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Malolactic bioconversion using a Oenococcus oeni strain for cider production: effect of yeast extract supplementation.
J Ind Microbiol Biotechnol. 2003 Dec; 30(12):699-704.JI

Abstract

Yeast extract addition to reconstituted apple juice had a positive impact on the development of the malolactic starter culture used to ensure malolactic fermentation in cider, using active but non-proliferating cells. In this work, the reuse of fermentation lees from cider is proposed as an alternative to the use of commercial yeast extract products. Malolactic enzymatic assays, both in whole cells and cell-free extracts, were carried out to determine the best time to harvest cells for use as an inoculum in cider. Cells harvested at the late exponential phase, the physiological stage of growth corresponding to the maximum values of specific malolactic activity, achieved a good rate of malic acid degradation in controlled cider fermentation. Under the laboratory conditions used, malic acid degradation rates in the fermentation media turned out to be near 2.0 and 2.5 times lower, compared with the rates obtained in whole-cell enzymatic assays, as useful data applicable to industrial cider production.

Authors+Show Affiliations

Department of Chemical Engineering and Environmental Technology, Faculty of Chemistry, University of Oviedo, C/Julián Clavería s/n, 33071 Oviedo, Spain.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14714193

Citation

Herrero, Mónica, et al. "Malolactic Bioconversion Using a Oenococcus Oeni Strain for Cider Production: Effect of Yeast Extract Supplementation." Journal of Industrial Microbiology & Biotechnology, vol. 30, no. 12, 2003, pp. 699-704.
Herrero M, García LA, Díaz M. Malolactic bioconversion using a Oenococcus oeni strain for cider production: effect of yeast extract supplementation. J Ind Microbiol Biotechnol. 2003;30(12):699-704.
Herrero, M., García, L. A., & Díaz, M. (2003). Malolactic bioconversion using a Oenococcus oeni strain for cider production: effect of yeast extract supplementation. Journal of Industrial Microbiology & Biotechnology, 30(12), 699-704.
Herrero M, García LA, Díaz M. Malolactic Bioconversion Using a Oenococcus Oeni Strain for Cider Production: Effect of Yeast Extract Supplementation. J Ind Microbiol Biotechnol. 2003;30(12):699-704. PubMed PMID: 14714193.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Malolactic bioconversion using a Oenococcus oeni strain for cider production: effect of yeast extract supplementation. AU - Herrero,Mónica, AU - García,Luis A, AU - Díaz,Mario, Y1 - 2004/01/09/ PY - 2003/05/05/received PY - 2003/10/31/accepted PY - 2004/1/10/pubmed PY - 2004/4/7/medline PY - 2004/1/10/entrez SP - 699 EP - 704 JF - Journal of industrial microbiology & biotechnology JO - J. Ind. Microbiol. Biotechnol. VL - 30 IS - 12 N2 - Yeast extract addition to reconstituted apple juice had a positive impact on the development of the malolactic starter culture used to ensure malolactic fermentation in cider, using active but non-proliferating cells. In this work, the reuse of fermentation lees from cider is proposed as an alternative to the use of commercial yeast extract products. Malolactic enzymatic assays, both in whole cells and cell-free extracts, were carried out to determine the best time to harvest cells for use as an inoculum in cider. Cells harvested at the late exponential phase, the physiological stage of growth corresponding to the maximum values of specific malolactic activity, achieved a good rate of malic acid degradation in controlled cider fermentation. Under the laboratory conditions used, malic acid degradation rates in the fermentation media turned out to be near 2.0 and 2.5 times lower, compared with the rates obtained in whole-cell enzymatic assays, as useful data applicable to industrial cider production. SN - 1367-5435 UR - https://www.unboundmedicine.com/medline/citation/14714193/Malolactic_bioconversion_using_a_Oenococcus_oeni_strain_for_cider_production:_effect_of_yeast_extract_supplementation_ L2 - http://dx.doi.org/10.1007/s10295-003-0102-9 DB - PRIME DP - Unbound Medicine ER -