Abstract
AIM
To evaluate the effect of interleukin-1 receptor antagonist(IL-1ra) on apoptosis and associated mechanism of eosinophil in guinea pig with asthma.
METHODS
A model of guinea pig with asthma was established. After inhalation of different concentrations of IL-1ra, asthma was induced in the guinea pig for 8 days, the concentration of eosinophil cationic protein (ECP) in serum and bronchoalveolar lavage fluid (BALF), IL-5 in serum, the eosinophil counts and apoptosis were assayed by radioimmunology, enzyme-linked immunosorbent assay(ELISA), fluoromicroscope and light microscope.
RESULTS
IL-1ra indirectly decreased the level of IL-5 in serum, improved the apoptosis of eosinophil(EOS) in lung, then decreased the level of ECP in serum and BALF.
CONCLUSION
Inhalation of nebulized IL-1ra showed protective effect against asthma through change of the activity and infiltration of EOS in lung.
TY - JOUR
T1 - [Effects of interleukin-1 receptor antagonist on the apoptosis of eosinophil in guinea pig with asthma].
AU - Xu,Feng,
AU - Zhang,Hong-quan,
PY - 2004/1/21/pubmed
PY - 2004/5/7/medline
PY - 2004/1/21/entrez
SP - 661
EP - 4
JF - Yao xue xue bao = Acta pharmaceutica Sinica
JO - Yao Xue Xue Bao
VL - 38
IS - 9
N2 - AIM: To evaluate the effect of interleukin-1 receptor antagonist(IL-1ra) on apoptosis and associated mechanism of eosinophil in guinea pig with asthma. METHODS: A model of guinea pig with asthma was established. After inhalation of different concentrations of IL-1ra, asthma was induced in the guinea pig for 8 days, the concentration of eosinophil cationic protein (ECP) in serum and bronchoalveolar lavage fluid (BALF), IL-5 in serum, the eosinophil counts and apoptosis were assayed by radioimmunology, enzyme-linked immunosorbent assay(ELISA), fluoromicroscope and light microscope. RESULTS: IL-1ra indirectly decreased the level of IL-5 in serum, improved the apoptosis of eosinophil(EOS) in lung, then decreased the level of ECP in serum and BALF. CONCLUSION: Inhalation of nebulized IL-1ra showed protective effect against asthma through change of the activity and infiltration of EOS in lung.
SN - 0513-4870
UR - https://www.unboundmedicine.com/medline/citation/14730914/[Effects_of_interleukin_1_receptor_antagonist_on_the_apoptosis_of_eosinophil_in_guinea_pig_with_asthma]_
DB - PRIME
DP - Unbound Medicine
ER -