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Quantitative photoelectrochemical detection of biological affinity reaction: biotin-avidin interaction.
Anal Chem. 2004 Jan 15; 76(2):499-501.AC

Abstract

Quantitative detection of a biological affinity reaction, the biotin/avidin recognition, was achieved using our newly developed photoelectrochemical analytical system. The system is based on the operation mechanism of the well-developed dye-sensitized photoelectrochemical solar cells and comprises a ruthenium tris(2,2'-bipyridine) (Ru-bipy) derivative as the photoelectrochemical signal-generating molecule, oxalate as the sacrificial electron donor, and tin oxide nanoparticle as the semiconductor electrode material. To perform the affinity reaction, avidin was immobilized on SnO(2) electrode by passive adsorption. Biotin-linked bovine serum albumin (BSA) was labeled with an NHS-ester derivative of Ru-bipy. After binding of BSA to the surface-immobilized avidin through biotin, photoelectrochemical measurement was carried out in the presence of oxalate. Anodic photocurrent was turned on and off repeatedly by control of incidental light. The action spectrum of the photocurrent resembled the absorption spectrum of Ru-bipy, proving the photocurrent was generated from the metal complex. A linear relationship between photocurrent and BSA concentration was obtained in the range of 1-100 microg/mL. This is the first case of quantitative photoelectrochemical detection of a biological affinity interaction.

Authors

Dong D
No affiliation info available
Zheng D
No affiliation info available
Wang FQ
No affiliation info available
Yang XQ
No affiliation info available
Wang N
No affiliation info available
Li YG
No affiliation info available
Guo LH
No affiliation info available
Cheng J
No affiliation info available

MeSH

2,2'-DipyridylAvidinBinding, CompetitiveBiosensing TechniquesBiotinColloidsElectrochemistryElectrodesLightOxalatesPhotochemistryProtein BindingRutheniumSerum Albumin, BovineTin Compounds

Pub Type(s)

Letter
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14719905

Citation

Dong, Dong, et al. "Quantitative Photoelectrochemical Detection of Biological Affinity Reaction: Biotin-avidin Interaction." Analytical Chemistry, vol. 76, no. 2, 2004, pp. 499-501.
Dong D, Zheng D, Wang FQ, et al. Quantitative photoelectrochemical detection of biological affinity reaction: biotin-avidin interaction. Anal Chem. 2004;76(2):499-501.
Dong, D., Zheng, D., Wang, F. Q., Yang, X. Q., Wang, N., Li, Y. G., Guo, L. H., & Cheng, J. (2004). Quantitative photoelectrochemical detection of biological affinity reaction: biotin-avidin interaction. Analytical Chemistry, 76(2), 499-501.
Dong D, et al. Quantitative Photoelectrochemical Detection of Biological Affinity Reaction: Biotin-avidin Interaction. Anal Chem. 2004 Jan 15;76(2):499-501. PubMed PMID: 14719905.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative photoelectrochemical detection of biological affinity reaction: biotin-avidin interaction. AU - Dong,Dong, AU - Zheng,Dong, AU - Wang,Fu-Quan, AU - Yang,Xi-Qiang, AU - Wang,Na, AU - Li,Yuan-Guang, AU - Guo,Liang-Hong, AU - Cheng,Jing, PY - 2004/1/15/pubmed PY - 2004/10/20/medline PY - 2004/1/15/entrez SP - 499 EP - 501 JF - Analytical chemistry JO - Anal Chem VL - 76 IS - 2 N2 - Quantitative detection of a biological affinity reaction, the biotin/avidin recognition, was achieved using our newly developed photoelectrochemical analytical system. The system is based on the operation mechanism of the well-developed dye-sensitized photoelectrochemical solar cells and comprises a ruthenium tris(2,2'-bipyridine) (Ru-bipy) derivative as the photoelectrochemical signal-generating molecule, oxalate as the sacrificial electron donor, and tin oxide nanoparticle as the semiconductor electrode material. To perform the affinity reaction, avidin was immobilized on SnO(2) electrode by passive adsorption. Biotin-linked bovine serum albumin (BSA) was labeled with an NHS-ester derivative of Ru-bipy. After binding of BSA to the surface-immobilized avidin through biotin, photoelectrochemical measurement was carried out in the presence of oxalate. Anodic photocurrent was turned on and off repeatedly by control of incidental light. The action spectrum of the photocurrent resembled the absorption spectrum of Ru-bipy, proving the photocurrent was generated from the metal complex. A linear relationship between photocurrent and BSA concentration was obtained in the range of 1-100 microg/mL. This is the first case of quantitative photoelectrochemical detection of a biological affinity interaction. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/14719905/Quantitative_photoelectrochemical_detection_of_biological_affinity_reaction:_biotin_avidin_interaction_ DB - PRIME DP - Unbound Medicine ER -