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Incidence of microflora and of ochratoxin A in green coffee beans (Coffea arabica).
Food Addit Contam. 2003 Dec; 20(12):1127-31.FA

Abstract

Coffee is produced in tropical countries around the Equator where climatic conditions are favourable for fungal development and mycotoxin production; however, mycotoxins do not only occur in the tropical countries. The aim was to evaluate the mycoflora and possible incidence of ochratoxin A (OTA) in 60 samples of green coffee beans from Brazil. The mycological evaluation was carried out using a conventional method and the OTA was determined using sequential phenyl silane and immunoaffinity column cleanup followed by HPLC. The detection limit was 0.2 microg kg(-1). Practically all samples (91.7%) were contaminated with moulds. The dominant fungal genus was Aspergillus, including A. niger (83.3%), A. ochraceus (53.3%) and A. flavus (25.0%). The occurrence and the levels of the genus Cladosporium (16.6%) and Penicillium (10.0%) were substantially lower than Aspergilli. Twenty samples (33.3%) of 60 were contaminated with the toxin at levels ranging from 0.2 to 7.3microg kg(-1). The average concentration was 2.38 microg kg(-1). All positive samples showed OTA levels below the limit suggested by the European Union (8 microg kg(-1)).

Authors+Show Affiliations

Martins ML
Departimento de Higiene Pública, Serviço de Micologia, Lisbon, Portugal. marina.martins@lniv.min-agricultura.pt
Martins HM
No affiliation info available
Gimeno A
No affiliation info available

MeSH

BrazilCarcinogensChromatography, High Pressure LiquidCoffeeFood ContaminationFood MicrobiologyFungiHumansOchratoxins

Pub Type(s)

Journal Article

Language

eng

PubMed ID

14726276

Citation

Martins, M L., et al. "Incidence of Microflora and of Ochratoxin a in Green Coffee Beans (Coffea Arabica)." Food Additives and Contaminants, vol. 20, no. 12, 2003, pp. 1127-31.
Martins ML, Martins HM, Gimeno A. Incidence of microflora and of ochratoxin A in green coffee beans (Coffea arabica). Food Addit Contam. 2003;20(12):1127-31.
Martins, M. L., Martins, H. M., & Gimeno, A. (2003). Incidence of microflora and of ochratoxin A in green coffee beans (Coffea arabica). Food Additives and Contaminants, 20(12), 1127-31.
Martins ML, Martins HM, Gimeno A. Incidence of Microflora and of Ochratoxin a in Green Coffee Beans (Coffea Arabica). Food Addit Contam. 2003;20(12):1127-31. PubMed PMID: 14726276.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Incidence of microflora and of ochratoxin A in green coffee beans (Coffea arabica). AU - Martins,M L, AU - Martins,H M, AU - Gimeno,A, PY - 2004/1/17/pubmed PY - 2004/3/6/medline PY - 2004/1/17/entrez SP - 1127 EP - 31 JF - Food additives and contaminants JO - Food Addit Contam VL - 20 IS - 12 N2 - Coffee is produced in tropical countries around the Equator where climatic conditions are favourable for fungal development and mycotoxin production; however, mycotoxins do not only occur in the tropical countries. The aim was to evaluate the mycoflora and possible incidence of ochratoxin A (OTA) in 60 samples of green coffee beans from Brazil. The mycological evaluation was carried out using a conventional method and the OTA was determined using sequential phenyl silane and immunoaffinity column cleanup followed by HPLC. The detection limit was 0.2 microg kg(-1). Practically all samples (91.7%) were contaminated with moulds. The dominant fungal genus was Aspergillus, including A. niger (83.3%), A. ochraceus (53.3%) and A. flavus (25.0%). The occurrence and the levels of the genus Cladosporium (16.6%) and Penicillium (10.0%) were substantially lower than Aspergilli. Twenty samples (33.3%) of 60 were contaminated with the toxin at levels ranging from 0.2 to 7.3microg kg(-1). The average concentration was 2.38 microg kg(-1). All positive samples showed OTA levels below the limit suggested by the European Union (8 microg kg(-1)). SN - 0265-203X UR - https://www.unboundmedicine.com/medline/citation/14726276/Incidence_of_microflora_and_of_ochratoxin_A_in_green_coffee_beans__Coffea_arabica__ DB - PRIME DP - Unbound Medicine ER -