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Microemulsion electrokinetic chromatography with laser-induced fluorescence detection for sensitive determination of ephedrine and pseudoephedrine.
Electrophoresis. 2004 Jan; 25(1):74-9.E

Abstract

A selective and sensitive microemulsion electrokinetic chromatography with laser-induced fluorescence detection method was developed for the quantification of ephedrine (E) and pseudoephedrine (PE) derivatized with 4-chloro-7-nitrobenzo-2-oxa-1, 3-diazol. By a series of optimization, a running buffer composed of 20 mM borate + microemulsion (23.3 mM Sodium dodecyl sulfate/180.85 mM 1-butanol/16.4 mM n-heptane) +8% acetonitrile was applied for the separation of the derivatives. A linear relationship for E and PE was obtained in the range of 0.058-11.58 microg.mL(-1) (correlation coefficient: 0.9993 for E, 0.9995 for PE), and the detection limits for E and PE were 5.3 and 3.9 ng.mL(-1). The method was applied to the analysis of the two alkaloids in Chinese traditional herbal preparations with recoveries in the range of 96.9-105.4%.

Authors+Show Affiliations

Department of Chemistry, Lanzhou University, Lanzhou, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

14730571

Citation

Zhang, Jiyou, et al. "Microemulsion Electrokinetic Chromatography With Laser-induced Fluorescence Detection for Sensitive Determination of Ephedrine and Pseudoephedrine." Electrophoresis, vol. 25, no. 1, 2004, pp. 74-9.
Zhang J, Xie J, Liu J, et al. Microemulsion electrokinetic chromatography with laser-induced fluorescence detection for sensitive determination of ephedrine and pseudoephedrine. Electrophoresis. 2004;25(1):74-9.
Zhang, J., Xie, J., Liu, J., Tian, J., Chen, X., & Hu, Z. (2004). Microemulsion electrokinetic chromatography with laser-induced fluorescence detection for sensitive determination of ephedrine and pseudoephedrine. Electrophoresis, 25(1), 74-9.
Zhang J, et al. Microemulsion Electrokinetic Chromatography With Laser-induced Fluorescence Detection for Sensitive Determination of Ephedrine and Pseudoephedrine. Electrophoresis. 2004;25(1):74-9. PubMed PMID: 14730571.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microemulsion electrokinetic chromatography with laser-induced fluorescence detection for sensitive determination of ephedrine and pseudoephedrine. AU - Zhang,Jiyou, AU - Xie,Jianping, AU - Liu,Jiaqin, AU - Tian,Jianniao, AU - Chen,Xingguo, AU - Hu,Zhide, PY - 2004/1/20/pubmed PY - 2004/9/10/medline PY - 2004/1/20/entrez SP - 74 EP - 9 JF - Electrophoresis JO - Electrophoresis VL - 25 IS - 1 N2 - A selective and sensitive microemulsion electrokinetic chromatography with laser-induced fluorescence detection method was developed for the quantification of ephedrine (E) and pseudoephedrine (PE) derivatized with 4-chloro-7-nitrobenzo-2-oxa-1, 3-diazol. By a series of optimization, a running buffer composed of 20 mM borate + microemulsion (23.3 mM Sodium dodecyl sulfate/180.85 mM 1-butanol/16.4 mM n-heptane) +8% acetonitrile was applied for the separation of the derivatives. A linear relationship for E and PE was obtained in the range of 0.058-11.58 microg.mL(-1) (correlation coefficient: 0.9993 for E, 0.9995 for PE), and the detection limits for E and PE were 5.3 and 3.9 ng.mL(-1). The method was applied to the analysis of the two alkaloids in Chinese traditional herbal preparations with recoveries in the range of 96.9-105.4%. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/14730571/Microemulsion_electrokinetic_chromatography_with_laser_induced_fluorescence_detection_for_sensitive_determination_of_ephedrine_and_pseudoephedrine_ L2 - https://doi.org/10.1002/elps.200305643 DB - PRIME DP - Unbound Medicine ER -