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Soybean allergy in patients allergic to birch pollen: clinical investigation and molecular characterization of allergens.
J Allergy Clin Immunol 2004; 113(1):148-54JA

Abstract

BACKGROUND

Allergic reactions to legumes are generally thought to be acquired by means of primary sensitization through the gastrointestinal tract. Recently, Gly m 4 (starvation-associated message 22), a Bet v 1-related pathogenesis-related protein 10 from soy, was suggested to be an allergen in patients with allergic reactions to a dietary product containing a soy protein isolate.

OBJECTIVE

We sought to evaluate the clinical relevance of Gly m 4 in subjects allergic to birch pollen with soy allergy and to assess the risk for subjects allergic to birch pollen to acquire soy allergy.

METHODS

Twenty-two patients allergic to birch pollen with soy allergy confirmed by means of positive double-blind, placebo-controlled food challenge results (n = 16) or a convincing history (n = 6) were investigated for IgE reactivity to birch pollen and soy allergens by using the Pharmacia CAP system and immunoblot analysis. Cross-reactivity was assessed by means of enzyme allergosorbent test inhibition. Ninety-four patients with birch pollen allergy were interviewed to assess soy tolerance and screened for IgE reactivity to Gly m 4 by means of immunoblotting. The Gly m 4 content in soy foods and soybean varieties was investigated by means of quantitative evaluation of immunoblots.

RESULTS

During double-blind, placebo-controlled food challenge, 10 patients experienced symptoms localized to the oral cavity, and 6 patients had a more severe reaction. CAP analysis revealed Gly m 4-specific IgE in 96% (21/22) of the patients. All patients had Bet v 1-specific IgE antibodies, and 23% (5/22) had positive Bet v 2 results. In IgE immunoblotting 25% (6/22) of the patients recognized soy profilin (Gly m 3), and 64% (14/22) recognized other soy proteins. IgE binding to soy was at least 80% inhibited by birch pollen and 60% inhibited by rGly m 4 in 9 of 11 sera tested. Seventy-one percent (67/94) of highly Bet v 1-sensitized patients with birch pollen allergy were sensitized to Gly m 4, and 9 (9.6%) of those patients reported soy allergy. The Gly m 4 content in soy products ranged between 0 and 70 ppm (milligrams per kilogram).

CONCLUSIONS

Our results confirm that soybean is another birch pollen-related allergenic food. Gly m 4 is the major soy allergen for patients allergic to birch pollen with soy allergy. The content of Gly m 4 in soy food products strongly depends on the degree of food processing.

Authors+Show Affiliations

Department of Dermatology, University Hospital, Zurich, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14713921

Citation

Mittag, Diana, et al. "Soybean Allergy in Patients Allergic to Birch Pollen: Clinical Investigation and Molecular Characterization of Allergens." The Journal of Allergy and Clinical Immunology, vol. 113, no. 1, 2004, pp. 148-54.
Mittag D, Vieths S, Vogel L, et al. Soybean allergy in patients allergic to birch pollen: clinical investigation and molecular characterization of allergens. J Allergy Clin Immunol. 2004;113(1):148-54.
Mittag, D., Vieths, S., Vogel, L., Becker, W. M., Rihs, H. P., Helbling, A., ... Ballmer-Weber, B. K. (2004). Soybean allergy in patients allergic to birch pollen: clinical investigation and molecular characterization of allergens. The Journal of Allergy and Clinical Immunology, 113(1), pp. 148-54.
Mittag D, et al. Soybean Allergy in Patients Allergic to Birch Pollen: Clinical Investigation and Molecular Characterization of Allergens. J Allergy Clin Immunol. 2004;113(1):148-54. PubMed PMID: 14713921.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Soybean allergy in patients allergic to birch pollen: clinical investigation and molecular characterization of allergens. AU - Mittag,Diana, AU - Vieths,Stefan, AU - Vogel,Lothar, AU - Becker,Wolf-Meinhard, AU - Rihs,Hans-Peter, AU - Helbling,Arthur, AU - Wüthrich,Brunello, AU - Ballmer-Weber,Barbara K, PY - 2004/1/10/pubmed PY - 2004/2/18/medline PY - 2004/1/10/entrez SP - 148 EP - 54 JF - The Journal of allergy and clinical immunology JO - J. Allergy Clin. Immunol. VL - 113 IS - 1 N2 - BACKGROUND: Allergic reactions to legumes are generally thought to be acquired by means of primary sensitization through the gastrointestinal tract. Recently, Gly m 4 (starvation-associated message 22), a Bet v 1-related pathogenesis-related protein 10 from soy, was suggested to be an allergen in patients with allergic reactions to a dietary product containing a soy protein isolate. OBJECTIVE: We sought to evaluate the clinical relevance of Gly m 4 in subjects allergic to birch pollen with soy allergy and to assess the risk for subjects allergic to birch pollen to acquire soy allergy. METHODS: Twenty-two patients allergic to birch pollen with soy allergy confirmed by means of positive double-blind, placebo-controlled food challenge results (n = 16) or a convincing history (n = 6) were investigated for IgE reactivity to birch pollen and soy allergens by using the Pharmacia CAP system and immunoblot analysis. Cross-reactivity was assessed by means of enzyme allergosorbent test inhibition. Ninety-four patients with birch pollen allergy were interviewed to assess soy tolerance and screened for IgE reactivity to Gly m 4 by means of immunoblotting. The Gly m 4 content in soy foods and soybean varieties was investigated by means of quantitative evaluation of immunoblots. RESULTS: During double-blind, placebo-controlled food challenge, 10 patients experienced symptoms localized to the oral cavity, and 6 patients had a more severe reaction. CAP analysis revealed Gly m 4-specific IgE in 96% (21/22) of the patients. All patients had Bet v 1-specific IgE antibodies, and 23% (5/22) had positive Bet v 2 results. In IgE immunoblotting 25% (6/22) of the patients recognized soy profilin (Gly m 3), and 64% (14/22) recognized other soy proteins. IgE binding to soy was at least 80% inhibited by birch pollen and 60% inhibited by rGly m 4 in 9 of 11 sera tested. Seventy-one percent (67/94) of highly Bet v 1-sensitized patients with birch pollen allergy were sensitized to Gly m 4, and 9 (9.6%) of those patients reported soy allergy. The Gly m 4 content in soy products ranged between 0 and 70 ppm (milligrams per kilogram). CONCLUSIONS: Our results confirm that soybean is another birch pollen-related allergenic food. Gly m 4 is the major soy allergen for patients allergic to birch pollen with soy allergy. The content of Gly m 4 in soy food products strongly depends on the degree of food processing. SN - 0091-6749 UR - https://www.unboundmedicine.com/medline/citation/14713921/Soybean_allergy_in_patients_allergic_to_birch_pollen:_clinical_investigation_and_molecular_characterization_of_allergens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091674903023674 DB - PRIME DP - Unbound Medicine ER -