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Characterisation and marker development for low molecular weight glutenin genes from Glu-A3 alleles of bread wheat (Triticum aestivum. L).
Theor Appl Genet. 2004 May; 108(7):1409-19.TA

Abstract

PCR was used to amplify low-molecular-weight (LMW) glutenin genes from the Glu-A3 loci of hexaploid wheat cultivars containing different Glu-A3 alleles. The complete coding sequence of one LMW glutenin gene was obtained for each of the seven alleles Glu-A3a to Glu-A3g. Chromosome assignment of PCR products using Chinese Spring nulli-tetrasomic lines confirmed the amplified products were from chromosome 1A. All sequences were classified as LMW-i-type genes based on the presence of an N-terminal isoleucine residue and eight cysteine residues located within the C-terminal domain of the predicted, mature amino acid sequence. All genes contained a single uninterrupted open reading frame, including the sequence from the Glu-A3e allele, for which no protein product has been identified. Comparison of LMW glutenin gene sequences obtained from different alleles showed a wide range of sequence identity between the genes, with between 1 and 37 single nucleotide polymorphisms and between one and five insertion/deletion events between genes from different alleles. Allele-specific PCR markers were designed based on the DNA polymorphisms identified between the LMW glutenin genes, and these markers were validated against a panel of cultivars containing different Glu-A3 alleles. This collection of markers represents a valuable resource for use in marker-assisted breeding to select for specific alleles of this important quality-determining locus in bread wheat.

Authors+Show Affiliations

Commonwealth Scientific and Industrial Research Organisation, Plant Industry, GPO Box 1600, ACT 2601 Canberra, Australia.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14727031

Citation

Zhang, W, et al. "Characterisation and Marker Development for Low Molecular Weight Glutenin Genes From Glu-A3 Alleles of Bread Wheat (Triticum Aestivum. L)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 108, no. 7, 2004, pp. 1409-19.
Zhang W, Gianibelli MC, Rampling LR, et al. Characterisation and marker development for low molecular weight glutenin genes from Glu-A3 alleles of bread wheat (Triticum aestivum. L). Theor Appl Genet. 2004;108(7):1409-19.
Zhang, W., Gianibelli, M. C., Rampling, L. R., & Gale, K. R. (2004). Characterisation and marker development for low molecular weight glutenin genes from Glu-A3 alleles of bread wheat (Triticum aestivum. L). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 108(7), 1409-19.
Zhang W, et al. Characterisation and Marker Development for Low Molecular Weight Glutenin Genes From Glu-A3 Alleles of Bread Wheat (Triticum Aestivum. L). Theor Appl Genet. 2004;108(7):1409-19. PubMed PMID: 14727031.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterisation and marker development for low molecular weight glutenin genes from Glu-A3 alleles of bread wheat (Triticum aestivum. L). AU - Zhang,W, AU - Gianibelli,M C, AU - Rampling,L R, AU - Gale,K R, Y1 - 2004/01/16/ PY - 2003/07/11/received PY - 2003/11/24/accepted PY - 2004/1/17/pubmed PY - 2004/7/14/medline PY - 2004/1/17/entrez SP - 1409 EP - 19 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 108 IS - 7 N2 - PCR was used to amplify low-molecular-weight (LMW) glutenin genes from the Glu-A3 loci of hexaploid wheat cultivars containing different Glu-A3 alleles. The complete coding sequence of one LMW glutenin gene was obtained for each of the seven alleles Glu-A3a to Glu-A3g. Chromosome assignment of PCR products using Chinese Spring nulli-tetrasomic lines confirmed the amplified products were from chromosome 1A. All sequences were classified as LMW-i-type genes based on the presence of an N-terminal isoleucine residue and eight cysteine residues located within the C-terminal domain of the predicted, mature amino acid sequence. All genes contained a single uninterrupted open reading frame, including the sequence from the Glu-A3e allele, for which no protein product has been identified. Comparison of LMW glutenin gene sequences obtained from different alleles showed a wide range of sequence identity between the genes, with between 1 and 37 single nucleotide polymorphisms and between one and five insertion/deletion events between genes from different alleles. Allele-specific PCR markers were designed based on the DNA polymorphisms identified between the LMW glutenin genes, and these markers were validated against a panel of cultivars containing different Glu-A3 alleles. This collection of markers represents a valuable resource for use in marker-assisted breeding to select for specific alleles of this important quality-determining locus in bread wheat. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/14727031/Characterisation_and_marker_development_for_low_molecular_weight_glutenin_genes_from_Glu_A3_alleles_of_bread_wheat__Triticum_aestivum__L__ L2 - https://dx.doi.org/10.1007/s00122-003-1558-8 DB - PRIME DP - Unbound Medicine ER -