sought to recruit a group of European patients allergic to hazelnut without birch pollen allergy and to identify and clone the major food allergen(s) in this study population.

METHODS

We recruited 26 such Spanish patients, including 10 patients with anaphylaxis. IgE immunoblotting was performed with hazelnut extract. Hazelnut LTP Cor a 8 was cloned by using a PCR strategy, purified, and subjected to IgE immunoblotting. Recombinant Cor a 8, rCor a 1.0401, and rCor a 2 (profilin) were further investigated by means of enzyme allergosorbent test. Immunoblot inhibition experiments were used to compare the immunologic properties of natural and recombinant LTP.

RESULTS

A 9-kd major allergen was identified in hazelnut extract. Cloning, sequencing, heterologous expression, and inhibition experiments identified it as an LTP. The prevalence of specific IgE antibody reactivity to LTP was 62% in hazelnut extract and 77% when recombinant LTP was tested by means of immunoblotting. IgE immunoblot inhibition with hazelnut extract showed that natural Cor a 8 and rCor a 8 shared identical epitopes. Only one patient had positive reactivity to Cor a 1.04, and no patients had positive reactivity to Cor a 2. Two sera bound to high-molecular-weight allergens. The LTP was denominated as Cor a 8 and submitted to the allergen database of the World Health Organization/International Union of Immunological Societies Allergen Nomenclature Subcommittee.

CONCLUSIONS

Cor a 8 is a relevant allergen for a majority of Spanish patients with hazelnut allergy that can cause severe allergic reactions.

Authors+Show Affiliations

Forschungszentrum Borstel, Division of Biochemical and Molecular Allergology, Borstel, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

14713920

Citation

Schocker, Frauke, et al. "Recombinant Lipid Transfer Protein Cor a 8 From Hazelnut: a New Tool for in Vitro Diagnosis of Potentially Severe Hazelnut Allergy." The Journal of Allergy and Clinical Immunology, vol. 113, no. 1, 2004, pp. 141-7.
Schocker F, Lüttkopf D, Scheurer S, et al. Recombinant lipid transfer protein Cor a 8 from hazelnut: a new tool for in vitro diagnosis of potentially severe hazelnut allergy. J Allergy Clin Immunol. 2004;113(1):141-7.
Schocker, F., Lüttkopf, D., Scheurer, S., Petersen, A., Cisteró-Bahima, A., Enrique, E., San Miguel-Moncín, M., Akkerdaas, J., van Ree, R., Vieths, S., & Becker, W. M. (2004). Recombinant lipid transfer protein Cor a 8 from hazelnut: a new tool for in vitro diagnosis of potentially severe hazelnut allergy. The Journal of Allergy and Clinical Immunology, 113(1), 141-7.
Schocker F, et al. Recombinant Lipid Transfer Protein Cor a 8 From Hazelnut: a New Tool for in Vitro Diagnosis of Potentially Severe Hazelnut Allergy. J Allergy Clin Immunol. 2004;113(1):141-7. PubMed PMID: 14713920.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Recombinant lipid transfer protein Cor a 8 from hazelnut: a new tool for in vitro diagnosis of potentially severe hazelnut allergy. AU - Schocker,Frauke, AU - Lüttkopf,Dirk, AU - Scheurer,Stephan, AU - Petersen,Arnd, AU - Cisteró-Bahima,Anna, AU - Enrique,Ernesto, AU - San Miguel-Moncín,Mar, AU - Akkerdaas,Jaap, AU - van Ree,Ronald, AU - Vieths,Stefan, AU - Becker,Wolf-Meinhard, PY - 2004/1/10/pubmed PY - 2004/2/18/medline PY - 2004/1/10/entrez SP - 141 EP - 7 JF - The Journal of allergy and clinical immunology JO - J Allergy Clin Immunol VL - 113 IS - 1 N2 - BACKGROUND: Cor a 1.04 has been identified as the major hazelnut allergen in 65 European patients with positive double-blind, placebo-controlled food challenge results to hazelnut. Recently, the 11S globulin Cor a 9 was shown to be a pollen-independent hazelnut allergen in the United States, whereas preliminary data suggest the lipid transfer protein (LTP) as an important birch pollen-unrelated hazelnut allergen in Europe. OBJECTIVE: We sought to recruit a group of European patients allergic to hazelnut without birch pollen allergy and to identify and clone the major food allergen(s) in this study population. METHODS: We recruited 26 such Spanish patients, including 10 patients with anaphylaxis. IgE immunoblotting was performed with hazelnut extract. Hazelnut LTP Cor a 8 was cloned by using a PCR strategy, purified, and subjected to IgE immunoblotting. Recombinant Cor a 8, rCor a 1.0401, and rCor a 2 (profilin) were further investigated by means of enzyme allergosorbent test. Immunoblot inhibition experiments were used to compare the immunologic properties of natural and recombinant LTP. RESULTS: A 9-kd major allergen was identified in hazelnut extract. Cloning, sequencing, heterologous expression, and inhibition experiments identified it as an LTP. The prevalence of specific IgE antibody reactivity to LTP was 62% in hazelnut extract and 77% when recombinant LTP was tested by means of immunoblotting. IgE immunoblot inhibition with hazelnut extract showed that natural Cor a 8 and rCor a 8 shared identical epitopes. Only one patient had positive reactivity to Cor a 1.04, and no patients had positive reactivity to Cor a 2. Two sera bound to high-molecular-weight allergens. The LTP was denominated as Cor a 8 and submitted to the allergen database of the World Health Organization/International Union of Immunological Societies Allergen Nomenclature Subcommittee. CONCLUSIONS: Cor a 8 is a relevant allergen for a majority of Spanish patients with hazelnut allergy that can cause severe allergic reactions. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/14713920/Recombinant_lipid_transfer_protein_Cor_a_8_from_hazelnut:_a_new_tool_for_in_vitro_diagnosis_of_potentially_severe_hazelnut_allergy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091674903022759 DB - PRIME DP - Unbound Medicine ER -